Novel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human saliva

Ferreira, Francisca T.S.M.; Mesquita, Raquel B. R.; Rangel, António O. S. S.

doi:10.1016/j.talanta.2020.121183

Cited by 60 publications

(41 citation statements)

References 32 publications

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“…Both approaches are equally important for the potential applications with a big number of volunteers. The initial pH of volunteer 1 was 6.764 (4), which was the highest pH among the volunteers, indicating a healthy near-neutral environment. The other volunteers had low initial values of pH of less than 6, which can be an indication of oral and other health problems.…”

Section: Volunteersmentioning

confidence: 91%

“…The combination of the microfluidic approach and the integration of different sensors into a multifunctional labon-a-chip (LoC) system with the simple and non-invasive collection of saliva samples will lead to the realization of fast-sensing tools with good specificity and selectivity. (2) Different sensors have recently been developed for measurements of glucose (3) and nitrate and nitrite, (4) and the detection of bacterial pathogens, (5) biomarkers, (6) and diseases (7) in saliva. In addition to the detection of constituents of saliva, pH, O 2 content, and CO 2 content are important parameters that give information about the physicochemical properties of saliva and the chemical and biochemical processes that occur in the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Approach for Measurements of pH, O2, and CO2 in Saliva

Podunavac¹,

Hinić²,

Kojić³

et al. 2021

Sensors and Materials

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In this paper, we propose a microfluidic approach for measuring pH, dissolved oxygen (O 2), and carbon dioxide (CO 2) in human saliva. The proposed innovative method combines the advantages of a microfluidic approach, i.e., small amounts of samples and reagents and precise control of the experimental conditions, with rapid measurements of significant parameters of saliva. The novel design of a microfluidic chip with integrated commercially available PreSens sensors was used for examining the effect of Chlorhexidine on artificial saliva (AS), stimulated saliva (SS), and non-stimulated saliva (NSS). The measurement results showed that for persons with an initially low saliva pH, the use of Chlorhexidine increased the pH, and afterward, the pH value returned to the initial value or higher. However, measurements of volunteers with initial pH close to neutral showed that Chlorhexidine reduced the pH value, increasing the risk of erosion and demineralization. In conclusion, the proposed methodology showed potential for precise measurements of pH in saliva samples; however, further research is required to examine the influence of the sample collection method on the amounts of O 2 and CO 2 in saliva.

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Section: Volunteersmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Microfluidic Approach for Measurements of pH, O2, and CO2 in Saliva

Podunavac¹,

Hinić²,

Kojić³

et al. 2021

Sensors and Materials

View full text Add to dashboard Cite

show abstract

“…However, they anticipate that their device can be used to detect glucose in whole blood if a filtering membrane is added on top of their device's reaction zone to filter out the blood cells. Ferreira et al [34] used zinc microparticles as the reducing agent in a paper-based device to detect nitrate in human saliva.…”

Section: Biomedicalmentioning

confidence: 99%

Literature Review of the Use of Zinc and Zinc Compounds in Paper-Based Microfluidic Devices

Charbaji¹,

Heidari-Bafroui²,

Anagnostopoulos³

et al. 2021

JMMCE

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Zinc and its compounds, alloys and composites play an important role in the modern day world and find application in almost every aspect that can improve the quality of our lives. This ranges from supplements and pharmaceuticals that are meant to improve our health and wellbeing to additives meant to guard or reduce corrosion in metals. However, over the past several years, a new area of technology has been garnering a great deal of attention and has made use of zinc and its compounds. This is with reference to paper-based microfluidic technology that offers several advantages and that keeps expanding in the amount of applications it covers. In this paper, a review is offered for the applications that have used zinc or zinc compounds in paper-based microfluidic devices.

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“…Owing to their advantages, such as low cost and direct on-site measurements, microfluidic paper-based analytical devices (µPADs) and glass-based devices are also applied in the detection of nitrite [12][13][14]. Compared to the above-mentioned analytical methods, electrochemical techniques have many advantages, such as simple operation, low cost, good selectivity, fast response, excellent sensitivity, and no need for additional chemical reagents [15,16].…”

Section: Introductionmentioning

confidence: 99%

Mesoporous CoOx/C Nanocomposites Functionalized Electrochemical Sensor for Rapid and Continuous Detection of Nitrite

et al. 2021

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Nitrite is widespread in the environment, and is frequently used as an additive to extend the shelf life of meat products. However, the excess intake of nitrite can be harmful to human health. Hence, it is very important to know and control the content of nitrite in foodstuffs. In this work, by the means of self-assembly induced by solvent evaporation, we used the amphiphilic PEO-b-PS diblock copolymers resol and cobalt nitrate as a template to synthesize ordered mesoporous CoOx/C nanocomposites. Then, the CoOx/C nanocomposites were modified on a glassy carbon electrode (GCE), which showed excellent sensitivity, good selectivity, and a wide detection range for nitrite. Through cyclic voltammetry and current–time techniques, the electrochemical performance of the GCE modified with CoOx/C nanocomposites was analyzed. Under the optimized conditions, we found that anodic currents were linearly related to nitrite concentrations with a regression equation of lp (µA) = 0.36388 + 0.01616C (R2 = 0.9987) from 0.2 µM to 2500 µM, and the detection limit was 0.05 µM. Furthermore, the electrochemical sensor behaved with high reproducibility and anti-interference ability towards various organic and inorganic ions, such as NO3−, SO42−, Cl−, COOH− (Ac−), Na+, K+, Mg2+, and NH4+. Our results indicated that these CoOx/C nanocomposites could be applied in electrochemical sensors for the rapid and sensitive detection of the food preservative nitrite.

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Novel microfluidic paper-based analytical devices (μPADs) for the determination of nitrate and nitrite in human saliva

Cited by 60 publications

References 32 publications

Microfluidic Approach for Measurements of pH, O2, and CO2 in Saliva

Microfluidic Approach for Measurements of pH, O2, and CO2 in Saliva

Literature Review of the Use of Zinc and Zinc Compounds in Paper-Based Microfluidic Devices

Mesoporous CoOx/C Nanocomposites Functionalized Electrochemical Sensor for Rapid and Continuous Detection of Nitrite

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