Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C C double bond migration useful for conjugated fatty acid synthesis

Kishino, Shigenobu; Park, Si‐Bum; Takeuchi, Mitsuaki; Yokozeki, Kenzo; Shimizu, Sakayu; Ogawa, Jun

doi:10.1016/j.bbrc.2011.11.022

Cited by 57 publications

(55 citation statements)

References 24 publications

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“…In addition, our recent research suggested the possibility that lipid metabolism by gut microbiota affects the health of the host by modifying FA composition ( 23 ). Therefore, our evidence-based studies on lipid metabolism by gut bacteria, including C. bifermentans (in this study) and Lactobacillus (21)(22)(23)34 ), should serve to maintain and improve the health of the host.…”

Section: Discussionmentioning

confidence: 83%

Biohydrogenation of C20 polyunsaturated fatty acids by anaerobic bacteria

Sakurama

Kishino

Mihara

et al. 2014

Journal of Lipid Research

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Section: Discussionmentioning

confidence: 83%

Biohydrogenation of C20 polyunsaturated fatty acids by anaerobic bacteria

Sakurama

Kishino

Mihara

et al. 2014

Journal of Lipid Research

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“…Our subsequent metabolic analysis indicated that 10-hydroxy-12-octadecenoic acid is an intermediate of CLA synthesis, and further investigations of hydroxy fatty acid metabolism by lactic acid bacteria revealed that CLA is produced from hydroxy fatty acids such as ricinoleic acid in castor oil (22)(23)(24)(25). In cell-free extracts from this strain, we identified the enzymes involved in CLA synthesis (26). Three enzymes, CLA-HY, CLA-DH, and CLA-DC, are necessary for synthesis of conjugated fatty acids such as CLA.…”

mentioning

confidence: 84%

Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition

Kishino

Takeuchi²,

Park

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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In the representative gut bacterium Lactobacillus plantarum, we identified genes encoding the enzymes involved in a saturation metabolism of polyunsaturated fatty acids and revealed in detail the metabolic pathway that generates hydroxy fatty acids, oxo fatty acids, conjugated fatty acids, and partially saturated transfatty acids as intermediates. Furthermore, we observed these intermediates, especially hydroxy fatty acids, in host organs. Levels of hydroxy fatty acids were much higher in specific pathogen-free mice than in germ-free mice, indicating that these fatty acids are generated through polyunsaturated fatty acids metabolism of gastrointestinal microorganisms. These findings suggested that lipid metabolism by gastrointestinal microbes affects the health of the host by modifying fatty acid composition.biohydrogenation | hydratase | fatty acid isomerase | conjugated linoleic acid | lipid nutrition

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“…L. plantarum AKU 1009a can transform not only LA but also a-linolenic acid (cis-9,cis-12,cis-15-octadecatrienoic acid) and g-linolenic acid (cis-6,cis-9,cis-12-octadecatrienoic acid) into the corresponding conjugated fatty acids (19e24). We also showed that CLA is synthesized, in part, through the reactions of a newly discovered polyunsaturated fatty acid saturation metabolism in L. plantarum AKU1009 a (25,26). The novel saturation metabolism consisted of four enzymes: CLA-HY (hydratase/dehydratase), CLA-DH (dehydrogenase), CLA-DC (isomerase), and CLA-ER (enonereductase) (25,26).…”

mentioning

confidence: 94%

“…We also showed that CLA is synthesized, in part, through the reactions of a newly discovered polyunsaturated fatty acid saturation metabolism in L. plantarum AKU1009 a (25,26). The novel saturation metabolism consisted of four enzymes: CLA-HY (hydratase/dehydratase), CLA-DH (dehydrogenase), CLA-DC (isomerase), and CLA-ER (enonereductase) (25,26). CLA-HY, CLA-DH, and CLA-DC are responsible for CLA synthesis from LA (25) and CLA-ER is a key enzyme for the saturation metabolism (26).…”

mentioning

confidence: 95%

Characterization of the linoleic acid Δ9 hydratase catalyzing the first step of polyunsaturated fatty acid saturation metabolism in Lactobacillus plantarum AKU 1009a

Takeuchi

Kishino

Hirata

et al. 2015

Journal of Bioscience and Bioengineering

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Linoleic acid Δ9 hydratase, which is involved in linoleic acid saturation metabolism of Lactobacillus plantarum AKU 1009a, was cloned, expressed as a his-tagged recombinant enzyme, purified with an affinity column, and characterized. The enzyme required FAD as a cofactor and its activity was enhanced by NADH. The maximal activities for the hydration of linoleic acid and for the dehydration of 10-hydroxy-cis-12-octadecenoic acid (HYA) were observed at 37 °C in buffer at pH 5.5 containing 0.5 M NaCl. Free C16 and C18 fatty acids with cis-9 double bonds and 10-hydroxy fatty acids served as substrates for the hydration and dehydration reactions, respectively. The apparent Km value for linoleic acid was estimated to be 92 μM, with a kcat of 2.6∙10(-2) s(-1) and a Hill factor of 3.3. The apparent Km value for HYA was estimated to be 98 μM, with a kcat of 1.2∙10(-3) s(-1).

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Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C C double bond migration useful for conjugated fatty acid synthesis

Cited by 57 publications

References 24 publications

Biohydrogenation of C20 polyunsaturated fatty acids by anaerobic bacteria

Biohydrogenation of C20 polyunsaturated fatty acids by anaerobic bacteria

Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition

Characterization of the linoleic acid Δ9 hydratase catalyzing the first step of polyunsaturated fatty acid saturation metabolism in Lactobacillus plantarum AKU 1009a

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