Novel diagnostic method of testing for <i>Helicobacter pylori</i> infection using the rapid leukocyte strip test, Leukostix

Matsuda, Mitsuru; Noda, Yoichi; Takemori, Yasuhiro

doi:10.1046/j.1440-1746.2003.03150.x

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…In fact, the semi-quantitative rapid strip test was originally designed to recognize the presence of polymorphonuclear leukocytes and other abnormalities associated with infection in urine through the detection of enzyme leukocyte esterase [26]. Beyond its utility for urinary tract infection screening, the leukocyte esterase test has been evaluated, achieving variable results and reliability, for a great array of bacterial inflammatory diseases, including meningitis, peritonitis, peritoneal lavage in abdominal trauma, Helicobacter pylori in gastric mucosa, inflammatory synovial fluid, and vaginitis [26][27][28][29][30][31][32][33][34][35]. In addition, the urine strip test was developed for the analysis of nine different parameters, not all of which, however, are present in saliva.…”

Section: Discussionmentioning

confidence: 99%

Oral Diagnostic Methods for the Detection of Periodontal Disease

et al. 2021

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Periodontitis is a common immune-inflammatory oral disease. Early detection plays an important role in its prevention and progression. Saliva is a reliable medium that mirrors periodontal health and is easily obtainable for identifying periodontal biomarkers in point-of-care diagnostics. The aim of this study is to evaluate the effectiveness of diagnostic salivary tests to determine periodontal status. Whole saliva (stimulated/unstimulated) from twenty healthy and twenty stage III grade B generalized periodontitis patients was tested for lactoferrin, alkaline phosphatase, calcium, density, osmolarity, pH, phosphate, buffer capacity, salivary flow rate and dynamic viscosity. A semi-quantitative urinary strip test was used to evaluate markers of inflammation in saliva (erythrocytes, leukocytes, urobilinogen, nitrite, glucose, bilirubin, and ketones), clinical periodontal parameters and pathogenic bacteria. Concentrations of lactoferrin, hemoglobin, and leukocytes were found to be significantly higher in the stimulated and unstimulated saliva in periodontitis patients compared to healthy patients, whereas alkaline phosphatase levels were higher in unstimulated saliva of periodontitis patients (p < 0.05). Periodontal biomarker analysis using test strips may be considered rapid and easy tool for distinguishing between periodontitis and healthy patients. The increase in lactoferrin, hemoglobin, and leucocytes—determined by strip tests—may provide a non-invasive method of periodontal diagnosis.

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Section: Discussionmentioning

confidence: 99%

Oral Diagnostic Methods for the Detection of Periodontal Disease

et al. 2021

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“…This is an original and simple approach to detect polymorphs quantitatively in gastric mucosa. The accuracy was excellent notably after eradication and there was a good correlation with the bacterial mass [1]. A breakthrough may come in the future from endoscopy.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Helicobacter pylori Infection

et al. 2004

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While there are some attempts to improve culture of Helicobacter pylori, molecular methods have been the main focus of this interest. Their main application concerns the development of rapid tests also allowing the determination of bacterial resistance, i.e. real-time polymerase chain reaction (PCR) or fluorescence in situ hybridization (FISH), or to genotype the strains. Attempts to improve, simplify or explain the discrepancies of urea breath test results have been made and new generation of stool antigen test with monoclonal antibodies either using the standard ELISA format or rapid immunoenzymatic detection have confirmed their value. With regard to serology, studies have mainly focused on the distinction of infections with more pathogenic strains and the ability to diagnose atrophic gastritis with the Gastropanel.

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“…Urine reagent strip tests have been implemented for testing body fluids with varying results. [11][12][13][14][15][16][17][18][19][20][21][22][23] To our knowledge, they have not yet been evaluated for testing the quality of sputum samples before bacteriologic processing. The present prospective study demonstrated that the rapid strip could be used as a rapid method for evaluating the quality of sputum as informative or uninformative using the SG reagent.…”

Section: Discussionmentioning

confidence: 99%

“…Beyond its utility for urinary tract infection screening, the LE test has been evaluated for the diagnosis of bacterial meningitis and peritonitis, [11][12][13][14][15][16] peritoneal lavage in abdominal trauma, 17 the presence of Helicobacter pylori in gastric mucosa, 18 inflammatory synovial fluid, 19 and vaginitis, 20 with variable results of reliability.…”

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confidence: 99%