Novel function of biguanides in inhibition of phospholipase D1 expression via a translational mechanism in cancer cells

Yao, Yuanfa; Li, Hanbing; Wang, Xinyi; Sun, Yuanhai; Zhao, Xuqin; Zha, Weiwei; Zhou, Jian; Toomre, Derek; Fu, Junfen; Xu, Yingke

doi:10.1016/j.gendis.2023.01.007

Cited by 2 publications

(3 citation statements)

References 5 publications

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“…Given that the basal activities of endogenous PLDs were typically low, their activation requires additional stimulation 23,24,29 , such as G-protein-coupled receptor agonists and PKC activation. To assess the PA-detecting performance of our enhanced biosensor in the cytosol, we generated a tetracycline-inducible construct of PLD with elevated basal activity (referred to here as PLD PMF100× ).…”

Section: Resultsmentioning

confidence: 99%

“…In previous reports and reviews, we highlighted the significant roles of PLDs and PA in cells 26,33,34 , especially in cancer cells. However, for a more comprehensive understanding of the diverse biological functions of various PLD isoforms and their real-time activity or dynamics, it requires the use of detection methodologies with enhanced performance.…”

Section: Discussionmentioning

confidence: 97%

“…In previous reports and reviews, we highlighted the significant roles of PLDs and PA in cells [22][23][24] , especially in cancer cells. However, to gain a comprehensive understanding of their spatiotemporal regulatory roles, we need to monitor their real-time activity and thus advanced detection methods are indispensable.…”

Section: Designing Optogenetics-based Biosensors With Circularly Perm...mentioning

confidence: 97%

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Optogenetic strategies for optimizing the performance of biosensors of membrane phospholipids in live cells

Yao,

Lou,

Jin

et al. 2023

Preprint

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High-performance biosensors are crucial for elucidating the spatiotemporal regulatory roles and dynamics of membrane lipids, but there is a lack of improvement strategies for biosensors with low sensitivity and low-content substrates detection. Here we developed universal optogenetic strategies to improve a set of membrane biosensors by trapping them into specific region and further reducing the background signal, or by optically-controlled phase separation for membrane lipids detection and tracking. These improved biosensors were superior to typical tools and light simulation would enhance their detection performance and resolution, which might contribute to the design and optimization of other biosensors.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Designing Optogenetics-based Biosensors With Circularly Perm...mentioning

confidence: 97%

See 1 more Smart Citation

Optogenetic strategies for optimizing the performance of biosensors of membrane phospholipids in live cells

Yao,

Lou,

Jin

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

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Optogenetic Strategies for Optimizing the Performance of Phospholipids Biosensors

Yao,

Lou,

Jin

et al. 2024

Advanced Science

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High‐performance biosensors play a crucial role in elucidating the intricate spatiotemporal regulatory roles and dynamics of membrane phospholipids. However, enhancing the sensitivity and imaging performance remains a significant challenge. Here, optogenetic‐based strategies are presented to optimize phospholipid biosensors. These strategies involves presequestering unbound biosensors in the cell nucleus and regulating their cytosolic levels with blue light to minimize background signal interference in phospholipid detection, particularly under conditions of high expression levels of biosensor. Furthermore, optically controlled phase separation and the SunTag system are employed to generate punctate probes for substrate detection, thereby amplifying biosensor signals and enhancing visualization of the detection process. These improved phospholipid biosensors hold great potential for enhancing the understanding of the spatiotemporal dynamics and regulatory roles of membrane lipids in live cells and the methodological insights in this study might be valuable for developing other high‐performance biosensors.

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Novel function of biguanides in inhibition of phospholipase D1 expression via a translational mechanism in cancer cells

Cited by 2 publications

References 5 publications

Optogenetic strategies for optimizing the performance of biosensors of membrane phospholipids in live cells

Optogenetic strategies for optimizing the performance of biosensors of membrane phospholipids in live cells

Optogenetic Strategies for Optimizing the Performance of Phospholipids Biosensors

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