2011
DOI: 10.1038/emboj.2011.63
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Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods

Abstract: Centrosomes in animal cells are dynamic organelles with a proteinaceous matrix of pericentriolar material assembled around a pair of centrioles. They organize the microtubule cytoskeleton and the mitotic spindle apparatus. Mature centrioles are essential for biogenesis of primary cilia that mediate key signalling events. Despite recent advances, the molecular basis for the plethora of processes coordinated by centrosomes is not fully understood. We have combined protein identification and localization, using P… Show more

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Cited by 298 publications
(319 citation statements)
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References 75 publications
(108 reference statements)
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“…Finally, the E3 ubiquitin ligase MIB1 (ref. 56) was identified in a centrosome proteome 57 and the deubiquitinase USP21 is shown to be required for primary cilia formation 50 . In addition, another deubiquitinase Usp33 has recently been shown to localize at the centrosome by its binding with CP110 and its activity antagonizes SCF cyclinF ubiquitination of CP110 (ref.…”
Section: Discussionmentioning
confidence: 99%
“…Finally, the E3 ubiquitin ligase MIB1 (ref. 56) was identified in a centrosome proteome 57 and the deubiquitinase USP21 is shown to be required for primary cilia formation 50 . In addition, another deubiquitinase Usp33 has recently been shown to localize at the centrosome by its binding with CP110 and its activity antagonizes SCF cyclinF ubiquitination of CP110 (ref.…”
Section: Discussionmentioning
confidence: 99%
“…13 PCM components include g-tubulin ring complexes (g-TuRCs) and the microtubules they nucleate, microtubule-associated proteins (MAPs), Homologous to AUgmin Subunits (HAUS) complexes, and several other integral and satellite proteins. 14 The composition and architecture of the centrioles have been characterized in great detail. [14][15][16] Centrioles are proximodistally polarized, radially symmetric, cylindrical structures typically composed of nine microtubule triplets, lumenal proteins such as centrin-2 and 3, and distal components such as centriolin.…”
Section: The Centrosome: a Miniscule Marvel And Headquarters Of Cellumentioning
confidence: 99%
“…[14][15][16] Centrioles are proximodistally polarized, radially symmetric, cylindrical structures typically composed of nine microtubule triplets, lumenal proteins such as centrin-2 and 3, and distal components such as centriolin. [14][15][16] Recent research has revealed that the mother centriole is perhaps not a bonafide template for daughter centriole formation; rather, the mother is a platform for the expeditious recruitment of duplication factors to the PCM and/or their activation within it. 17 Furthermore, the mother centriole organizes and stabilizes the PCM and regulates its size.…”
Section: The Centrosome: a Miniscule Marvel And Headquarters Of Cellumentioning
confidence: 99%
“…6 Modern imaging techniques and proteomics techniques have allowed the identification of the detailed building structure of cilia and the existence of many structural (e.g., tubulin and SAS-6) and signaling (e.g., plk4) proteins in the cilia. 10,[22][23][24] However, the regulation of ciliogenesis is still not fully understood.…”
Section: Discussionmentioning
confidence: 99%