Novel Bimolecular Fluorescence Complementation (BiFC) Assay for Visualization of the Protein–Protein Interactions and Cellular Protein Complex Localizations

Shi, Zhonggang; Gao, Xing; Zhang, Wenrui; Chen, Binghong; Wang, Mengying; Liao, Keman; Wang, Zhihan; Ren, Li; Zhai, Yujia; Qiu, Yongming; Wang, Xuhui; Lin, Yingying

doi:10.1007/s12033-023-00860-6

Mol Biotechnol

2023

DOI: 10.1007/s12033-023-00860-6

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Novel Bimolecular Fluorescence Complementation (BiFC) Assay for Visualization of the Protein–Protein Interactions and Cellular Protein Complex Localizations

Zhonggang Shi,

Xing Gao,

Wenrui Zhang

et al.

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Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein–Protein Interactions in Cell Signaling Pathways

Ren,

Ou,

et al. 2024

Biomolecules

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Signaling pathways are responsible for transmitting information between cells and regulating cell growth, differentiation, and death. Proteins in cells form complexes by interacting with each other through specific structural domains, playing a crucial role in various biological functions and cell signaling pathways. Protein–protein interactions (PPIs) within cell signaling pathways are essential for signal transmission and regulation. The spatiotemporal features of PPIs in signaling pathways are crucial for comprehending the regulatory mechanisms of signal transduction. Bimolecular fluorescence complementation (BiFC) is one kind of imaging tool for the direct visualization of PPIs in living cells and has been widely utilized to uncover novel PPIs in various organisms. BiFC demonstrates significant potential for application in various areas of biological research, drug development, disease diagnosis and treatment, and other related fields. This review systematically summarizes and analyzes the technical advancement of BiFC and its utilization in elucidating PPIs within established cell signaling pathways, including TOR, PI3K/Akt, Wnt/β-catenin, NF-κB, and MAPK. Additionally, it explores the application of this technology in revealing PPIs within the plant hormone signaling pathways of ethylene, auxin, Gibberellin, and abscisic acid. Using BiFC in conjunction with CRISPR-Cas9, live-cell imaging, and ultra-high-resolution microscopy will enhance our comprehension of PPIs in cell signaling pathways.

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Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein–Protein Interactions in Cell Signaling Pathways

Ren,

Ou,

et al. 2024

Biomolecules

View full text Add to dashboard Cite

show abstract

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Novel Bimolecular Fluorescence Complementation (BiFC) Assay for Visualization of the Protein–Protein Interactions and Cellular Protein Complex Localizations

Cited by 1 publication

References 25 publications

Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein–Protein Interactions in Cell Signaling Pathways

Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein–Protein Interactions in Cell Signaling Pathways

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