Novel microsatellite loci in the threatened European long-snouted seahorse (Hippocampus guttulatus) for genetic diversity and parentage analysis

Pardo, Belén G.; López, Almudena; Martı́nez, Paulino; Bouza, Carmen

doi:10.1007/s10592-006-9241-7

Cited by 14 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetic diversity of microsatellites reported within other seahorse and pipefish species are generally higher: Observed diversity found within the Western Australian seahorse, Hippocampus angustus, ranged between 0.73-0.95 [18]; diversity within the Gulf pipefish, Syngnathus scovelli, ranged between 0.88-0.95, and within the dusky pipefish, Syngnathus floridae, between 0.78-0.95 [19]; diversity within the broadnosed pipefish, Syngnathus typhle between 0.55-0.95 [19]; and diversity within the pot bellied seahorse, Hippocampus abdominalis, between 0.87 and 0.98 [20]. Only the observed diversities within the long snouted seahorse, Hippocampus guttulatus, between 0.31 and 0.85, was found to be similar to those reported here [21], and diversity within the spotted seahorse, Hippocampus kuda, 0.00-0.30 was found to be lower [22]. Thus, the range of diversity within leafy seadragons reported here is low but falls within the range reported for some other seahorse species.…”

Section: Syngnathus Leptorhynchus)mentioning

confidence: 89%

Novel Microsatellite Loci Variation and Population Genetics within Leafy Seadragons, Phycodurus eques

et al. 2014

View full text Add to dashboard Cite

Novel leafy seadragon (Phycodurus eques) microsatellite loci were developed via standard cloning techniques and tested for use in population genetics studies. Six out of a total of twelve microsatellites tested were usable for population analysis. Seadragon samples from Western Australia (N = 6), Southern Australia (N = 11), and a captive group (N = 11) were analyzed. Here, we present leafy seadragon microsatellite primer sequences for all 12 loci and population genetics statistics for the six loci that amplified consistently and displayed adequate variability to estimate population parameters, such as diversity, population differences, and relatedness. Observed heterozygosities ranged from 0.225 to 0.926 and expected heterozygosities ranged from 0.278 to 0.650. Pairwise differences among populations (F ST estimates) from samples collected off the southern coast of Western and South Australia, and captive animals ranged from a low of 0.188 between Southern Australia and captive animals, to a high of 0.212 between Western Australia and captive animals. Statistical assignment analyses suggested between one and three populations. Percent first order relatives among individuals was high and ranged from 40 within Western Australia to 55 within captive animals. These loci were tested on other species including weedy seadragons (Phyllopteryx taeniolatus), as well as assorted

show abstract

Section: Syngnathus Leptorhynchus)mentioning

confidence: 89%

Novel Microsatellite Loci Variation and Population Genetics within Leafy Seadragons, Phycodurus eques

et al. 2014

View full text Add to dashboard Cite

show abstract

“…DNA samples of 11 adult seahorses from NW Spain (42834 0 N, 9800 0 W), identified as H. guttulatus based on morphological and reproductive traits (Planas et al 2008), were included for phylogenetic analysis. Genomic DNA was isolated using Chelex Ò resin (Pardo et al 2007) in most samples. For very small pieces of skin filaments, DNA was purified with NucleoSpin Ò Tissue XS kit (Macherey-Nagel) and when necessary amplified using GenomiPhi V2 kit (GE Healthcare).…”

