Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Alazzam, Anas; Mathew, Bobby; Alhammadi, Falah

doi:10.1002/jssc.201601061

Cited by 87 publications

(52 citation statements)

References 35 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(B) Microfluidic Vortex HT chip: (1) prime; (2) infuse; (3) wash; (4) release [63]; reproduced by permission of Springer Nature. (C) DEP CTC isolation [66]; reproduced by permission of John Wiley and Sons. (D) Acoustic CTC isolation [70]; reproduced by permission of John Wiley and Sons.…”

Section: Label‐independent Technologiesmentioning

confidence: 99%

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasis

Jiang

Wang

et al. 2020

Electrophoresis

View full text Add to dashboard Cite

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasisThe prognosis of malignant tumors is challenged by insufficient means to effectively detect tumors at early stage. Liquid biopsy using circulating tumor cells (CTCs) as biomarkers demonstrates a promising solution to tackle the challenge, because CTCs play a critical role in cancer metastatic process via intravasation, circulation, extravasation, and formation of secondary tumor. However, the effectiveness of the solution is compromised by rarity, heterogeneity, and vulnerability associated with CTCs. Among a plethora of novel approaches for CTC isolation and enrichment, microfluidics leads to isolation and detection of CTCs in a cost-effective and operation-friendly way. Development of microfluidics also makes it feasible to model the cancer metastasis in vitro using a microfluidic system to mimick the in vivo microenvironment, thereby enabling analysis and monitor of tumor metastasis. This paper aims to review the latest advances for exploring the dual-roles microfluidics has played in early cancer diagnosis via CTC isolation and investigating the role of CTCs in cancer metastasis; the merits and drawbacks for dominating microfluidics-based CTC isolation methods are discussed; biomimicking cancer metastasis using microfluidics are presented with example applications on modelling of tumor microenvironment, tumor cell dissemination, tumor migration, and tumor angiogenesis. The future perspectives and challenges are discussed.

show abstract

Section: Label‐independent Technologiesmentioning

confidence: 99%

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasis

Jiang

Wang

et al. 2020

Electrophoresis

View full text Add to dashboard Cite

show abstract

Section: Single‐cell Sortingmentioning

confidence: 99%

Microfluidic Devices in the Fast‐Growing Domain of Single‐Cell Analysis

Khan

Mao

et al. 2018

Chemistry A European J

View full text Add to dashboard Cite

Recent advances in cellular analysis revealed that the seemingly identical cells are heterogeneous in term of functionality, compositions, and genetic performance. These differences cause difficulty in the diagnostic for a specific model of disease. Detection of biomolecules such as DNA, RNA, and protein or analysis of cell(s), detection of cell surface molecules, and secreted protein, can help us to improve the understanding of a targeted disease and development of new diagnostic and therapeutic approaches. A single-cell includes the minute quantity of these target molecules. Microfluidic devices have the ability to capture a single-cell and its lysate into a pico or femtoliter volumes droplet, or micro-well thus preventing dilution and limiting diffusion. In this review we described the advancement and limitations in microfluidic techniques used toward single-cells analysis.

show abstract

“…Almost all dielectric particles can be subjected to dielectrophoresis. Dielectrophoresis find application in clinical diagnostics, disease treatment, pharmacology, immunology, biotechnology, separation of cell cultures and others .…”

Section: Introductionmentioning

confidence: 99%

Barrier contactless dielectrophoresis: A new approach to particle separation

Podoynitsyn

Sorokina

Klimov

et al. 2019

Separation Science Plus

View full text Add to dashboard Cite

Barrier contactless dielectrophoresis is proposed as a method for microbiological particle separation. Design of a separation device for barrier contactless dielectrophoresis is discussed. The principle of separation device is based on formation of barriers of gradient fields in the volume of separation chamber by electrodes with passivation dielectric coating of silicon carbide. A feature of the method is absence of contact between separated samples and conductive elements of electrode structure. The proposed method is highly efficient and easy to use in comparison with dielectrophoresis on isolated structures and conventional planar systems. Computer simulation of electric field distribution over dielectrophoretic barriers is carried out, and effect of the thickness of the passivation coating on dielectrophoretic properties of barriers is shown. Forces acting on a particle (a yeast cell with a diameter of 7 μm) in separation chamber are evaluated using computer simulation data. Ability of the cell to be captured by the method of contactless barrier dielectrophoresis is theoretically predicted and confirmed experimentally, using yeast cells. The yeast cells subjected to positive dielectrophoresis can be effectively captured (up to 90%) by the separation device at the applied voltage of 20 V and 100 kHz and at flow rate of 20 mL/h.

show abstract

Novel microfluidic device for the continuous separation of cancer cells using dielectrophoresis

Cited by 87 publications

References 35 publications

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasis

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasis

Microfluidic Devices in the Fast‐Growing Domain of Single‐Cell Analysis

Barrier contactless dielectrophoresis: A new approach to particle separation

Contact Info

Product

Resources

About