Novel detection method to rapidly quantify toxic cucurbitacin in Lagenaria siceraria (bottle gourd)

Saurabh, Chaturbhuj K.; Ghosh, Sunil K.; Sanyal, Bhaskar

doi:10.1007/s13197-022-05600-3

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Cucurbitacins are a group of triterpenoids that occur exclusively in most cucurbit crops, including bottle gourd [6,[30][31][32][33]44,55]. These compounds possess various human health benefits, including anti-cancer and anti-diabetic properties [1,6,7,9]. Evidence suggests drought tolerance in cucurbits could be linked to the accumulation of cucurbitacins [41].…”

Section: Discussionmentioning

confidence: 99%

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Cucurbitacins B, E and I Concentrations and Relationship with Drought Tolerance in Bottle Gourd [Lagenaria siceraria (Molina) Standl.]

Mkhize,

Shimelis,

Mashilo

2023

Plants

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Bottle gourd [Lagenaria siceraria (Molina) Standl.]) is a relatively drought-tolerant cucurbit due to the high composition of unique biochemical compositions, including cucurbitacin. The objective of this study was to determine the concentrations of cucurbitacins in bottle gourd and their relationship to drought tolerance. The study assessed 12 bottle gourd accessions grown under two moisture levels (i.e., non-stressed (NS) and drought-stressed (DS)) and three drought stress intensities (i.e., mild, moderate, and severe) using a 12 × 2 × 3 factorial experiment designed in a randomized complete block design with three replications. Control studies were undertaken under glasshouse conditions. The content of cucurbitacins B, E, and I were quantified in leaves and roots using high-performance liquid Cchromatography–mass spectrometry (HPLC-MS). The free radical scavenging activities of pure cucurbitacins B, E, and I were quantified using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and a ferrulic acid power assay (FRAP). Results revealed that cucurbitacins B and I were present in accessions BG-48, BG-58, BG-70, BG-78, BG-79, BG-81, BG-52, and GC in leaves and roots under DS condition. The contents of cucurbitacins B and I were enhanced under increased drought intensity for accessions BG-48, BG-81, and GC. In all the leaf and root samples, cucurbitacin E was not detectable. Based on the DPPH test, pure cucurbitacins I, B, and E reduced free radicals at maximum values of 78, 60, and 66%, respectively. Based on the FRAP assay, pure cucurbitacins I, B, and E had maximum ferric-reducing powers of 67, 62, and 48%. Additionally, cucurbitacin I recorded the highest antioxidant activity compared to cucurbitacins B and E. Increased cucurbitacin accumulation and antioxidant properties indicate their role in minimising cell damage caused by oxidative stress under drought-stressed environments. The present study revealed that cucurbitacins B and I serve as novel biochemical markers for screening drought tolerance in bottle gourd or related cucurbits.

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Section: Discussionmentioning

confidence: 99%

“…Bottle gourd fruits are harvested while tender and boiled until soft for consumption. They are a source of essential nutrients, including iron, zinc, nitrogen, manganese, copper, phosphorus, potassium, calcium, and magnesium [3][4][5][6]. Sugar and milk are often added to the boiled fruit to enhance taste [7].…”

Section: Introductionmentioning

confidence: 99%

Cucurbitacins B, E and I Concentrations and Relationship with Drought Tolerance in Bottle Gourd [Lagenaria siceraria (Molina) Standl.]

