Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots

et al. 2023

Preprint

Self Cite

Background: Osteochondral allograft (OCA) transplantation involves grafting of natural hyaline cartilage and supporting subchondral bone into the cartilage defect area of a joint to recover its biomechanical and tissue structure. However, differing biomechanical properties and donor-host matching may impair the integration of articular cartilage (AC). This study analyzed the biomechanical properties of the AC in different regions of different sites of the knee joint and provides a novel approach to OCA transplantation. Methods： Intact stifle joints from skeletally mature pigs were collected from a local abattoir less than 8 h after slaughter. OCAs were collected from different regions of the joints. The patella and thetibial plateau were divided into medial and lateral regions, while the trochlea and femoral condyle were divided into six regions. The OCAs were analyzed and compared for Young’s modulus, the compressive modulus, and cartilage thickness. Results:Young’s modulus, cartilage thickness and compressive modulus of OCA were significantly different in different regions of the joints. A negative correlation was observed between Young's modulus and the proportion of the subchondral bone (r=−0.4241, P<0.0001). Cartilage thickness was positively correlated with Young’s modulus (r=0.4473, P<0.0001) and the compressive modulus (r=0.3678, P<0.0001). Conclusions: During OCA transplantation, OCAs may be xenotransplanted into similar regions, or at the closest possible regions to ensure consistency of the biomechanical properties and cartilage thickness of the donor and recipient and, thus, easy integration with the surrounding tissue. A 7-mm depth achieved a higher Young's modulus, and may represent the ideal length.

“…The load-indentation determined Young's modulus using the Hertz model. [7] Data were collected after the probe made contact with the cartilage.…”

Section: Nanoindentation Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biomechanical Properties of Articular Cartilage in Different Regions and Sites of the Knee Joint: Acquisition of Osteochondral Allografts

Lin

et al. 2023

Preprint

Self Cite

“…Compared with traditional metal-containing QDs, CDs have the features of simple synthetic material stability, low cytotoxicity, and biological compatibility. 17,18 These characteristics make CDs have a wide range of application prospects in biological imaging, 19 information encryption, 20 supercapacitors, 21 optoelectronic devices, 22 ion detection, 23 biomedicine 24,25 etc. While most of the research in this area is based on blue emission CDs, 26,27 FLFIA has blue background interference; therefore, the synthesis of CDs of other colors becomes very meaningful.…”

Section: Introductionmentioning

confidence: 99%

Duplex-immunoassay of ovarian cancer biomarker CA125 and HE4 based carbon dot decorated dendritic mesoporous silica nanoparticles

Ding

2023

Analyst

“…Articular cartilage is located throughout the body and performs various functions. However, it is often damaged as a result of trauma, aging, and disease (Guo et al, 2022). Articular cartilage injury frequently leads to progressive tissue degradation, joint pain, dysfunction, and degenerative arthritis (Borrelli et al, 2019;Stefani et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Research progress of self-assembling peptide hydrogels in repairing cartilage defects

Yang

et al. 2022

Front. Mater.

Due to the lack of blood vessels, nerves and lymphatic vessels, the capacity of articular cartilage to heal is extremely limited. Once damaged, it is urgent for articular cartilage to repair the injury. In recent years, there has been an increase in cartilage tissue engineering studies. Self-assembling peptide hydrogel as a kind of hydrogels composed of peptides and water is widely used in cartilage tissue engineering. Under noncovalent interactions such as electrostatic interaction, hydrophobic interaction, hydrogen bonding and pi-pi stacking force, peptides self-assemble into three-dimensional (3D) structures that mimic the natural extracellular matrix and allow cells to grow, proliferate and differentiate. Because SAPHs have excellent biocompatibility and biodegradability, variable mechanical properties, low immunogenicity, injectability, and the ability to load cells and bioactive substances, many researchers utilized them to promote the repair and regeneration of articular cartilage after damage. Therefore, the purpose of this review is to sum up the composition, injury characteristics, and treatments of articular cartilage, as well as the action of SAPHs in repairing articular cartilage damage.