Novel molecularly imprinted monolithic column for selective on‐line extraction of ciprofloxacin from human urine

Abstract

Water-compatible ciprofloxacin-imprinted monolithic columns were synthesized in water-containing systems for selective extraction of ciprofloxacin from human urine samples. Methanol-water (10:3, v/v) was used as a porogenic solvent and the obtained monolithic imprinted polymers reveal high selectivity to ciprofloxacin in an aqueous environment; the affinity can be easily controlled by adjusting the pH of the mobile phase. Owing to the unique porous structure and flow-through channels existing in the network skeleton of the monolithic MIP, urine samples could be directly injected into the column, proteins and other biological matrix were quickly washed out and ciprofloxacin was selectively retained and enriched. Good linearity was obtained from 0.08 to 400 mg/L (r=0.998) with the relative standard deviations less than 3.6%. The limit of detection of the method was 0.04 mg/L and the recoveries were more than 94.5% at three different concentrations. Moreover, by increasing the injection volume to 2.0 mL, the sensitivity of the method could be improved 100-fold according to the peak height of ciprofloxacin. This expedient greatly simplified the overall procedure, resulting in a rapid and efficient sample analysis while maintaining precision and accuracy.

Determination of quinolones in milk samples using a combination of magnetic solid‐phase extraction and capillary electrophoresis

“…Tricyclic QNs (Oxa. Ac, FLU) are preconcentrated using phenyl adsorbents by π–π interactions []. A mixed‐polarity adsorbent is required to ensure homogeneity during the retention and elution of QNs.…”

Monolithic media for applications in affinity chromatography

“…The presence of both high‐ and low‐affinity binding sites for the template molecule ciprofloxacin was determined by Scatchard analysis (Fig. 9) 159. Structurally related compounds eluted earlier than the template molecules proving the high specificity of the molecularly imprinted monoliths.…”

“…Preparation of MIPs was reported using ciprofloxacin 159, norfloxacin 160, metribuzin 161, and protocatechuic acid (3,4‐dihydroxybenzoic acid) 162 as templates. The presence of both high‐ and low‐affinity binding sites for the template molecule ciprofloxacin was determined by Scatchard analysis (Fig.…”

Molecularly imprinted polymers as affinity‐based separation media for sample preparation

“…They are desorbed by gradient elution from 35% ACN in water to 50% ACN [72]. An MIP for ciprofloxacin prepared in a mixture methanol and water was applied to selective on-column extraction of ciprofloxacin in human urine [73,74]. Urine samples were directly injected onto the MIP column, ordinary components of urine were eluted and ciprofloxacin was enriched.…”