Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro

Hiippala, Kaisa; Barreto, Gonçalo; Burrello, Claudia; Díaz‐Basabe, Angélica; Suutarinen, Maiju; Kainulainen, Veera; Bowers, Jolene R.; Lemmer, Darrin; Engelthaler, David M.; Eklund, Kari K.; Facciotti, Federica; Satokari, Reetta

doi:10.3389/fmicb.2020.575455

Cited by 151 publications

(92 citation statements)

References 71 publications

(99 reference statements)

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“…Clostridia is the primary producer of SCFAs, which are a significant energy source in colonocytes and are critical to maintain gut health (38). Odoribacter (39), Prevotella (40), Oscillospira (41), Paraprevotella and Ruminococcus (42), the abundance of which is elevated by stigmasterol, also synthetize SCFAs, particularly butyrate. Simultaneously, stigmasterol increased the expression levels of SCFAs, comprising acetate, propionate, and butyrate, in faeces.…”

Section: Discussionmentioning

confidence: 99%

Stigmasterol Restores the Balance of Treg/Th17 Cells by Activating the Butyrate-PPARγ Axis in Colitis

Wen

Zhong

et al. 2021

Front. Immunol.

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Inflammatory bowel disease (IBD) is a chronic inflammatory disorder with gut microbiota disequilibrium and regulatory T (Treg)/T helper 17 (Th17) immune imbalance. Stigmasterol, a plant-derived sterol, has shown anti-inflammatory effects. Our study aimed to identify the effects of stigmasterol on experimental colitis and the related mechanisms. Stigmasterol treatment restored the Treg/Th17 balance and altered the gut microbiota in a dextran sodium sulfate (DSS)-induced colitis model. Transplantation of the faecal microbiota of stigmasterol-treated mice significantly alleviated inflammation. Additionally, stigmasterol treatment enhanced the production of gut microbiota-derived short-chain fatty acids (SCFAs), particularly butyrate. Next, human naïve CD4+ T cells sorted from IBD patients were cultured under Treg- or Th17-polarizing conditions; butyrate supplementation increased the differentiation of Tregs and decreased Th17 cell differentiation. Mechanistically, butyrate activated peroxisome proliferator-activated receptor gamma (PPARγ) and reprogrammed energy metabolism, thereby promoting Treg differentiation and inhibiting Th17 differentiation. Our results demonstrate that butyrate-mediated PPARγ activation restores the balance of Treg/Th17 cells, and this may be a possible mechanism, by which stigmasterol attenuates IBD.

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Section: Discussionmentioning

confidence: 99%

Stigmasterol Restores the Balance of Treg/Th17 Cells by Activating the Butyrate-PPARγ Axis in Colitis

Wen

Zhong

et al. 2021

Front. Immunol.

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“…One compelling study hypothesizes that fecal fat selects for a proinflammatory microbiota, in particular increased abundance of E. coli ( 35 ). Furthermore, Odoribacter and Bacteroides , both of which are decreased in pwCF, have both been shown to reduce production of the proinflammatory cytokine IL-8 by gut epithelial cells ( 33 , 109 ). Additionally, Odoribacter promoted secretion of the anti-inflammatory cytokine IL-10 by peripheral blood mononuclear cells (PBMCs) ( 109 ).…”

Section: Mechanisms Of Microbial Influencementioning

confidence: 99%

The Gut-Lung Axis in Cystic Fibrosis

Price

O’Toole

2021

J Bacteriol

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Cystic Fibrosis (CF) is a heritable, multi-organ disease that impacts all tissues that normally express CFTR protein. While importance of the airway microbiota has long been recognized, the intestinal microbiota has only recently been recognized as an important player in both intestinal and lung health outcomes for persons with CF (pwCF). Here, we summarize current literature related to the gut-lung axis in CF, with a particular focus on three key ideas: Mechanisms through which microbes influence the gut-lung axis, drivers of microbiota alterations, and the potential for intestinal microbiota remediation.

