Novel PGS/PCL electrospun fiber mats with patterned topographical features for cardiac patch applications

Tallawi, Marwa; Dippold, Dirk; Rai, Ranjana; D’Atri, Domenico; Roether, Judith A.; Schubert, Dirk W.; Rosellini, Elisabetta; Engel, Felix B.; Boccaccını, Aldo R.

doi:10.1016/j.msec.2016.06.083

Cited by 65 publications

(71 citation statements)

References 38 publications

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“…Alpha‐actinin is a major constituent of the contractile apparatus of mature cardiomyocytes involved in the thin filaments in the sarcomeres . As shown in Figure a, our results are consistent with previous studies on the expression levels of the sarcomeric units of alpha‐actinin on microscale anisotropically patterned materials . However, cardiomyoblasts grown on the Align‐HG scaffold did not show the distinguishing features of cardiomyocytes and even showed a disorganized pattern of the multinucleated myotubes.…”

Section: Discussionsupporting

confidence: 91%

Effect of spatial arrangement and structure of hierarchically patterned fibrous scaffolds generated by a femtosecond laser on cardiomyoblast behavior

Jun

Kim

Chung

et al. 2018

J Biomedical Materials Res

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Biological responses on biomaterials occur either on their surface or at the interface. Therefore, surface characterization is an essential step in the fabrication of ideal biomaterials for achieving effective control of the interaction between the material surface and the biological environment. Herein, we applied femtosecond laser ablation on electrospun fibrous scaffolds to fabricate various hierarchical patterns with a focus on the alignment of cells. We investigated the simultaneously stimulated response of cardiomyoblasts based on multiple topographical cues, including scales, oriented directions, and spatial arrangements, in the fibrous scaffolds. Our results demonstrated a synergistic effect on cell behaviors of one or more structural arrangements in a homogeneous orientation, whereas antagonistic effects were observed for cells arranged on a surface with heterogeneous directions. Taken together, these results indicate that our hierarchically patterned fibrous scaffolds may be useful tools for understanding the cellular behavior on fibrous scaffolds used to mimic an extracellular matrix-like environment. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1732-1742, 2018.

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Section: Discussionsupporting

confidence: 91%

Effect of spatial arrangement and structure of hierarchically patterned fibrous scaffolds generated by a femtosecond laser on cardiomyoblast behavior

Jun

Kim

Chung

et al. 2018

J Biomedical Materials Res

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“…Moreover, the composition of these cardiac patches should be based on biocompatible materials that can also be biodegraded in a clinically relevant time frame. Recent advancements in the field of material chemistry and microfabrication have allowed the engineering of a variety of cell-laden and acellular cardiac patches, which are based on both synthetic and naturally-derived biomaterials [12,[15][16][17][18][19][20]. However, since electromechanical coupling is essential for the contractile function of the heart, alternative strategies to restore electrical conductivity at the site of MI should also be investigated [21].…”

Section: Introductionmentioning

confidence: 99%

Engineering a naturally-derived adhesive and conductive cardiopatch

et al. 2019

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Myocardial infarction (MI) leads to a multi-phase reparative process at the site of damaged heart that ultimately results in the formation of non-conductive fibrous scar tissue. Despite the widespread use of electroconductive biomaterials to increase the physiological relevance of bioengineered cardiac tissues in vitro, there are still several limitations associated with engineering biocompatible scaffolds with appropriate mechanical properties and electroconductivity for cardiac tissue regeneration. Here, we introduce highly adhesive fibrous scaffolds engineered by electrospinning of gelatin methacryloyl (GelMA) followed by the conjugation of a choline-based bio-ionic liquid (Bio-IL) to develop conductive and adhesive cardiopatches. These GelMA/Bio-IL adhesive patches were optimized to exhibit mechanical and conductive properties similar to the native myocardium. Furthermore, the engineered patches strongly adhered to murine myocardium due to the formation of ionic bonding between the Bio-IL and native tissue, eliminating the need for suturing. Co-cultures of primary cardiomyocytes and cardiac fibroblasts grown on GelMA/Bio-IL patches exhibited comparatively better contractile profiles compared to pristine GelMA controls, as demonstrated by over-expression of the gap junction protein connexin 43. These cardiopatches could be used to provide mechanical support and restore electromechanical coupling at the site of MI to minimize cardiac remodeling and preserve normal cardiac function.

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“…Many types of materials including natural and synthetic polymers have been used to fabricate cardiac patches with different properties . Although natural polymers lack appropriate mechanical properties, they do promote cellular functions such as attachment, proliferation, and differentiation, as well as having tunable degradation with nontoxic degradation products .…”

Section: Introductionmentioning

confidence: 99%

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

Norahan

Amroon

Ghahremanzadeh

et al. 2018

J Biomedical Materials Res

104

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The application of a cardiac patch over the epicardial surface has shown positive effects in protecting cardiac function postinfarction. Electroactive patches could enhance electrical signal propagation among cardiac cells. In the present study, an electrically active composite of collagen and graphene oxide (Col-GO) was fabricated as a cardiac patch. Col scaffolds were fabricated using a freeze-drying method and coated covalently with GO. Some scaffolds were also reduced by a reduction agent to restore the high conductivity of GO. GO was shown to be a single layer with suitable lateral size for biological application. The Col-GO scaffolds contained randomly oriented interconnected pores with appropriate pore sizes of 120-138 AE 8 μm. GO flakes were also well distributed in the pore walls. By increasing the GO concentration, the tensile strength of the scaffolds was enhanced from 75 kPa for Col-GO-5 to 162 kPa for Col-GO-90. Young modulus also followed the same trend. Electrical conductivity of the scaffolds was in the range of semi-conductive materials (~10 −4 S/m), which is suitable for cardiac tissue engineering applications. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated no toxic effects on human umbilical vein endothelial cells (HUVECs) after 96 h. Also, a 10-days degradation product of the samples was compatible with HUVECs. Reduced scaffolds supported neonatal cardiomyocyte adhesion and upregulated the expression of the cardiac genes, including Cx43, Actin4, and Trpt-2 than their nonconductive counterparts. The obtained results confirmed the angiogenic properties of reduced Go-containing materials for cardiovascular applications where angiogenesis plays an important role, especially postinfarction.

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Novel PGS/PCL electrospun fiber mats with patterned topographical features for cardiac patch applications

Cited by 65 publications

References 38 publications

Effect of spatial arrangement and structure of hierarchically patterned fibrous scaffolds generated by a femtosecond laser on cardiomyoblast behavior

Effect of spatial arrangement and structure of hierarchically patterned fibrous scaffolds generated by a femtosecond laser on cardiomyoblast behavior

Engineering a naturally-derived adhesive and conductive cardiopatch

Electroactive graphene oxide‐incorporated collagen assisting vascularization for cardiac tissue engineering

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