Novel immortalized human vocal fold epithelial cell line: In vitro tool for mucosal biology

Abstract

Located in the larynx, and responsible for voicing, vocal folds (VF) are a pair of tissue shelves whose mucosa consists of two main cell types; VF stratified squamous epithelial cells cover and protect the lamina propria where VF fibroblasts produce extracellular matrix. 1 Interactions between these two cell populations dictate the architecture and biological function of VF mucosa which, in turn, determine ease of phonation, vocal quality, susceptibility to disease, and tissue repair outcomes. Because of the importance of epithelial cell-fibroblast interactions in shaping VF architecture and behavior in health, disease, and healing, as well as the significant costs associated with vocal dysfunction or disorders, it is imperative to

Descriptive proteomics of paired human vocal fold and buccal mucosa tissue

“…Recently, Chen et al. published the establishment of immortalized human VF epithelial cell lines [ 11 ]. However, these are still not widely available for research purposes.…”

Effects of Electronic (E)‐cigarette Vapor and Cigarette Smoke in Cultured Vocal Fold Fibroblasts

“…However, we acknowledge that epithelial cells are the first cell type in the vocal folds to encounter EV and CS and critical modulators of vocal fold disease development 9 . An immortalized human vocal fold epithelial cell line is now established 55 and should be tested in the future using this established in vitro methodology. While exposing submerged cells to EVE and CSE to examine cytotoxicity and other parameters is a recognized technique, it is not as physiologically relevant as work involving organotypic in vitro models at ALI.…”

Construction and evaluation of the SV40-LT and hTERT overexpression MDCK cell lines

“…The establishment and study of immortalized cell lines have gained much attention since Jerry et al (1978) proposed a molecular model of cell immortalization [16] . Xia et al (2021) stably transfected a retrovirus containing hTERT into human vascular endothelial (HVFE) cells to establish an immortalized HVFE cell line that could last for more than 8 months [17] . Li et al (2021) used viral transfection to immortalize normal human lung pericytes with a lentiviral vector carrying the SV40-LT antigen, and transfected cells showed robust SV40-LT expression, sustained proliferative capacity, and signi cant telomerase activity [18] .…”