Mkhize,

Shimelis,

Mashilo

2023

Plants

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“…The fruits are also a good source of essential amino acids, including aspartic acid, threonine, serine, glutamic acid, alanine, valine, phenylalanine, lysine and arginine, and phytochemical compounds, including phenolics, flavonoids and terpenoids [9,10]. Wild fruits of the crop are bitter due to the presence of cucurbitacins, which possess pharmaceutical values including anti-cancer and anti-diabetes [10][11][12]. The leaves are a good source of essential nutrients such as zinc, nitrogen, manganese, and copper [8].…”

Section: Introductionmentioning

confidence: 99%

Combining Ability and Heterosis among Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Selections for Yield and Related Traits under Drought-Stressed and Non-Stressed Conditions

2023

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Bottle gourd [Lagenaria siceraria (Molina) Standl.] is cultivated for multiple utilities, including as a leafy vegetable, for fresh and dried fruits and seeds. It is an under-researched and -utilized crop, and modern varieties are yet to be developed and deployed in sub-Saharan Africa (SSA). There is a dire need for pre-breeding and breeding of bottle gourds for commercialization in SSA. Therefore, this study aimed to determine the combining ability and heterosis among selected genotypes of bottle gourd for fruit yield and related traits under drought-stressed and non-stressed conditions to select the best parents and hybrids. Eight preliminarily selected and contrasting parents with drought tolerance were crossed using a half-diallel mating design. The 8 parents and 28 crosses were evaluated under non-stressed (NS) and drought-stressed (DS) conditions across two growing seasons (2020/21 and 2021/22) using a 6 × 6 alpha lattice design with three replicates. Data were collected on fruit yield and related traits and subjected to analysis of variance, combining ability and heterosis analyses. Significant (p < 0.05) specific combining ability (SCA) and general combining ability (GCA) effects were computed for fruit yield per plant (FYPP). The SCA × environment and GCA × environment interaction effects were highly significant (p < 0.001) for FYPP and SYPP. The results suggest that genetic effects were affected by the test environment. Parental genotypes BG-58 and GC recorded positive and significant GCA effects for FYPP under the DS condition, whereas GC recorded positive and significant GCA effects for FYPP under the NS condition. The two genotypes are ideal breeding parents for population development to select genotypes with high fruit and seed yields. Crosses BG-27 × BG-79, BG-79 × BG-52, BG-79 × BG-70, BG-80 × BG-70, BG-80 × GC, and BG-70 × GC recorded high and positive SCA effects for FYPP and SYPP under DS condition. Crosses BG-81 × BG-52, BG-81 × GC, BG-27 × BG-79, BG-27 × GC, BG-79 × GC, BG-80 × BG-70, BG-81 × BG-58, BG-27 × BG-80, BG-27 × BG-58, BG-79 × BG-52, BG-52 × BG-58, BG-80 × BG-58, and BG-58 × BG-70 recorded high and positive SCA effects for FYPP and SYPP under NS condition. Crosses BG-80 × BG-58, BG-27 × BG-79, BG-79 × BG-52, BG-27 × BG-52, and BG-52 × BG-80 showed high and positive mid- and better-parent heterosis under DS condition for FYPP and SYPP. Crosses BG-27 × GC, BG-79 × GC, BG-27 × BG-58, and BG-27 × BG-79 showed high and positive mid- and better parent heterosis under NS condition for FYPP and SYPP. The newly selected families are recommended for multi-environment evaluation forrelease and commercialization in South Africa or similar agroecologies.

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Hybrid performance of bottle gourd [Lagenaria siceraria] under drought stress and non-stress conditions

Mkhize,

Shimelis,

Mashilo

2025

Ecological Genetics and Genomics

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Novel detection method to rapidly quantify toxic cucurbitacin in Lagenaria siceraria (bottle gourd)

Cited by 3 publications

References 29 publications

Cucurbitacins B, E and I Concentrations and Relationship with Drought Tolerance in Bottle Gourd [Lagenaria siceraria (Molina) Standl.]

Cucurbitacins B, E and I Concentrations and Relationship with Drought Tolerance in Bottle Gourd [Lagenaria siceraria (Molina) Standl.]

Combining Ability and Heterosis among Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Selections for Yield and Related Traits under Drought-Stressed and Non-Stressed Conditions

Hybrid performance of bottle gourd [Lagenaria siceraria] under drought stress and non-stress conditions

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