Novel Fluorometric Assay for Hydroxyl Radical Scavenging Capacity (HOSC) Estimation

Moore, Jeffrey C.; Yin, Jun‐Jie; Yu, Liangli

doi:10.1021/jf052555p

Cited by 136 publications

(137 citation statements)

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“…Therefore, · OH was suggested to be an excellent target to investigate antioxidants in the fields of food, nutrition, health, and medicines. 21,22) In addition, ESR is widely used as a prerequisite tool to study free radicals even with suspensions or pastes, 23) and is thought to be useful to study slightly insoluble less-polar ginsenosides or MRPs. Especially, some triterpenoid glycosides and MRPs are known to have transition metal chelating and reducing capabilities closely related to · OH scavenging activity.…”

Section: Resultsmentioning

confidence: 99%

Study on the Hydroxyl Radical Scavenging Activity Changes of Ginseng and Ginsenoside-Rb2 by Heat Processing

Kang

Kim

Baek

et al. 2007

Biological & Pharmaceutical Bulletin

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Traditional herbal medicines have been processed to enhance their therapeutic effects, remove or reduce toxicity and side effects, and facilitate preparation and storage. In addition, the increased biological activities have been thought to be closely related to the compounds which are modified through processing.1-3) Therefore, there has been a growing interest in the bioactive constituents of herbal drugs modified by processing. [4][5][6] The root of ginseng, Panax ginseng C.A. MEYER (Araliaceae), has been heat processed to make white ginseng (WG, roots dried after peeling) and red ginseng (RG, steamed at 98-100°C and dried ginseng roots without peeling) for consumption. Especially, RG is more common as a functional food than WG in Asian countries, because steaming induces a change in the chemical constituents and enhances the biological activities of ginseng. [7][8][9][10] Recently, a method to increase the RG-specific ginsenosides by steaming WG at a higher temperature than RG was developed.2,11) This heat processed ginseng is termed sun ginseng (SG), and we have been investigating its enhanced free radical scavenging activity compared to conventional ginsengs and its active constituents.Phenolic compounds and maltol were responsible for the increased free radical scavenging activities of SG. In addition, Maillard reaction products (MRPs) were also thought to be related to the increased antioxidant activity of ginseng by heat processing, 5,6) because the Maillard reaction has been thought to be the major source correlated with increased efficacy by heat processing in various crude drugs or foods. 12,13) On the other hand, ginsenoside is one of the easily changeable components of ginseng by heat processing, 2,14) but heat processing-induced chemical and activity changes of ginsenosides considering the Maillard reaction have not yet been fully elucidated. In addition, the contents of less-polar ginsenosides Rg 3 , Rk 1 , Rg 5 , and maltol are known to increase by heat processing, and these compounds are supposed to be produced by the deglycosylation of diol-type ginsenosides and the Maillard reaction in ginseng by steaming. 6,14) Therefore, there is a need to investigate the structure and activity changes of isolated ginsenosides by heat processing with amino acids.In this study, we investigated the hydroxyl radical (· OH) scavenging activity changes of ginsengs and ginsenoside-Rb 2 (Rb 2 ) by heat processing using an electron spin resonance spectrometer (ESR). No report has compared the · OH scavenging activities of WG, RG, and SG using ESR, and ESR is thought as a versatile tool to detect free radicals even with slightly insoluble ginsenosides in suspension. In addition, Rb 2 , a well known diol-type triterpene glycoside that exists abundantly in Panax ginseng, was steamed with glycine, a frequently used amino acid in the Maillard reaction model system, 15) and the chemical and · OH scavenging activity changes were analyzed and compared. Shillim-Dong, Kwanak-gu, Seoul 151-742, Korea. Received October 7, 20...

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Section: Resultsmentioning

confidence: 99%

Study on the Hydroxyl Radical Scavenging Activity Changes of Ginseng and Ginsenoside-Rb2 by Heat Processing

Kang

Kim

Baek

et al. 2007

Biological & Pharmaceutical Bulletin

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“…Hydroxyl radical scavenging capacities were investigated using a Victor 3 multilabel plate reader (PerkinElmer) according to a laboratory protocol described earlier [12]. Hydroxyl radicals were generated by a Fenton-like reaction using H 2 O 2 and Fe 3+ , and trolox was used to prepare the standard curve.…”

Section: Hydroxyl Radical Scavenging Capacitymentioning

confidence: 99%

Fatty Acid Composition, Antioxidant Properties, and Antiproliferative Capacity of Selected Cold‐Pressed Seed Flours

