Novel concepts for improved communication between nerve cells and silicon electronic devices

Huys, Rik; Braeken, Dries; Meerbergen, Bart Van; Winters, Kurt; Eberle, Wolfgang; Loo, Josine; Tsvetanova, Diana; Chang, Cheng Jen; Severi, S.; Yitzchaik, Shlomo; Spira, Micha Ε.; Shappir, J.; Callewaert, Geert; Borghs, Gustaaf; Bartic, Carmen

doi:10.1016/j.sse.2007.10.025

Cited by 20 publications

(16 citation statements)

References 17 publications

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“…Gold spines for electrical measurements were prepared on silicon wafers as described by Huys et al (2008). Briefly, the electrodes were patterned by the Cu-damascene method.…”

mentioning

confidence: 99%

Spine-shaped gold protrusions improve the adherence and electrical coupling of neurons with the surface of micro-electronic devices

Hai

Dormann

Shappir

et al. 2009

J. R. Soc. Interface.

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137

186

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Interfacing neurons with micro-and nano-electronic devices has been a subject of intense study over the last decade. One of the major problems in assembling efficient neuroelectronic hybrid systems is the weak electrical coupling between the components. This is mainly attributed to the fundamental property of living cells to form and maintain an extracellular cleft between the plasma membrane and any substrate to which they adhere. This cleft shunts the current generated by propagating action potentials and thus reduces the signal-to-noise ratio. Reducing the cleft thickness, and thereby increasing the seal resistance formed between the neurons and the sensing surface, is thus a challenge and could improve the electrical coupling coefficient. Using electron microscopic analysis and field potential recordings, we examined here the use of gold micro-structures that mimic dendritic spines in their shape and dimensions to improve the adhesion and electrical coupling between neurons and micro-electronic devices. We found that neurons cultured on a gold-spine matrix, functionalized by a cysteine-terminated peptide with a number of RGD repeats, readily engulf the spines, forming tight apposition. The recorded field potentials of cultured Aplysia neurons are significantly larger using gold-spine electrodes in comparison with flat electrodes.

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“…Gold spines for electrical measurements were prepared on silicon wafers as described by Huys et al (2008). Briefly, the electrodes were patterned by the Cu-damascene method.…”

mentioning

confidence: 99%

Spine-shaped gold protrusions improve the adherence and electrical coupling of neurons with the surface of micro-electronic devices

Hai

Dormann

Shappir

et al. 2009

J. R. Soc. Interface.

Self Cite

137

186

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“…This would eventually lead to a stronger electrical coupling because of a higher electrical resistance in the gap between cell and chip. Preliminary data suggest strong engulfment of the electrode by the cell membrane, as could be observed by immunohistochemical actin filament staining and focused ion beam scanning electron microscopy ( Figure 1) ; Huys et al (2008); Van Meerbergen et al (2008)). Moreover, the electrodes are spaced very close to each other, which allows for single cell recording and stimulation.…”

Section: Recent Advances In Multitransistor Arraysmentioning

confidence: 64%

New Trends and Challenges in the Development of Microfabricated Probes for Recording and Stimulating of Excitable Cells

Braeken¹,

Prodanov²

2010

New Developments in Biomedical Engineering

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types of neural prostheses are unclear. Notable exceptions for this are the auditory cochlear prostheses, which exploit the sonotopic organization of the cochlea, the cardiac pacemakers, the phrenic stimulator and the foot drop stimulator. Current DBS systems for Parkinson's disease rely on the high frequency stimulation, which can result in blocking of certain mid-brain pathways. The encoding of the kinematic information in these structures is still unknown. Dissociated cell cultures lose their anatomical connectivity and structure when seeded on substrates in vitro. The 'new' situation that exists on these surfaces is interesting in terms of communication between individual cells and their function in advanced networks. However, the translation of these processes to the in vivo situation is not always straightforward and challenging. On the level of the overall device several types of issues can be grouped in the categories of configuration and operation. Issues related to the configuration can be related to the assembly, the size and topology of the device, the encapsulation and the packaging of the device. Devices have to be packaged in a way that prevents leakage of the environment, which contains high salt and protein concentrations, to the chip. These packages have to be leakage-proof on the one hand and non-toxic for the cell culture or tissue on the other hand. Issues related to the operation of the device can be grouped under the power, safety and communication of the device. The operation has to be compatible with cell cultures or in vivo environments, which in this case has to be in agreement with certain regulations. One other important aspect of the safety of operation are the interactions with other equipment, for example MRI.

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“…An important aspect is the interaction between the electrode and the neural tissue: a closer interaction will entail a better focalisation. To obtain such effect, 3D mushroom or pillar electrodes have been used in Micro Electrode Arrays to improve the interaction between cultivated cells and the electronic device [3].…”

Section: Introductionmentioning

confidence: 99%

Protuberant Electrode Structures for New Retinal Implants

Losada

Rousseau

Grzeskowiak

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Focal stimulation of neural tissue is a challenge in retinal prosthetic devices in which the aim is to improve the spatial resolution of the stimulation and therefore increase the electrode density of the prosthetic devices. Our work intends to create a new implant able to enhance the focalisation of the stimulation signal through protuberant electrodes. These electrodes are micro fabricated on a soft polyimide substrate using classical metal electrodeposition techniques. Before proceeding with fabrication a FEM model of the electrode's current density was done to select the best-performing structures and geometries in terms of local stimulation. Based on these models, several prototypes were fabricated and implanted in vivo into a rat's eye to verify the adaptation to the retina tissue.

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Novel concepts for improved communication between nerve cells and silicon electronic devices

Cited by 20 publications

References 17 publications

Spine-shaped gold protrusions improve the adherence and electrical coupling of neurons with the surface of micro-electronic devices

Spine-shaped gold protrusions improve the adherence and electrical coupling of neurons with the surface of micro-electronic devices

New Trends and Challenges in the Development of Microfabricated Probes for Recording and Stimulating of Excitable Cells

Protuberant Electrode Structures for New Retinal Implants

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