Novel bioadhesive hyaluronan–itaconic acid crosslinked films for ocular therapy

Calles, Javier Adrián; Tártara, Luis Ignacio; López-García, Antonio; Diebold, Yolanda; Palma, Santiago Daniel; Vallés, Enrique M.

doi:10.1016/j.ijpharm.2013.07.063

Cited by 43 publications

(24 citation statements)

References 39 publications

(35 reference statements)

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“…This was confirmed also by histological analysis, which showed the restoring of microscopic epithelial structure after treatment with S1 and S2. Our findings were in agreement with the results of previous in vitro and in vitro studies, which showed that corneal epithelial wound healing is promoted by native HA [ 10 , 11 , 12 , 36 , 37 , 38 ] and others type of HA-CL [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. Moreover, western blot analysis evidenced that, after the treatment with S1 and S2 eye drops, the level of the proliferative marker cyclin D1 was increased compared to the control.…”

Section: Discussionsupporting

confidence: 93%

“…Cross-linking is a chemical strategy with the aims to increase the rigidity of the polymer network (i.e., the gel viscoelasiticity), extend its permanence in the site of application and decrease its susceptibility to enzymatic degradation, thus reducing the daily number applications of a formulation [ 21 ]. To the best of our knowledge, only a few recent literature reports describe the effects of ophthalmic formulations –hydrogels, films, artificial tears-containing HA-CL for veterinary [ 22 , 23 , 24 , 25 , 26 ] and human uses [ 27 , 28 , 29 , 30 ]. These studies show promising results that open interesting perspectives to the ocular administration of cross-linked hyaluronans.…”

Section: Introductionmentioning

confidence: 99%

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Novel Artificial Tears Containing Cross-Linked Hyaluronic Acid: An In Vitro Re-Epithelialization Study

et al. 2017

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Dry eye syndrome is a common disease which can damage the corneal epithelium. It is treated with eye drops to stimulate tear production and hydrate the corneal surface. The most prescribed artificial tear remedies contain hyaluronic acid (HA), which enhances epithelial wound healing, improving tissue health. To the best of our knowledge, only a few recent studies have investigated cross-linked HA (HA-CL) in eye drops for human applications. This work consists in an in vitro evaluation of the re-epithelialization ability of two different preparations containing a recently synthetized HA cross-linked with urea: 0.02% (w/v) HA-CL (solution 1, S1), and 0.4% (w/v) HA-CL (solution 2, S2). The study was conducted on both 2D human corneal cells (HCEpiC) and 3D reconstructed tissues of human corneal epithelium (HCE). Viability by 3(4,5-dimethylthiazol-2)2,5-diphenyltetrazolium bromide (MTT) test, pro-inflammatory cytokine release (interleukin-8, IL-8) by ELISA, and morphology by hematoxylin and eosin (HE) staining were evaluated. In addition, to understand the molecular basis of the re-epithelialization properties, cyclin D1 levels were assessed by western blot. The results showed no cellular toxicity, a slight decrease in IL-8 release, and restoration of epithelium integrity when the wounded 3D model was treated with S1 and S2. In parallel, cyclin D1 levels increased in cells treated with both S1 and S2.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Novel Artificial Tears Containing Cross-Linked Hyaluronic Acid: An In Vitro Re-Epithelialization Study

et al. 2017

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“…The potential ocular irritancy and damaging effects of the thiolated conjugates were evaluated using a slightly modified version of the Draize test. 19 For ocular irritation test, 0.5% solution of cetirizine and cetirizine incorporated in a-CD-Cys2 were prepared in saline. Subsequently,100 mL of 0.1% solution prewarmed at 37 C was instilled in the conjunctival fornix of the right eye of albino rabbits using the left eye as control.…”

Section: In Vivo Evaluation Of Irritation-masking Effectmentioning

confidence: 99%

Thiolated α-Cyclodextrin: The Invisible Choice to Prolong Ocular Drug Residence Time

Ijaz

Ahmad

Akhtar

et al. 2016

Journal of Pharmaceutical Sciences

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“…Many studies have been performed to formulate HA in an injectable form [ 8 ], which can be used to treat osteoarthritis of the knee [ 9 ]. Several HA derivatives have been developed for drug delivery [ 10 ], mainly for its potential as a biodegradable carrier [ 11 ]. Some authors have reported the use of this polymer for different proteins, drugs [ 12 ], peptides [ 13 ] or for gene delivery [ 14 , 15 ] using HA as a depot system [ 16 ], as hydrogels (physically and chemically cross-linked) [ 17 – 19 ] or as nano- or micro-particulate systems [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan–Itaconic Acid–Glutaraldehyde Films for Biomedical Applications: Preliminary Studies

Calles¹,

Ressia

Llabot

et al. 2016

Sci. Pharm.

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New hyaluronic acid–itaconic acid films were synthesized as potential materials with biomedical applications. In this work, we explored the homogeneous cross-linking reactions of hyaluronic acid using glutaraldehyde in the presence of itaconic acid and triacetin as plasticizers.Biomechanical properties were assessed in terms of stability by measuring swelling in aqueous environments, investigating wettability using contact angle tests, and evaluating bioadhesive performance. The ductility of the materials was evaluated through stress-strain measurements and the morphology was explored by scanning electron microscopy.The results show that the incorporation of itaconic acid improved most of the desirable properties, increasing adhesiveness and reducing wettability and swelling. The use of triacetin enhanced the strength, bioadhesiveness, and ductility of the material.

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Novel bioadhesive hyaluronan–itaconic acid crosslinked films for ocular therapy

Cited by 43 publications

References 39 publications

Novel Artificial Tears Containing Cross-Linked Hyaluronic Acid: An In Vitro Re-Epithelialization Study

Novel Artificial Tears Containing Cross-Linked Hyaluronic Acid: An In Vitro Re-Epithelialization Study

Thiolated α-Cyclodextrin: The Invisible Choice to Prolong Ocular Drug Residence Time

Hyaluronan–Itaconic Acid–Glutaraldehyde Films for Biomedical Applications: Preliminary Studies

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