Novel method for extracting exosomes of hepatocellular carcinoma cells

Zhu, Lin; Qu, Xiuhua; Sun, Yulin; Yang, Qian; Zhao, Xiulan

doi:10.3748/wjg.v20.i21.6651

Cited by 40 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, the protein yields from the studies were surprisingly coherent; the average yields from the analyzed studies displayed similar outcomes seen in multiple EV isolation comparison studies with regards to the yield distribution as a function of isolation procedure (Fig. 4) (Tauro et al 2012;Van Deun et al 2014;Zhu et al 2014).…”

Section: Methods Of Quantificationsupporting

confidence: 59%

Systematic review of factors influencing extracellular vesicle yield from cell cultures

et al. 2015

View full text Add to dashboard Cite

The potential therapeutic utility of extracellular vesicles (EVs) has spawned an interest into a scalable production, where the quantity and purity of EV samples is sufficient for clinical applications. EVs can be isolated using several different protocols; however, these isolation protocols and the subsequent methods of quantifying the resulting EV yield have not been sufficiently standardized. Therefore, the possibility of comparing different studies with respect to these parameters is limited. In this review, we have presented factors that might influence the yield and function of EVs from cell culture supernatants. The methods of isolation, downstream quantification, and culture conditions of the EV producing cells have been discussed. In order to examine the inter-study coherency of EV yields, 259 studies were initially screened, and 46 studies were included for extensive downstream analysis of EV yields where information pertaining to the isolation protocols and quantification methods was obtained from each study. Several other factors influencing yield were compared, such as cell type producing EVs, cell confluence level, and cell stimulation. In conclusion, various factors may impact the resulting EV yield, including technical aspects such as EV isolation and quantification procedures, and biological aspects such as cell type and culture conditions. The reflections presented in this review might aid in future standardization of the workflow in EV research.

show abstract

Section: Methods Of Quantificationsupporting

confidence: 59%

Systematic review of factors influencing extracellular vesicle yield from cell cultures

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Some studies have indicated that there are no significant differences in exosome population isolated by the Exoquick protocol compared with ultracentrifugation methods. 13,14 characterization of isolated exosomes Morphological assessment of the exosomes was performed using transmission electron microscopy (TEM, Philips CM30 electron microscope, Eindhoven, Netherlands) at 80 kV. Briefly, the exosome preparation was fixed for 1 hour in 4% paraformaldehyde and washed once with PBS.…”

Section: Preparation Of Conditioned Medium and Exosomes Isolationmentioning

confidence: 99%

Exosome-mediated delivery of functionally active miRNA-142-3p inhibitor reduces tumorigenicity of breast cancer in vitro and in vivo

Naseri

Oskuee

Jaafari

et al. 2018

IJN

209

116

View full text Add to dashboard Cite

BackgroundExosomes, widely recognized natural nanovesicles, represent one of the recently discovered modes of intercellular communication due to their ability to transmit crucial cellular information that can be engineered to have robust delivery and targeting capacity. MiR-142-3p, one of the upregulated microRNAs (miRNAs) in many types of breast cancer, activates the canonical Wnt signaling pathway and transactivates the miR-150 expression, and results in the hyperproliferation of cancer cells in vitro and mammary glands in vivo.Materials and methodsIn this study, we exploited the exosomes isolated from bone marrow-derived mesenchymal stem cells (MSCs-Exo) to deliver LNA (locked nucleic acid)-modified anti-miR-142-3p oligonucleotides to suppress the expression level of miR-142-3p and miR-150 in 4T1 and TUBO breast cancer cell lines.ResultsThe in vitro results showed that the MSCs-Exo can efficiently deliver anti-miR-142-3p to reduce the miR-142-3p and miR-150 levels and increase the transcription of the regulatory target genes, APC and P2X7R. We also evaluated in vivo distribution of the MSCs-Exo in tumor-bearing mice. The in vivo result indicated that MSCs-Exo can penetrate the tumor site and are suitable nanovehicles to deliver the inhibitory oligonucleotides into the tumor tissues to downregulate the expression levels of miR-142-3p and miR-150.ConclusionWe showed that MSCs-derived exosomes could be used as a feasible nanovehicle to deliver drug molecules like LNA-anti-miR-142-3p in both in vitro and in vivo studies.

show abstract

“…19 times higher protein quantity in the isolated exosomal dispersion and is proven simpler and faster [34]. Yet no further experiments are performed to clarify if all the protein yields are truly of exosomal origin.…”

Section: Commercial Isolation Kitsmentioning

confidence: 99%

“…ExoQuick revealed higher protein quantity in the isolated exosomal dispersion, and has been proven simpler and faster [34]. Yet, it still remains to be clarified if the proteins are not of extraneous origin…”

Section: • Exoquickmentioning

confidence: 99%

Exosome Isolation: Is There an Optimal Method with Regard to Diagnosis or Treatment?

Kotmakçı¹,

Akbaba²

2017

Novel Implications of Exosomes in Diagnosis and Treatment of Cancer and Infectious Diseases

View full text Add to dashboard Cite

Extracellular vesicles (EV) gained considerable interest in recent years as both diagnostic tools and templates for therapeutic applications. EVs carry a number of cell-specific markers which gave researchers the opportunity of employing them as liquid biopsies causing no discomfort to patients. On the other hand, they are very exciting candidates for drug delivery due to their eobiotic origin, physicochemical and size characteristics. Isolation of EVs is performed by several strategies, having advantages and disatvantages over each other. As such, the method of EV isolation and in particular exosome isolation determines the quality and purity of obtained vesicles. In this chapter, extracellular vesicle isolation methods are evaluated with regard to their further use. Methods such as ultracentrifugation with different modifications, size exclusion chromatography, ultrafiltration, affinity and precipitation are compared with respect to the yield efficacy and purity of isolates. Furthermore, the advantages and disadvantages of different methods according to the purpose of use are revealed. Recent progress and remaining challenges in the isolation of EVs with regard to diagnosis and treatment is reviewed and discussed. In order to select the most suitable method researchers should clearly define purity, yield, quantity and quality requirements for exosomes, and consider disadvantages of distinct isolation methods.

show abstract

Novel method for extracting exosomes of hepatocellular carcinoma cells

Abstract: The new extraction method based on the nanomaterial is quick and efficient. The cancer-associated protein TGM2 can be secreted through an exosome-mediated non-classical secretion pathway, and it may be a valuable tumor marker.

Cited by 40 publications

References 28 publications

Systematic review of factors influencing extracellular vesicle yield from cell cultures

Systematic review of factors influencing extracellular vesicle yield from cell cultures

Exosome-mediated delivery of functionally active miRNA-142-3p inhibitor reduces tumorigenicity of breast cancer in vitro and in vivo

Exosome Isolation: Is There an Optimal Method with Regard to Diagnosis or Treatment?

Contact Info

Product

Resources

About