Section: Methodsmentioning

confidence: 99%

“…Twelve microsatellites from H. guttulatus (Pardo et al 2007), the four-ones from H. abdominalis (Wilson and Martin-Smith 2007) and four selected loci from H. capensis, under polymorphism and Hardy-Weinberg (HW) equilibrium criteria (Hcal25, 27, 33 and 36; Galbusera et al 2007), were tested. Ten loci were finally used (Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Ten loci were finally used (Table 1). Amplifications were carried out as described by Pardo et al (2007), at 60°C for annealing (except Hgu-USC13, 58°C). Genotyping was performed on an ABI 3730xl DNA Analyzer using GENEMAPPER v3.7 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…Different genetic markers have shown strong molecular support for seahorse species identification (Casey et al 2004;Teske et al 2004;Sanders et al 2008). Also, microsatellites have proven to be helpful to analyze genetic diversity and mating system of seahorses (Kvarnemo et al 2000;Galbusera et al 2007;Pardo et al 2007;Wilson and Martin-Smith 2007), and to evaluate interspecific hybridization in syngnathids (Wilson 2006).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Species identification and genetic structure of threatened seahorses in Gran Canaria Island (Spain) using mitochondrial and microsatellite markers

et al. 2010

Self Cite

View full text Add to dashboard Cite

A non-invasive DNA analysis of seahorse populations was carried out after extensive underwater surveys in Gran Canaria Island (Spain). In this geographical area, the presence of two species, Hippocampus hippocampus and H. guttulatus, has been previously reported. Sequencing of 16S ribosomal DNA (16S rDNA) was used for specific identification of live seahorses sampled in situ, as a previous step to evaluate genetic structure based on ten microsatellite markers. Phylogenetic analyses revealed the presence of a single species, H. hippocampus, in the seahorse communities found at Gran Canaria. No evidences of H. guttulatus or interspecific hybrids were found based on 16S rDNA and microsatellite data. The nuclear markers revealed low genetic diversity and lack of population structure across populations of Gran Canaria Island, with evidence of small population sizes. This study provides critical information to support conservation strategies of Gran Canaria seahorses.

show abstract

Non-lethal dorsal fin sampling for stable isotope analysis in seahorses

Valladares

Planas

2012

Aquat Ecol

View full text Add to dashboard Cite

Sampling collection for stable isotope analysis has traditionally involved the sacrifice of the animal. Seahorses (Hippocampus spp.) are listed as threatened by the Convention on International Trade in Endangered Species (http://www.cites.org) and consequently lethal sampling is undesirable. We evaluated the adequacy of dorsal fin tissue of adult seahorses Hippocampus guttulatus for stable isotope analysis as an alternative to lethal tissue sampling. Three seahorse tissues (dorsal fin, muscle and liver) were analysed for comparisons of δ 15 N and δ 13 C values. Similarities found between δ 15 N and δ 13 C values in dorsal fin and muscle tissue of H. guttulatus suggests that both tissues are adequate for stable isotope analysis to understand feeding ecology of seahorses. However, considering the threatened status of the species, dorsal fin tissue would be recommended in adult seahorses as a non-lethal sampling. The effect of lipid extraction on carbon and nitrogen stable isotope values was also evaluated in each seahorse tissues. Significant effects of lipids extraction did only occur for δ 13 C values in muscle and liver. It was found that lipid removal was not necessary to perform SIA in dorsal fin tissues. Due to the limited availability of fin tissue obtained from fin-clipping in seahorses, the relationship between the mass/surface of dorsal fin clip and stable isotope values was analysed. δ 15 N and δ 13 C values in fin samples were found to be independent of the size of fin analysed. According to our study, the use of fin-clipping sampling, with a minimum surface analysed of 12.74 mm 2 , was found to be an adequate method for SIA in seahorses.

show abstract

Novel microsatellite loci in the threatened European long-snouted seahorse (Hippocampus guttulatus) for genetic diversity and parentage analysis

Cited by 14 publications

References 9 publications

Novel Microsatellite Loci Variation and Population Genetics within Leafy Seadragons, Phycodurus eques

Novel Microsatellite Loci Variation and Population Genetics within Leafy Seadragons, Phycodurus eques

Species identification and genetic structure of threatened seahorses in Gran Canaria Island (Spain) using mitochondrial and microsatellite markers

Non-lethal dorsal fin sampling for stable isotope analysis in seahorses

Contact Info

Product

Resources

About