Novel electrochemical aptamer biosensor based on gold nanoparticles signal amplification for the detection of carcinoembryonic antigen

Shu, Huawei; Wen, Wei; Xiong, Huayu; Zhang, Xun

doi:10.1016/j.elecom.2013.09.018

Cited by 100 publications

(34 citation statements)

References 21 publications

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“…The Ab 2 -Fc-AuNPs conjugates were prepared according to previous reports with some modifications [25]. The pH value of 500 μL AuNPs colloids was adjusted to 9.0 by 0.1 M K 2 CO 3 .…”

Section: Preparation Of Ab 2 -Fc-aunps Conjugatesmentioning

confidence: 99%

Electrochemical immunosensor for the prostate specific antigen detection based on carbon nanotube and gold nanoparticle amplification strategy

et al. 2015

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We describe a highly sensitive electrochemical sandwich-type immunosensor for the prostate specific antigen (PSA), a biomarker related to prostate cancer. A glassy carbon electrode was covalently modified with multiwalled carbon nanotubes to which the anti-PSA was immobilized. Following addition of a sample containing PSA, a secondary antibody is added that consists of gold nanoparticles modified with secondary antibody and 6-ferrocenyl hexanethiol acting as the signal molecule. This approach represents a multiple signal amplification strategy in that the functionalized carbon nanotubes improve the electron transfer on the surface of the electrode, while the gold nanoparticles act as carriers for capturing large quantities of Ab 2 and ferrocene. The modifying processes were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under the optimal conditions, the differential pulse voltammetric signals (acquired at a typical potential of 0.31 V vs. SCE) is linearly related to the concentration of PSA in the 10 pg.mL −1 to 100 ng.mL −1 range. The detection limit is 5.4 pg.mL −1 . When applied to the determination of PSA in spiked human serum, the recoveries were between 92.4 and 120.0 %. We perceive that this immunosensor holds great promise in the field of clinical screening for cancer biomarkers.

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“…The Ab 2 -Fc-AuNPs conjugates were prepared according to previous reports with some modifications [25]. The pH value of 500 μL AuNPs colloids was adjusted to 9.0 by 0.1 M K 2 CO 3 .…”

Section: Preparation Of Ab 2 -Fc-aunps Conjugatesmentioning

confidence: 99%

Electrochemical immunosensor for the prostate specific antigen detection based on carbon nanotube and gold nanoparticle amplification strategy

et al. 2015

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“…After termination of the reaction by strong acid, yellow compounds are produced and the color change is very easy to identify, providing G‐quadruplex–hemin DNAzyme the potential for colorimetric assays . More interestingly, two aptamers of CEA (Apt1 and Apt2) have been selected and the second aptamer (Apt2) is the G‐rich DNA sequence, which is used to make G‐quadruplex–hemin DNAzyme successfully in our work. In addition, magnetic beads modified by amino, carboxyl, and streptomycin are widely used to separate targets efficiently from serum in clinical tests .…”

Section: Introductionmentioning

confidence: 97%

An Aptamer‐based Self‐Catalytic Colorimetric Assay for Carcinoembryonic Antigen

Zou

Wang

2017

Bulletin Korean Chem Soc

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We report a visualization assay for carcinoembryonic antigen (CEA). The assay was prepared based on target‐specific recognition, aptamer‐based DNAzyme self‐catalysis, and colorimetric reaction. Aptamer of CEA was gathered at the surface of magnetic beads for sandwiched co‐conjugate when CEA was added. The second aptamer (Apt2) on the beads was folded into G‐quadruplex by KCl to make G‐quadruplex‐hemin DNAzyme with hemin. Apt2‐based DNAzyme catalyzed H2O2‐mediated oxidation of 3,3′,5,5′‐tetramethylbenzidine, resulting in color changes and significant increase in absorption intensity at 450 nm (ΔA450nm). Yellow products can be observed with naked eyes when over 1.0 ng/mL CEA was added and there was a linear correlation between ΔA450 nm and CEA concentrations in the range of 0.1–8.0 ng/mL with the detection limit of 0.06 ng/mL. The proposed method was then used to determine CEA in human serum from healthy people and tumor patients, revealing a correlation with data from the electrochemiluminescent immunoassay. Owing to its simplicity, selectivity, and low‐cost, this method is a promising candidate for detecting CEA and clinical diagnosis of cancer.

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“…A concentration of CEA as low as 0.5 ng/mL can be detected, and this sensor showed successful results in human serum. 21 AuNPs were also conjugated with polyclonal antibodies and added on the polydiacetylene liposomes to enhance fluorescence signals. As shown in Figure 2, Won and Sim 22 demonstrated about 100-fold increase in sensitivity by employing AuNPs to detect human immunoglobulin E (hIgE).…”

Section: Aunp-based Biosensorsmentioning

confidence: 99%

“…20 As shown in Figure 1, carcinoembryonic antigens (CEAs) were also detected by using two oligonucleotide aptamers. 21 Firstly, aptamer-1 (thiol-terminated CEA aptamer 1) was immobilized onto the bare gold electrode. The electrode was then immersed in CEA-containing solution, followed by the addition of 6-ferrocenyl hexanethiol (Fc)-capped AuNPs-aptamer-2 conjugate.…”

Section: Aunp-based Biosensorsmentioning

confidence: 99%

Nanomaterial-based biosensors for biological detections

Su¹,

Li²,

Jin³

et al. 2017

AHCT

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Nanomaterial-based biosensors have become one of the major topics in the field of diagnostics. With the growing demand on devices with improved sensitivity and selectivity, rapid response time, and low cost, four categories of nanomaterials have become popular in biosensor research: gold nanoparticles, graphene, carbon nanotubes, and photonic crystals. The ongoing research has brought new designs of biosensors based on nanomaterials, which have greatly improved the potential of field-deployable microfabricated devices. This review describes the recent technologies employing the aforementioned nanomaterials for electrochemical detection of biomolecules, including glucose, DNA, protein, toxins, and so on. We envisage that miniaturized lab-on-a-chip devices employing these nanomaterials will soon be an essential part of our daily life.

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Novel electrochemical aptamer biosensor based on gold nanoparticles signal amplification for the detection of carcinoembryonic antigen

Cited by 100 publications

References 21 publications

Electrochemical immunosensor for the prostate specific antigen detection based on carbon nanotube and gold nanoparticle amplification strategy

Electrochemical immunosensor for the prostate specific antigen detection based on carbon nanotube and gold nanoparticle amplification strategy

An Aptamer‐based Self‐Catalytic Colorimetric Assay for Carcinoembryonic Antigen

Nanomaterial-based biosensors for biological detections

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