Novel Extract from Beetle Ulomoides dermestoides: A Study of Composition and Antioxidant Activity

Abstract

A biologically active extract from the darkling beetle Ulomoides dermestoides was obtained using the electro-pulse plasma dynamic extraction method. The beetle water extract contained a complex of antioxidant substances such as antioxidant enzymes and nonprotein antioxidants, as well as a complex of heat shock antistress proteins. This determines the rather high antioxidant activity of the aqueous extract of the beetle, i.e., 1 mg of dry matter/mL of the extract has an equivalent antioxidant activity to 0.2 mM Trolox (a water-soluble analog of vitamin E). It was shown that the beetle extract can lead to a 25–30% increase in the average lifespan of nematode Caenorhabditiselegans, under normal conditions, and a 12–17% increase under conditions of oxidative stress (with paraquat), and significantly inhibits the fructosylation reaction of serum albumin. Therefore, the beetle aqueous extract shows promise as a biologically active complex exhibiting antioxidant activity.

Nutritional, Physiochemical, and Biological Value of Muffins Enriched with Edible Insects Flour

“…Insects are a natural source of substances with biological activity, including antibacterial, antiviral, and anti-inflammatory activities [7]. In traditional medicine, some species of edible insects are used to treat inflammatory diseases [8,9]. Stull et al found that eating a breakfast enriched with 25 g of cricket powder for 2 weeks reduced circulating levels of the pro-inflammatory cytokine TNF-α in the plasma of 20 healthy adults [10].…”

Wound healing approach based on excretory-secretory product and lysate of liver flukes

“…The obtained gel fragments were washed 2 times for 15 min with 100 μL of 50 mM ammonium bicarbonate solution, then dried with 100% acetonitrile (15 min), after which the solution was removed, and the samples were dried on a centrifugal vacuum evaporator (Concentrator Plus, Eppendorf). Proteins in each gel fragment were distained and digested by trypsin using the protocol described previously 65 , 66 .…”

“…An isolation intensity threshold of 50,000 counts was determined for precursor’s selection and up to top 20 precursors were chosen for fragmentation with high-energy collisional dissociation (HCD) at 29 NCE. Precursors with a charge state of 1+ and more than 5+ were rejected and all measured precursors were dynamically excluded from triggering of a subsequent MS/MS for 90 s 65 .…”

“…Proteins were considered as significantly identified if at least two peptides were found for them. Label-free protein quantification was based on iBAQ 65 .…”

“…The LC-MS/MS of obtained peptides was perfomed by the method described below 65 . Proteomic analysis of peptides was carried out using an Ultimate 3000 RSLCnano chromatographic HPLC system (Thermo Scientific, USA) connected to a mass spectrometer Q-exactive HFX (Thermo Scientific, USA).…”