Novel experimental model of non-infectious pharyngitis in rats

Viswanatha, Gollapalle Lakshminarayanashastry; Thippeswamy, Agadi Hiremath; Rafiq, Mohamed; Jagadeesh, M.; Baig, Mirza Rizwan; Suryakanth, D.A.; Azeemuddin, Mohammed; Patki, Pralhad Sadashiv; Ramakrishnan, Shyam

doi:10.1016/j.vascn.2013.12.001

Cited by 8 publications

(11 citation statements)

References 30 publications

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“…However, there is a lack of scientific evidence related to their beneficial effect with respect to non-infectious type of pharyngitis. Incidentally, there is a paucity of scientific literature and reports related to screening models for non-infectious type of pharyngitis, hence in our previous study we have standardized a novel experimental animal model for non-infectious type of pharyngitis in rat using pyridine as a inducer [10] . In the present study, we have evaluated KS (2 and 4 ml/kg, p.o.)…”

Section: Resultsmentioning

confidence: 99%

“…Recently a paper published by Bertold et al, stated that currently there is a lack rigorous trials (both preclinical and clinical) relevant to non-infectious pharyngitis and it is mainly due to lack of suitable preclinical animal model for non-infectious pharyngitis [5] . In this context, in our previous studies we have developed a novel experimental animal for non-infectious pharyngitis using various concentrations of pyridine in rats [10] .…”

Section: Discussionmentioning

confidence: 99%

“…In short, The tongue was slightly pulled out and pharynx area was opened deep into the oral cavity with the help of blunt forceps and the pyridine was applied with the help of cotton swab, gently for 5 s at each time point, for three times (approximately 50 μl). For G-I saline solution was applied similarly, since the pyridine solution was prepared in saline [10] .…”

Section: Methodsmentioning

confidence: 99%

“…However, currently there is a lack of rigorous trials (both preclinical and clinical) for the treatment of non-infectious pharyngitis, one of the important factor hampering the efforts in identifying the effective new treatments is the lack of a suitable animal model for non-infectious pharyngitis [5] . In this context, we have developed a novel animal model for non-infectious pharyngitis in rats using pyridine as an inducer [10] , it was found to be useful in screening the beneficial effect of synthetic, plant based medicines in treating non-infectious pharyngitis. In continuation, the present study was aimed to evaluate Koflet syrup, Koflet lozenges against pyridine-induced pharyngitis in rats.…”

Section: Introductionmentioning

confidence: 99%

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Ameliorative effect of Koflet formulations against pyridine-induced pharyngitis in rats

Viswanatha¹,

Rafiq²,

Thippeswamy³

et al. 2014

Toxicology Reports

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In present study two formulations of Koflet (syrup and lozenges) were evaluated against pyridine-induced pharyngitis in rats. Topical application of 10% pyridine showed extravasation of Evans blue stain as a characteristic feature of on-going inflammation. In addition, the levels of TNF-α (p < 0.01) and IL-6 (p < 0.01) were significantly increased compared to control. Further, histopathology of the pharyngeal tissue showed submucosal gland hypertrophy, severe mucosal inflammation characterized by presence of mononuclear cells and neutrophils along with haemorrhages and congestion; however, saline applied animals (normal control) showed normal cytoarchitecture of the pharynx. Interestingly, pre-treatment with dexamethasone (1 mg/kg, p.o.), Koflet lozenges (KL) (500 and 1000 mg/kg, p.o.) and Koflet syrup (KS) (2 and 4 ml/kg, p.o.) for 7 days showed significant and dose dependent protection by decreasing the EB dye extravasation, and serum levels of TNF-α and IL-6. In addition, histopathological findings have further supported the protective effect of Koflet formulations. These findings suggest that, both Koflet syrup and Koflet lozenges are highly effective in treating non-infectious type of pharyngitis. Among the two formulations KS was found to be more potent than KL, and possible mechanism of action thought to be mediating through inhibition of TNF-α and/or phospholipids–arachidonic acid pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ameliorative effect of Koflet formulations against pyridine-induced pharyngitis in rats

Viswanatha¹,

Rafiq²,

Thippeswamy³

et al. 2014

Toxicology Reports

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“…These neuropeptides possess strong proinflammatory effects in the airway tissues, leading to increased vascular permeability and plasma extravasation (23). Given its characteristics, the capsaicin-induced model may not, however, entirely mimic the human condition and the spectrum of possible interventions; thus the pyridine-induced pharyngitis model, which has recently been shown to be a simple and versatile novel animal model for screening drugs against non-infectious pharyngitis in rats, was selected (24).…”

Section: Effect Of Vbc-1814/7j a Poly-phytocompound On A Non-infectmentioning

confidence: 99%

Effect of VBC-1814/7J, a poly-phytocompound, on a non-infectious model of pharyngitis

Uemura¹,

Nagpal

Zerbinati

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Pharyngitis presents as an inflammation of the oropharynx, and clinical examination often shows evidence of nasopharyngitis. In numerous cases the condition occurs as a self-limiting illness of non-infectious aetiology, whose clinical management remains a matter for debate given the inappropriateness of antibiotics, the reported worsening following steroid use and the recent discouragement of the use of Chinese herbal medicine. The aim of the present study was thus to test VBC-1814/7J, a poly-phytocompound with known anti-inflammatory and immune-response enhancing properties, in an experimental model of non-infectious pharyngitis. Experimental non-infectious pharyngitis was induced by applying a pyridine solution to the surface of the pharyngeal mucosa in rats that were either normally fed (group A) or fed VBC-1814/7J three days prior to and three days subsequent to the induction of pharyngitis (group B). Healthy rats treated with topical saline were used as a control (group C). At time-points of 0, one hour, one day and three days sacrifices were carried out and microscopic examination, Evans blue (EB) dye extravasation and tissue concentrations of tumour necrosis factor (TNF)-α, interleukin (IL)-6 and mRNA of α-and β-defensins were studied. As compared with group C, group A showed significant microscopic damage, EB extravasation, and increases in the levels of TNF-α and IL-6, as well as in the mRNA of three defensins (P<0.001) on the third day of observation. VBC-1814/7J significantly mitigated these microscopic and inflammatory markers while allowing a prompter and wider defensin reaction (P<0.05 vs. group A). These data suggest that VBC-1814/7J, as demonstrated in earlier studies, has the potential to address non-infectious pharyngitis in clinical practice.

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Treatment of acute pharyngitis in rats with season tea decoctions from traditional Chinese medicine through a synergistic and subtle regulation of ARNTL and BHLHE40

Zhao,

Lu,

Yan

et al. 2025

Journal of Ethnopharmacology

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Novel experimental model of non-infectious pharyngitis in rats

Cited by 8 publications

References 30 publications

Ameliorative effect of Koflet formulations against pyridine-induced pharyngitis in rats

Ameliorative effect of Koflet formulations against pyridine-induced pharyngitis in rats

Effect of VBC-1814/7J, a poly-phytocompound, on a non-infectious model of pharyngitis

Treatment of acute pharyngitis in rats with season tea decoctions from traditional Chinese medicine through a synergistic and subtle regulation of ARNTL and BHLHE40

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