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“…Remarkably, the authors observed that A. muciniphila EVs enhanced tight junction gene expression and down-regulated toll-like receptor (TLR) gene expression, which suggested a potential role for A. muciniphila EVs in intestinal barrier permeability and fortification as well as in inflammation reduction [ 9 ]. The protective role against inflammatory responses mediated by commensal and/or probiotic bacterial EVs was ratified by the studies of Hiippala et al [ 59 ], which showed that commensal Odoribacter splanchnicus 57 EVs possessed immunomodulatory properties, mitigating the production of the pro-inflammatory cytokine IL-8 in enterocyte cultures treated with E. coli -lipopolysaccharide (LPS). The anti-inflammatory properties of O. splanchnicus could potentially rely on sphingolipids, such as sulfobacin B [ 59 ].…”

Section: Role Of Gut Microbiota and Probiotic-derived Extracellular Vesicles On Inflammation Obesity And Diabetesmentioning

confidence: 99%

“…The protective role against inflammatory responses mediated by commensal and/or probiotic bacterial EVs was ratified by the studies of Hiippala et al [ 59 ], which showed that commensal Odoribacter splanchnicus 57 EVs possessed immunomodulatory properties, mitigating the production of the pro-inflammatory cytokine IL-8 in enterocyte cultures treated with E. coli -lipopolysaccharide (LPS). The anti-inflammatory properties of O. splanchnicus could potentially rely on sphingolipids, such as sulfobacin B [ 59 ]. In addition, Kang et al [ 117 ] also showed that A. muciniphila EVs pre-treatment of a colon epithelial cell line could hinder the upregulation of pro-inflammatory cytokines induced by pathogenic E. coli EVs.…”

Section: Role Of Gut Microbiota and Probiotic-derived Extracellular Vesicles On Inflammation Obesity And Diabetesmentioning

confidence: 99%

“…The characterization of gut microbiota and probiotic-derived EV cargo has been mainly focused on the analysis of the proteome and occasionally also the lipidome and the genomic DNA [ 4 , 14 , 16 , 23 , 25 , 45 , 59 , 75 , 98 , 113 ]. Of note, as previously described, the effector molecules responsible for the effect of probiotic- and gut microbiota-derived EVs have been identified [ 25 , 45 , 113 ].…”

Section: Role Of Gut Microbiota and Probiotic-derived Extracellular Vesicles On Inflammation Obesity And Diabetesmentioning

confidence: 99%

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Effects of gut microbiota–derived extracellular vesicles on obesity and diabetes and their potential modulation through diet

et al. 2021

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Obesity and diabetes incidence rates are increasing dramatically, reaching pandemic proportions. Therefore, there is an urgent need to unravel the mechanisms underlying their pathophysiology. Of particular interest is the close interconnection between gut microbiota dysbiosis and obesity and diabetes progression. Hence, microbiota manipulation through diet has been postulated as a promising therapeutic target. In this regard, secretion of gut microbiota–derived extracellular vesicles is gaining special attention, standing out as key factors that could mediate gut microbiota-host communication. Extracellular vesicles (EVs) derived from gut microbiota and probiotic bacteria allow to encapsulate a wide range of bioactive molecules (such as/or including proteins and nucleic acids) that could travel short and long distances to modulate important biological functions with the overall impact on the host health. EV-derived from specific bacteria induce differential physiological responses. For example, a high-fat diet–induced increase of the proteobacterium Pseudomonas panacis–derived EV is closely associated with the progression of metabolic dysfunction in mice. In contrast, Akkermansia muciniphila EV are linked with the alleviation of high-fat diet–induced obesity and diabetes in mice. Here, we review the newest pieces of evidence concerning the potential role of gut microbiota and probiotic-derived EV on obesity and diabetes onset, progression, and management, through the modulation of inflammation, metabolism, and gut permeability. In addition, we discuss the role of certain dietary patterns on gut microbiota–derived EV profile and the clinical implication that dietary habits could have on metabolic diseases progression through the shaping of gut microbiota–derived EV.

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Novel Odoribacter splanchnicus Strain and Its Outer Membrane Vesicles Exert Immunoregulatory Effects in vitro

Cited by 151 publications

References 71 publications

Stigmasterol Restores the Balance of Treg/Th17 Cells by Activating the Butyrate-PPARγ Axis in Colitis

Stigmasterol Restores the Balance of Treg/Th17 Cells by Activating the Butyrate-PPARγ Axis in Colitis

The Gut-Lung Axis in Cystic Fibrosis

Effects of gut microbiota–derived extracellular vesicles on obesity and diabetes and their potential modulation through diet

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