Novel Optical Techniques for Imaging Microcirculation in the Diabetic Foot

Mennes, Onno A.; Netten, Jaap J. van; Slart, Riemer H. J. A.; Steenbergen, Wiendelt

doi:10.2174/1381612824666180302141902

Cited by 31 publications

(25 citation statements)

References 86 publications

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“…Next to this, Yang et al 58 found that the degree of PWS blanching correlates well to the decrease in perfusion measured by LSCI. Finally, as pointed out by Mennes et al, 59 LSCI could be of help in the assessment of diabetic foot ulcers that are related to local ischemia. They point out that no such study has yet been performed on the diabetic foot population even though there seems to be a lot of potential based on a comparable study by others.…”

Section: Dermatologymentioning

confidence: 86%

Clinical applications of laser speckle contrast imaging: a review

et al. 2019

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When a biological tissue is illuminated with coherent light, an interference pattern will be formed at the detector, the so-called speckle pattern. Laser speckle contrast imaging (LSCI) is a technique based on the dynamic change in this backscattered light as a result of interaction with red blood cells. It can be used to visualize perfusion in various tissues and, even though this technique has been extensively described in the literature, the actual clinical implementation lags behind. We provide an overview of LSCI as a tool to image tissue perfusion. We present a brief introduction to the theory, review clinical studies from various medical fields, and discuss current limitations impeding clinical acceptance.

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Section: Dermatologymentioning

confidence: 86%

Clinical applications of laser speckle contrast imaging: a review

et al. 2019

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“…However, clinicians are not able to assess the structural or physiological information of DFU by visual inspection. The assessment for ischemia in such cases can also be assessed by ankle brachial indexes and clinical examination along with Doppler ultrasound and toe pressures measurements . In all these cases, the key marker of healing status‐perfusion is not assessed in an objective way.…”

Section: Discussionmentioning

confidence: 99%

In vivo label‐free functional photoacoustic monitoring of ischemic reperfusion

Dinish

Goh

et al. 2019

Journal of Biophotonics

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Pressure ulcer formation is a common problem among patients confined to bed or restricted to wheelchairs. The ulcer forms when the affected skin and underlying tissues go through repeated cycles of ischemia and reperfusion, leading to inflammation. This theory is evident by intravital imaging studies performed in immune cell–specific, fluorescent reporter mouse skin with induced ischemia‐reperfusion (I‐R) injuries. However, traditional confocal or multiphoton microscopy cannot accurately monitor the progression of vascular reperfusion by contrast agents, which leaks into the interstitium under inflammatory conditions. Here, we develop a dual‐wavelength micro electro mechanical system (MEMS) scanning–based optical resolution photoacoustic microscopy (OR‐PAM) system for continuous label‐free functional imaging of vascular reperfusion in an IR mouse model. This MEMS‐OR‐PAM system provides fast scanning speed for concurrent dual‐wavelength imaging, which enables continuous monitoring of the reperfusion process. During reperfusion, the revascularization of blood vessels and the oxygen saturation (sO2) changes in both arteries and veins are recorded, from which the local oxygen extraction ratios of the ischemic tissue and the unaffected tissue can be quantified. Our MEMS‐OR‐PAM system provides novel perspectives to understand the I‐R injuries. It solves the problem of dynamic label‐free functional monitoring of the vascular reperfusion at high spatial resolution.

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“…In dentistry and oral science LSCI has been applied to image the gingival blood flow, dental pulp flow. 41,42 In cardio vascular studies there are numerous uses of LSCI. A study was published regarding the evaluation of the forearm endothelium presenting notably impaired vasodilatory response with type I diabetic patient.…”

Section: Other Application Of Lscimentioning

confidence: 99%

A review of the clinical applications and performance of laser speckle contrast imaging

et al. 2020

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<p class="abstract">Laser speckle contrast imaging (LSCI) is a useful tool for visualizing full-field blood flow images. Speckle pattern is formed when a coherent light illuminates a rough object, and the backscattered radiation is transformed into images and be shown on a screen. Movement within the object results in the fluctuation of patterns over time. The same data can be obtained by employing the Doppler effect, yet producing a two-dimensional Doppler map needs scanning; speckle imaging renders the same information without the requirement to scan. Nowadays, LSCI has gained expanded consideration, in part because of its accelerated adoption for blood flow studies in the different surgical departments. Here we represent and review the application of laser speckle contrast methods to the field of perfusion visualization as clinical studies from various medical fields and discuss the limitations hindering clinical acceptance.</p>

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Novel Optical Techniques for Imaging Microcirculation in the Diabetic Foot

Abstract: Although promising, the currently available novel optical techniques need to be further improved technically and prospective trials are necessary to evaluate their clinical value.

Cited by 31 publications

References 86 publications

Clinical applications of laser speckle contrast imaging: a review

Clinical applications of laser speckle contrast imaging: a review

In vivo label‐free functional photoacoustic monitoring of ischemic reperfusion

A review of the clinical applications and performance of laser speckle contrast imaging

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