Novel model for study of human sperm chemotaxis

Villanueva-Díaz, Carlos; Arias-Martínez, Joel; Bustos-López, Hugo; Vadillo-Ortega, Felipe

doi:10.1016/s0015-0282(16)55209-1

Cited by 28 publications

(16 citation statements)

References 12 publications

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“…FF causes sperm accumulation [4,30,31] by way of chemotaxis [3,7,16]. On the basis of the observation that the chemotactic activity of FF is retained in the supernatant after precipitation with 90% acetone but is mainly in the pellet after precipitation with 100% acetone, Ralt et al [7] concluded that the chemoattractant in FF is nonhydrophobic, possibly a short peptide or a similar molecule.…”

Section: Chemotactic Activity Of Ff Is Not Due To Progesteronementioning

confidence: 99%

Human Sperm Chemotaxis: Is Progesterone a Chemoattractant?1

Jaiswal¹,

Tur-Kaspa²,

Dor³

et al. 1999

Biology of Reproduction

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Follicular fluid (FF) induces sperm chemotaxis in human spermatozoa. Progesterone also causes sperm accumulation. However, sperm accumulation can be caused by chemotaxis, chemokinesis, and trapping of various kinds. It has been suggested that progesterone also induces chemotaxis in human spermatozoa. In view of the physiological significance of sperm chemotaxis in human fertilization and its potential clinical implications, it is important to determine unequivocally whether chemotaxis is induced by progesterone and, if so, whether progesterone in FF is the chemoattractant. To resolve these questions we looked for characteristic changes in the direction of sperm swimming toward pure progesterone as well as toward FF before and after progesterone removal. Progesterone caused sperm accumulation and hyperactivation-like motility, but it caused very few changes in the direction of sperm swimming that are characteristic of chemotaxis. Removal of progesterone (and other steroids) from FF by charcoal treatment abolished the sperm hyperactivation-like motility but not sperm chemotaxis. These results suggest that while progesterone might be a weak chemoattractant, it is not the major chemoattractant in FF. Progesterone probably causes human sperm accumulation mainly by inducing hyperactivation-like motility and, as a consequence, sperm trapping.

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Section: Chemotactic Activity Of Ff Is Not Due To Progesteronementioning

confidence: 99%

Human Sperm Chemotaxis: Is Progesterone a Chemoattractant?1

Jaiswal¹,

Tur-Kaspa²,

Dor³

et al. 1999

Biology of Reproduction

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“…False-positive results may be obtained because non-diluted, or diluted less than 10-fold, follicular fluids (or even human or bovine serum) may cause nonspecific sperm accumulation by virtue of trapping [4]. In view of this information, earlier conclusions about the occurrence of sperm chemotaxis to follicular fluid [20][21][22] and about the inability to observe chemotaxis [23] should be evaluated with great caution. All these published studies used nondiluted or only slightly diluted (1:4) follicular fluids.…”

Section: Physiological Parameters That Affect Detectability Of Chemotmentioning

confidence: 99%

“…Attraction of human spermatozoa to follicular fluid, correlated with egg fertilization, was indeed demonstrated, but the issue of whether or not the attraction was due to chemotaxis was not determined [19]. Other studies that claimed observation of sperm chemotaxis to follicular fluid (e.g., [20][21][22]), did not distinguish between true chemotaxis and other processes that might cause sperm accumulation. In addition to reports that unjustifiably claimed to have demonstrated chemotaxis, a recent study reported a random distribution of spermatozoa in a gradient of follicular fluid and was unable to demonstrate chemotaxis [23].…”

