Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index

Aziz, Nabil; Saleh, Ramadan; Sharma, Rakesh K.; Lewis-Jones, Iwan; Esfandiari, Navid; Thomas, Anthony J.; Agarwal, Ashok

doi:10.1016/j.fertnstert.2003.06.026

Cited by 253 publications

(191 citation statements)

References 37 publications

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“…The free radical scavenging activity of SOD, GPX and CAT protects the sperm membrane against the LPO, preserving its integrity and hence maintaining the normal sperm parameters of motility and morphology [6,7]. Studies by [2,8] have also implicated the role of antioxidants in protecting the sperm nuclear genome from oxidative strand breaks and base modifications. Therefore, the combination between antioxidant profile, determined by SOD, GPX and CAT activities, and LPO biomarker (MDA) was reported to be the better indicator of OS.…”

Section: Introductionmentioning

confidence: 99%

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Atig

Kerkeni

Saâd

et al. 2013

J Assist Reprod Genet

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Purpose To evaluate levels of sperm DNA fragmentation and enzymatic antioxidant status in seminal plasma of Tunisian fertile and infertile men in order to assess the effects of seminal oxidative stress on sperm DNA integrity and semen quality. Methods Semen samples from 100 infertile patients (40 oligoasthenoteratozoospermics, 31 teratozoospermics and 29 asthenozoospermics) and 50 fertile men (controls) were analyzed for DNA fragmentation by TUNEL assay and biochemical parameters. Seminal antioxidant activities (Superoxide dismutase, Glutathione peroxidase and Catalase) and malondialdehyde concentrations were measured spectrophotometrically.Results Sperm DNA fragmentation and malondialdehyde levels in infertile groups were more elevated than controls. Nevertheless, the activities of the antioxidant enzymes were significantly lower in abnormal groups compared to normozoospermics. Sperm DNA fragmentation was closely and positively correlated to malondialdehyde levels (r=0.37, P=0.008); meanwhile, reduced seminal antioxidant profile was negatively associated to sperm DNA fragmentation. Interestingly, we noted also that sperm DNA fragmentation was negatively correlated to sperm motility (r=−0.54, P<0.001) and positively associated to the abnormal sperm morphology (r=0.57, P=0.002). Conclusions This report revealed that increased sperm DNA fragmentation can be due to the impaired seminal enzymatic antioxidant profile and increased Lipid peroxidation. Our results sustain that the evaluation of sperm DNA fragmentation and seminal oxidative biomarkers in infertile men is recommended as a consistent prognostic tool for male infertility assessment.

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Section: Introductionmentioning

confidence: 99%

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Atig

Kerkeni

Saâd

et al. 2013

J Assist Reprod Genet

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“…Reactive oxygen species (ROS) produced in small amounts are necessary for sperm functions such as capacitation and acrosome reaction 8 . Excessive ROS production however is negatively linked to sperm function and morphology [9][10][11][12][13][14] and may be secondary to urogenital tract infections, neutrophils and macrophages infiltration 15 , radiation, chemotherapy and smoking.…”

Section: Introductionmentioning

confidence: 99%

Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay

Novotný¹,

Aziz²,

Rybar³

et al. 2013

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. The aim of this prospective study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage estimated by Sperm Chromatin Structure Assay. Methods. Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability. Results. DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Although there were similar trends of rising DNA fragmentation index and ROS production among the three groups of men, the relationship did not reach statistical significance. Conclusions. Higher values of DNA fragmentation index and high DNA stainability may also reflect developmental and/or environmental problems and not only oxidative stress.

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“…Se ha observado una correlación positiva entre la retención residual de citoplasma por los espermatozoides y la generación de ROS intracelular y se especula que un aumento de estos agentes está mediado por la acción de la enzima citosólica glucosa 6-fosfatodeshidrogenasa (27,28). De otro lado, el incremento de espermatozoides inmaduros con citoplasma residual observado en parejas infértiles, sugiere que la calidad de la espermatogénesis es menor a la de parejas fértiles (29)(30)(31). Además, existen otras posibilidades sobre la generación de ROS intracelular en el espermatozoide: i) la fuga de electrones de la cadena de transporte mitocondrial, ii) la NA-DPH oxidasa como una posible fuente de ROS y, iii) la generación de ROS por medio del oxido nítri-co (ON) en la regiones post-acrosomales y ecuatoriales (32).…”

Section: Materiales Y Métodosunclassified

Estrés oxidativo: ¿un estado celular defectuoso para la función espermática?

Mayorga-Torres

Camargo

Cadavid

et al. 2015

Rev. chil. obstet. ginecol.

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RESUMENEn los organismos vivos, las cantidades de radicales libres y especies reactivas del oxígeno (ROS) son controladas por un complejo sistema de homeostasis, capaz de mantener niveles fisiológicos de ROS necesarios para el funcionamiento y regulación de algunas biomoléculas. Paralelamente, los organismos poseen sistemas bioquímicos de protección contra el estrés oxidativo, que consiste en el desbalance entre la producción de especies químicas altamente reactivas y las defensas antioxidantes de la célula. Dicho estrés contribuye de manera importante a la etiología tanto de la senescencia celular como de algunas enfermedades. En el contexto reproductivo, las células espermáticas pasan por una serie de cambios fisiológicos durante los procesos de maduración, capacitación y fecundados, entre los que se incluyen las modificaciones de las proteínas existentes, reguladas por señales procedentes del entorno espermático, donde las ROS modulan importantes vías bioquímicas, involucradas en procesos fundamentales de la función del espermatozoide y que se pueden alterar en estados de estrés oxidativo. El objetivo de esta revisión de literatura es describir algunos de los procesos que contribuyen al estrés oxidativo y sus implicaciones sobre la funcionalidad espermática. PALABRAS CLAVE: Espermatozoide, fertilidad, especies reactivas del oxígeno, antioxidantes SUMMARYLiving organisms regulate the load of free radicals and reactive oxygen species (ROS) by a complex homeostatic system, capable of maintaining physiological levels of ROS, necessary for the action and regulation of some biomolecules. In parallel, organisms harbor biochemical protection systems against oxidative stress, consisting of an unbalance state between oxygen reactive chemical species and antioxidant defense production; this kind of biochemical stress has been shown to contribute to cellular senescence and the development of different diseases. In the reproductive field, the spermatic cells undergo a serial of physiological changes during the maturation, capacitation and fertilization process. Such changes include the modification of proteins regulated by signals from the sperm environment, where ROS modulate important biochemical pathways involved in fundamental processes of sperm function, and that could be altered under oxidative stress conditions. The objective of this review is to describe some of the processes that contribute to oxidative stress and its implications on sperm functionality.

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Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index

Cited by 253 publications

References 37 publications

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay

Estrés oxidativo: ¿un estado celular defectuoso para la función espermática?

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