Novel fermentation process strengthening strategy for production of propionic acid and vitamin B12 by Propionibacterium freudenreichii

Abstract

An efficient fermentation-strengthening approach was developed to improve the anaerobic production of propionic acid and vitamin B12 by co-fermentation with Propionibacterium freudenreichii. Vitamin B12 production from glucose resulted in relatively high productivity (0.35 mg/L h) but a low propionic acid yield (0.55 g/g). By contrast, glycerol gave a high propionic acid yield (0.63 g/g) but low productivity (0.16 g/L h). Co-fermentation of glycerol and glucose with a gradual addition strategy gave high yields (propionic acid: 0.71 g/g; vitamin B12: 0.72 mg/g) and productivities (propionic acid: 0.36 g/L h; vitamin B12: 0.36 mg/L h). Finally, the integrated feedstock and fermentation system strengthening strategy was demonstrated as an efficient method for the economic production of bio-based propionic acid and vitamin B12.

Research on the ability of propionic acid and vitamin B12 biosynthesis by Propionibacterium freudenreichii strain T82

“…Vitamin B12 production by P. freudenreichii T82 could also be improved by using more suitable carbon sources. Wang et al ( 2014 ) were able to produce high yields of vitamin B12 (0.72 mg/g) and propionic acid (0.81 g/g) in P. freudenreichii subsp. shermanii using concurrent glucose and glycerol fermentation.…”

EPS production by Propionibacterium freudenreichii facilitates its immobilization for propionic acid production

“…; Wang et al . ; Dishisha et al . ) and is used as probiotic agent, since it has generally regarded as safe status and is capable of surviving in the digestive tract (Jan et al .…”

Bio-produced Propionic Acid: A Review

“…To optimize the fermentation performance, a strategy of gradually adding feedstock was employed, in which glycerol was introduced at set times (12,24,48, and 72 h) with a constant mass ratio of 2.5 to glucose. When glycerol was added after 48 h, the highest product concentration (propionic acid: 42.7 g*L -1 ), the highest yield (propionic acid: 0.71 g*g -1 ), and highest productivity (propionic acid: 0.36 g*L -1 *h -1 ) of this experiment were obtained [46]. …”

“…Propionibacterium freudenreichii could also achieve a high propionic acid yield of 0.71 g*g -1 [46]. In this report batch fermentations were studied in three parallel 200 mL expanded bed adsorption bioreactors (EBAB) connected with a recirculation loop to a 5 L stirred-tank fermenter to control the temperature and pH.…”