Novel isocratic RP-HPLC method for simultaneous estimation of Berberine and Aloe-emodin

Jain, Vandana; Tandel, Leena; Sonone, Revati

doi:10.5958/0974-360x.2021.00117.7

Cited by 5 publications

(1 citation statement)

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“…Various analytical techniques have been developed for the estimation of these compounds, either individually or in combination, from different sources. For instance, high-performance liquid chromatography (HPLC) and UFLC methods have been established for the estimation of Quercetin [16][17][18][19][20][21][22], whereas HPLC and HPTLC techniques have been used for the estimation of Berberine [23][24][25][26][27][28][29][30][31][32][33][34], alone or in combination. Similarly, HPLC methods have been utilized for the quantification of Phytosterols [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

LC method for the quantification of Quercetin, Berberine, and Phytosterol in lyophilized liposome

Bhavsar,

Jha,

Patel

et al. 2023

Separation Science Plus

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The objective of this study was to create a liquid chromatographic method that could accurately and simultaneously quantify the amount of Quercetin, Berberine, and Phytosterol present in lyophilized liposome used to treat metabolic syndrome. To achieve this goal, the team utilized a Shim‐pack GIST C8 column with a gradient elution of 0.1% (V/V) triethylamine buffer: acetonitrile at 30°C. They analyzed the phytoconstituents using an eluent flow rate of 1 mL/min and ultraviolet detection at 205 nm. The method was linear within a range of 25–77 μg/mL, which had excellent correlation coefficients (R2) of 0.9998, 0.9998, and 0.9996 for Quercetin, Berberine, and Phytosterol, respectively. The %RSD for precision and robustness of the method were also less than 2%, and the recovery values for Quercetin, Berberine, and Phytosterol were 99.5%, 100.5%, and 99.8%, respectively. The assay values of were found to be 100.2%, 101.6%, and 100.5% for Quercetin, Berberine, and Phytosterol, loaded lyophilized liposomes, respectively. As a result, this specific, accurate, and precise method can be employed for the routine analysis of Quercetin, Berberine, and Phytosterols in QC laboratories.

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