Parry

Cheng

Moore

et al. 2008

J Americ Oil Chem Soc

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Cold-pressed seed flours from pumpkin, parsley, mullein, cardamom, and milk thistle were examined for total oil, fatty acid profile of the oil, total phenolic content (TPC), scavenging activities against peroxyl (ORAC), hydroxyl (HOSC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (RDSC) radicals, and antiproliferative capacity against HT-29 human colon cancer cells. The cold-pressed parsley seed flour contained a very high concentration of total oil-17.6 g/100 g flour-with primarily C18:1 fatty acid at 86.2 g/100 g fatty acids. All other flour oils had relatively high levels of saturated fats, ranging from 39.0 to 62.9 g/100 g fatty acids. The tested seed flours demonstrated significant TPC and free radical scavenging activities. Milk thistle seed flour had the highest TPC value of 25.2 mg gallic acid equivalent per g flour (GAE mg/g) followed by that of parsley seed flour at 8.1 GAE mg/g. Milk thistle seed-flour extract also had significantly higher antioxidant activities than all other extracts against all tested radicals. The milk thistle seedflour extract had an ORAC value of 1131 lmol trolox equivalents (TE) per g flour (TE lmol/g), a HOSC value of 893 TE lmol/g, and an RDSC value of 61 TE lmol/g. Also, ORAC, HOSC, and TPC values were significantly correlated (P \ 0.01) under the experimental conditions. The cold-pressed milk thistle seed flour inhibited the proliferation of HT-29 cancer cells in a dose-dependent manner. Results from this study suggest that these coldpressed seed flours may serve as natural sources of antioxidants and may be used to improve human health.

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“…For example, Moore et al 43 noticed that randomly-methylated β-cyclodextrin could reduce the sensitivity of hydroxyl radical scavenging capacity (HOSC) measurements, since their experimental findings indicated the possibility of the • OH quenching of Me-β-CD. 43 García-Padial et al 44 pointed out that cyclodextrins may yield high blank values in the ORAC-FL antioxidant activity assay, and that intermolecular H-bonding between the cyclodextrin and the antioxidant it includes may attenuate the measured antioxidant activity. 44 It was found in this work that the complexed BC with β-CDs was a less effective antioxidant than free BC, considering the TEAC value of β-carotene in acetonated medium previously given as 3.10.…”

Section: Antioxidant Capacity Of Inclusion Complexesmentioning

confidence: 99%

Modified Radical Scavenging and Antioxidant Activity Measurement of ²-Carotene with ²-Cyclodextrins Complexation in Aqueous Medium

et al. 2017

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In order to evaluate the antioxidant capacity/activity of β-carotene (BC) in aqueous media, we investigated the inclusion complexes of BC with methyl-β-cyclodextrin (Me-β-CD), 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and 2-hydroxyethyl-β-cyclodextrin (HE-β-CD) that enhance water solubility and chemical stability. The inclusion complexes (monitored by FTIR) exhibited higher solubility than free BC, and phase solubility studies showed a linear increase in the solubility with the Me-β-CD concentration. Cupric ion-reducing antioxidant capacity (CUPRAC), ABTS-persulfate, peroxyl and hydroxyl radical scavenging assays were applied. CD-complexed β-carotene exhibited less effective antioxidative and radical scavenging than free BC dissolved in acetone. β-Carotene showed the highest antioxidant capacity in the presence of HE-β-CD, and the lowest with Me-β-CD, probably due to the deeper and more hydrophobic cavity of the latter. We believe that this is the first report on devising simple spectrophotometric methods for the wholistic assessment of antioxidant activity/capacity, hydroxyl and peroxyl radical scavenging activity of β-carotene in aqueous solution with CDs.

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Novel Fluorometric Assay for Hydroxyl Radical Scavenging Capacity (HOSC) Estimation

Cited by 136 publications

References 59 publications

Study on the Hydroxyl Radical Scavenging Activity Changes of Ginseng and Ginsenoside-Rb2 by Heat Processing

Study on the Hydroxyl Radical Scavenging Activity Changes of Ginseng and Ginsenoside-Rb2 by Heat Processing

Fatty Acid Composition, Antioxidant Properties, and Antiproliferative Capacity of Selected Cold‐Pressed Seed Flours

Modified Radical Scavenging and Antioxidant Activity Measurement of ²-Carotene with ²-Cyclodextrins Complexation in Aqueous Medium

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