Novel method of detection of phenylpropanoids of Rhodiola roots species

Abstract

S u m m a r yThe aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography -tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.

Individual Differences in Growth and in Accumulation of Secondary Metabolites in Rhodiola rosea Cultivated in Western Siberia

“…According to Olennikov et al [54], the main class of phenolic compounds in the underground organs of R. rosea is catechins: their concentrations are 10.84 and 61.30 mg/g in the roots and rhizome, respectively. Other investigators have shown that the level of catechins in the root can vary from 4.6 mg/g (in specimens from Poland [55]) to 20 mg/g (in specimens of Indian origin [56]). Judging by our data, the total concentration of catechins is on average 1.61 times higher in the root of R. rosea than in the rhizome (mainly because of compound No.…”