Section: Introductionmentioning

confidence: 99%

Chemotaxis and Chemokinesis of Human Spermatozoa to Follicular Factors1

Ralt

Manor

Cohen-Dayag

et al. 1994

Biology of Reproduction

134

122

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Human spermatozoa accumulate in vitro in diluted follicular fluids obtained from follicles from which the eggs have been fertilized. Using capillary assays under a variety of experimental conditions (ascending or descending gradients of follicular fluid, or no gradient at all) and microscopic assays in which individual spermatozoa could be followed, we found that the sperm accumulation in follicular fluid was the result of both sperm chemotaxis and chemokinesis and eventually hyperactivation-like motility. We determined the optimal conditions for sperm accumulation, which involved sperm preincubation (possibly to induce sperm capacitation) and proper dilution of follicular fluid. In all the assays, the net accumulation was low, probably reflecting the chemotactic responsiveness of only a small fraction of the sperm population at any given time. We partially fractionated follicular fluid in a Centricon microconcentrator (Amicon, Danvers, MA) and by acetone precipitation, and found that at least one of the chemotactic factors is a small (< 10-kDa) molecule that is probably nonhydrophobic. This is the first time that sperm chemotaxis and chemokinesis in response to a follicular factor(s) in mammals has been established and has been distinguished from other processes that might cause sperm accumulation. The physiological significance of these findings is discussed.

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“…a) la fraction des spermatozoides chimiotactiques dans une population donn6e de spermatozo~des est faible (2-12% chez l'humain [18], et environ 10% chez la souris [17,19]) ; b) la sensibilit6 des spermatozo~des au chimiotactisme est temporaire et n'est acquise qu'une seule fois dans la vie d'un spermatozoide [18] ; [42] Ralt et al [32] Makler et al [12,13] Villanueva-Diaz et al [38] Ralt et al [11] Cohen-Dayag et al [18] Navarro et al [47] Giojalas and Rovasio [19] Oliveira et al [17] Jaiswal et al [16] c) il y a un remplacement continu des spermatozoides chimiotactiques au sein d'une population de spermatozo~des -les spermatozoides chimiotactiques perdent leur activit6 alors que d'autres l'acqui6rent [18]. Ces observations soulev6rent la possibilit6 que les spermatozo~des ne soient s61ectivement chimiotactiques qu'~ une certaine 6tape physiologique, probablement au stade de la capacitation [20].…”

Section: Caracti~ristiques Du Chimio-tactisme Des Spermatozoidesunclassified

Le chimiotactisme des spermatozoïdes a-t-il un rôle dans la fécondation?

Eisenbach

2000

Androl.

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Le chimiotactisme des spermatozoides par le fluide folliculaire a 6t6 6tabli par diff6rents moyens en ce qui concerne les spermatozoides de l'homme et de la souris. Seule une petite fraction d'une population donn6e de spermatozoides (en moyenne environ 10%) pr~sente une sensibilit6 chimiotactique, et cette fraction constitue les spermatozoides capacit~s. La sensibilit6 chimiotactique et l'6tat capacit~ sont tous les deux transitoires (avec une dur~e de vie de 50 minutes ~ 4 heures) et ne surviennent qu'une seule fois dans la vie d'un spermatozoide. On a propos6 comme r61e au chimiotactisme des spermatozoides chez les mammif~res (du moins chez l'homme) le recrutement s61ectif de spermatozoides capacit6s pour la f6condation de l'ovocyte ; le r61e du remplacement continu des spermatozoides sensibles au chimiotactisme / capacit6s serait de prolonger la dur6e de temps pendant laquelle des spermatozoides capacit6s seraient disponibles dans le tractus g~nital f6minin. Les substances chimio-attractives des spermatozoides n'ont pas 6t6 identifi6es mais elles semblent ~tre des peptides thermo-stables. La localisation in vivo du chimiotactisme des spermatozoides est inconnue ; de nombreuses localisations potentielles sont discut~es dans cet article.

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Novel model for study of human sperm chemotaxis

Cited by 28 publications

References 12 publications

Human Sperm Chemotaxis: Is Progesterone a Chemoattractant?1

Human Sperm Chemotaxis: Is Progesterone a Chemoattractant?1

Chemotaxis and Chemokinesis of Human Spermatozoa to Follicular Factors1

Le chimiotactisme des spermatozoïdes a-t-il un rôle dans la fécondation?

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