Novel fermentation media for production of δ-endotoxins from Bacillus thuringiensis

Salama, H. S.; Foda, M. S.; Dulmage, Howard T.; Sharaby, A.

doi:10.1016/0022-2011(83)90231-8

Cited by 57 publications

(22 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tirado-Montiel et al (2001) fi rst tested the use of wastewater sludge for biopesticide production, although the entomotoxicity level reported was low. Many authors report the use of by-products and raw materials to develop Bt based biopesticides (Salama et al 1983, Obeta & Okafor 1984, Morris et al 1997. Moreover, the low cost of by-products as nutrient source in fermentation media for Bt biopesticide production has received little attention.…”

mentioning

confidence: 99%

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Valicente¹,

Mourão²

2008

Neotrop. entomol.

View full text Add to dashboard Cite

mentioning

confidence: 99%

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Valicente¹,

Mourão²

2008

Neotrop. entomol.

View full text Add to dashboard Cite

“…Media containing fishmeal, and corn-steep liquor for production of B. thuringiensis were reported (Saalma 1983;Kumar et al 2000). Media containing molasses, corn extract, and mineral salts (Dregval et al 1999), corn steep liquor, glucose, and mineral salts (Luna 2004), glucose, corn extract, sodium humate, and mineral salts (Dregval et al 2002), or potato, common sugar, and Bengal gram (Cicer arietinum L.) (Poopathi and Kumar 2003) were all used for growth and production of B. thuringiensis.…”

Section: Introductionmentioning

confidence: 99%

Development of a Cost Effective Medium for Production of Bacillus Thuringiensis Bioinsecticide Using Food Barley

Shojaaddini

Moharramipour

Khodabandeh³

et al. 2010

Journal of Plant Protection Research

View full text Add to dashboard Cite

Genetically manipulated food barley, Hordeum vulgare L. var. valfajr, was compared to commercial-grade starch as carbon sources in groundnut and soybean based media for supporting growth, sporulation and delta-endotoxin production by Bacillus thuringiensis Berliner B. thuringiensis kurstaki HD-1 and a promising newly isolated B. thuringiensis strain (referred to as BTA) were used. MgCl 2 , CaCl 2 , and MnCl 2 were used as trace-elements. Culture media were compared in shaken flasks and then in 5 l Fermentors. Biomass, delta-endotoxin levels and the number of spores as colony forming units (CFU) were evaluated. For each of the two strains, biomass and delta-endotoxin synthesis were not significantly different in soybean-based media after substitution starch by barley flour. There were significant differences between the two strains with respect to biomass and toxin production. Evaluated costs of media preparation showed that food barley is an economical alternative to commercial-grade starch in the production of BTA bioinsecticide.

show abstract

“…Se han realizado ensayos con cinco medios de cultivo que contienen semillas de legumbres, sangre de vaca seca y sales minerales. A partir de estos nuevos medios de cultivo se ha obtenido una mayor producción de las toxinas del insecticida específicamente contra especies de Culex, Anopheles y Aedes (45). Otros medios producidos a partir de comida para peces, fríjol de soya y maíz han reportado eficiencia para la producción en masa (46)(47)(48), al igual que la utilización de papas en un medio de fermentación (49).…”

Section: Producción Industrialunclassified

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

Romero

Rodríguez

Ramírez

2009

nova

View full text Add to dashboard Cite

ResumenEl aumento en la incidencia de enfermedades de interés en salud pública como malaria, dengue y fiebre amarilla asociado a los efectos secundarios del uso de plaguicidas químicos y la resistencia generada, ha impulsado a la búsqueda de nuevas alternativas para la erradicación de sus vectores. Una opción viable como bacteria entomopatógena es el Bacillus sphaericus, descrita por primera vez en 1904 por Neide y Meyer, quienes descubrieron una bacteria formadora de esporas, que existe en la naturaleza y que crece fácilmente tanto in vitro como en el cadáver de las larvas.Su actividad larvicida se debe a la producción de una inclusión cristalina denominada toxina binaria A y B de 41,9 y 51,4 kDa, respectivamente, las cuales, al ser liberadas en el intestino de los insectos susceptibles en su fase larvaria y tras ser solubilizada por el pH alcalino en la porción media del intestino, activan las proteasas causando deshidratación y muerte a los vectores. Es considerada como un biolarvicida altamente potencial y eficaz para el control biológico de Anopheles spp., Aedes spp. y Culex spp. Comercialmente puede producirse por fermentación sumergida a pequeña escala con materiales proteínicos de desecho. Este producto ha sido utilizado y registrado en numerosos países de América Latina, donde se han obtenido buenos resultados después de su aplicación.Palabras clave: Bacillus sphaericus, biocontrolador, biolarvicida, toxina binaria Bin A y Bin B. Abstract Bacillus sphaericus: vector control strategies that produce malaria, yellow fever, and dengue The increased incidence of diseases of public health such as malaria, dengue and yellow fever associated to side effects of the use of chemical pesticides and the resistance generated has prompted the search for new alternatives for the elimination of its vectors. A viable option is the entomopathogenic bacteria Bacillus sphaericus, first described in 1904 by Neide and Meyer who discovered spore-forming bacteria that exists in nature and is easily grown in vitro and in the body of the larvae.Its larvicidal activity is due to the production of a crystalline inclusion, called binary toxin A and B of 41.9 and 51.4 kDa, respectively, which, when released in the gut of susceptible insects in their larval ARTÍCULO DE REVISIÓN

show abstract

Novel fermentation media for production of δ-endotoxins from Bacillus thuringiensis

Cited by 57 publications

References 8 publications

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Development of a Cost Effective Medium for Production of Bacillus Thuringiensis Bioinsecticide Using Food Barley

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

Contact Info

Product

Resources

About