Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction

Xu, Rui; Luo, Gaoxing; Xia, Hesheng; Wang, He; Zhao, Jian; Liu, Bo; Tan, Jianglin; Zhou, Junyi; Liu, Daisong; Wang, Yuzhen; Yao, Zhen; Zhan, Rong; Yang, Sisi; Wu, Jun

doi:10.1016/j.biomaterials.2014.10.077

Cited by 320 publications

(182 citation statements)

References 27 publications

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“…22,23 Polycaprolactone (PCL) is a biocompatible and biodegradable material approved by the US Food and Drug Administration (FDA). 24,25 The electrospun PCL nanofibrous mesh possesses high porosity and good mechanical strength, which are important factors for wound dressings.…”

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confidence: 99%

“…24,25 The electrospun PCL nanofibrous mesh possesses high porosity and good mechanical strength, which are important factors for wound dressings. 22,26,27 Moreover, the high specific surface area and interwoven nanofibers endow PCL nanofibrous mesh enough space for antibacterial agents residing. 28 Due to these attributes, PCL nanofibrous mesh is a predominant substrate to accommodate NS for an antibacterial wound dressing.…”

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confidence: 99%

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Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

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Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus , Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds.

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confidence: 99%

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confidence: 99%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

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“…Briefly, the dorsal surface of the mouse was shaved, and two sets of sterile full-thickness wounds were generated using a sterile 4-mm punch tool. The survey of wound healing was performed as previously reported with minor revision [23, 24]. Wounds were left uncovered, and mice were kept individually with sterile paper bedding.…”

Section: Methodsmentioning

confidence: 99%

“…Tissues were fixed with 4% formaldehyde, embedded in paraffin, and sectioned at a thickness of 5 µm. Hematoxylin and eosin (HE) staining and histological analyses were performed, and the length of the neo-epithelium was determined using IPP 6.0 software; the measurement procedures were performed blindly by two pathologists [23, 24]. Experiments were repeated 5 times.…”

Section: Methodsmentioning

confidence: 99%

Differential Role of Rapamycin in Epidermis-Induced IL-15-IGF-1 Secretion via Activation of Akt/mTORC2

Bai¹,

Xu²,

Zhang³

et al. 2017

Cell Physiol Biochem

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Backgroud/Aims: The effects of rapamycin (RPM) on wound healing have been previously studied. However, reciprocal contradictory data have been reported, and the underlying mechanism remains unclear. This study aims to uncover differential role of RPM in regulation of wound healing and explore the possible mechanism. Methods: C57BL/6J mice and epidermal cells were treated with different doses of RPM. The wound re-epithelialization was observed by hematoxylin and eosin (HE) staining. The expression of IL-15 and IGF-1 were detected by immunohistochemistry and quantitative real-time PCR. Epidermal cell survival was determined by CCK-8 assays. Moreover, the mTORC1 and mTORC2 pathway were examined by western blot analysis. Results: This study showed that differential doses of RPM could lead to separate consequences in epidermis. Histological analyses showed that low-dose RPM promoted wound healing, and enhanced the expression of IL-15 and IGF-1. Furthermore, western blot analysis showed that the effect of low-dose RPM in epidermis were not through mTORC1 pathway. Instead, activation of the Akt/mTORC2 pathway was involved in low-dose RPM-induced IL-15 and IGF-1 production in epidermis, while high-dose RPM inhibited the expression of IL-15 and IGF-1 and the activity of mTORC1 and mTORC2 pathway. Conclusion: This study for the first time demonstrated that RPM-mediated wound healing was dose-dependent.

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“…2 In addition, these capsular voids also encourage bacterial infection and invasion as well as inflammation during longterm use. 3 Although silicone rubber implants are bioinert and workable, they have been involved in a great number of adverse reactions, sometimes occurring decades after implantation, and to date no satisfactory solution to the problems of fibrosis and capsule formation has been found.…”

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confidence: 99%

Biofunctionalization of silicone rubber with microgroove-patterned surface and carbon-ion implantation to enhance biocompatibility and reduce capsule formation

Lei

Liu

et al. 2016

IJN

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Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction

Cited by 320 publications

References 27 publications

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Differential Role of Rapamycin in Epidermis-Induced IL-15-IGF-1 Secretion via Activation of Akt/mTORC2

Biofunctionalization of silicone rubber with microgroove-patterned surface and carbon-ion implantation to enhance biocompatibility and reduce capsule formation

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