Novel Point Mutations in Mitochondrial MT-CO2 Gene May Be Risk Factors for Coronary Artery Disease

Heidari, Mohammad; Mirfakhradini, Fatemeh Sadat; Tayefi, Fahime; Ghorbani, Saghar; Khatami, Mehri; Hadadzadeh, Mehdi

doi:10.1007/s12010-020-03275-0

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…A comparison of amino acids between AG1-IA cox2 and human cox2 (i.e., MT-CO2 ) showed highly conserved sequences, with E -value of 2e-72 and identity of 46%. The sequence mutations in human MT-CO2 have been reported to be related to serious diseases ( Rahman et al, 1999 ; Heidari et al, 2020 ), suggesting that there may be also some potential key pathogenic factors in the R. solani mitogenome. The CRISPR gene-editing technology could facilitate genetic alterations in fungal genomes and enable study of gene function ( Liu et al, 2015 ; Muñoz et al, 2019 ), in relation to changes in fungal growth, morphology, and virulence.…”

Section: Discussionmentioning

confidence: 99%

Comparative Mitogenomic Analysis and the Evolution of Rhizoctonia solani Anastomosis Groups

et al. 2021

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Mitochondria are the major energy source for cell functions. However, for the plant fungal pathogens, mitogenome variations and their roles during the host infection processes remain largely unknown. Rhizoctonia solani, an important soil-borne pathogen, forms different anastomosis groups (AGs) and adapts to a broad range of hosts in nature. Here, we reported three complete mitogenomes of AG1-IA RSIA1, AG1-IB RSIB1, and AG1-IC, and performed a comparative analysis with nine published Rhizoctonia mitogenomes (AG1-IA XN, AG1-IB 7/3/14, AG3, AG4, and five Rhizoctonia sp. mitogenomes). These mitogenomes encoded 15 typical proteins (cox1-3, cob, atp6, atp8-9, nad1-6, nad4L, and rps3) and several LAGLIDADG/GIY-YIG endonucleases with sizes ranging from 109,017 bp (Rhizoctonia sp. SM) to 235,849 bp (AG3). We found that their large sizes were mainly contributed by repeat sequences and genes encoding endonucleases. We identified the complete sequence of the rps3 gene in 10 Rhizoctonia mitogenomes, which contained 14 positively selected sites. Moreover, we inferred a robust maximum-likelihood phylogeny of 32 Basidiomycota mitogenomes, representing that seven R. solani and other five Rhizoctonia sp. lineages formed two parallel branches in Agaricomycotina. The comparative analysis showed that mitogenomes of Basidiomycota pathogens had high GC content and mitogenomes of R. solani had high repeat content. Compared to other strains, the AG1-IC strain had low substitution rates, which may affect its mitochondrial phylogenetic placement in the R. solani clade. Additionally, with the published RNA-seq data, we investigated gene expression patterns from different AGs during host infection stages. The expressed genes from AG1-IA (host: rice) and AG3 (host: potato) mainly formed four groups by k-mean partitioning analysis. However, conserved genes represented varied expression patterns, and only the patterns of rps3-nad2 and nad1-m3g18/mag28 (an LAGLIDADG endonuclease) were conserved in AG1-IA and AG3 as shown by the correlation coefficient analysis, suggesting regulation of gene repertoires adapting to infect varied hosts. The results of variations in mitogenome characteristics and the gene substitution rates and expression patterns may provide insights into the evolution of R. solani mitogenomes.

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Section: Discussionmentioning

confidence: 99%

Comparative Mitogenomic Analysis and the Evolution of Rhizoctonia solani Anastomosis Groups

et al. 2021

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“…In addition, MTND5 can also catalyze NADH to transfer electrons through the respiratory chain to produce energy (Su et al, 2020). MT-CYB is a component of the ubiquinolcytochrome C reductase complex, which mediates electron transfer from ubiquinol to cytochrome C. This contributes to the generation of a proton gradient across the mitochondrial membrane, which is then used for ATP synthesis, thereby playing an important role in energy generation (Heidari et al, 2020). but also may lead to more ROS production, resulting in changes in the bone homeostasis environment (Li et al, 2017).…”

Section: Bioinformatics Analysismentioning

confidence: 99%

TMT‐based proteomics analysis to screen potential biomarkers of Achyranthis Bidentatae Radix for osteoporosis in rats

Yang

Hou

Zhang

et al. 2022

Biomedical Chromatography

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This study aimed to explore the possible mechanism of Achyranthis Bidentatae Radix for the treatment of osteoporosis using tandem mass tag-based proteomics technique combined with mass spectrometry. Proteomics techniques combined with bioinformatics were used to analyze the biological functions of differentially expressed proteins. In addition, western blotting was performed to verify the expression of related proteins. A total of 3,752 proteins were identifiable by proteomic analysis. Furthermore, 93 differentially expressed proteins were identified, of which 61 were upregulated and 32 were downregulated. Differentially expressed proteins were primarily associated with oxidative phosphorylation (p = 4.8 Â 10 À4 ) pathways and involved in transmembrane transport (p = 3.5 Â 10 À3 ), exocytic process (p = 1.2 Â 10 À2 ), cellular developmental process (p = 1.3 Â 10 À2 ), adenosine triphosphate metabolic process (p = 1.0 Â 10 À2 ) and other biological processes. Western blotting analysis showed that MT-CYB and NDUFA9 were differentially expressed in the bone microenvironment of rats with osteoporosis. We speculated that they were potential biomarkers linked to osteoporosis. This study employed proteomics to explore the potential therapeutic targets of Achyranthis Bidentatae Radix to treat osteoporosis. This revealed that mitochondria are a new target for the treatment of glucocorticoid-induced osteoporosis.

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“…It can be inferred from our results that MT-Cox2 may play a crucial role in the process of inflammatory cytokines expression and MRC dysfunction in HAPE. Cox subunit 2 (Cox2) is an integral component of Cox, which is closely related to the mitochondrial electron transfer and ATP synthesis (Heidari et al, 2020). A previous experiment demonstrated that Cox2 was significantly decreased in some patients whose Cox function was deficient (Brischigliaro and Zeviani, 2021) and in the ischemia-reperfusion animal models (Mariero et al, 2019).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Effects of Plateau Air Quality and Low Oxygen Content on Platelet mtDNA Methylation and High Altitude Pulmonary Edema

Liu

Cui

Zhao

et al. 2021

Aerosol Air Qual. Res.

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Novel Point Mutations in Mitochondrial MT-CO2 Gene May Be Risk Factors for Coronary Artery Disease

Cited by 13 publications

References 37 publications

Comparative Mitogenomic Analysis and the Evolution of Rhizoctonia solani Anastomosis Groups

Comparative Mitogenomic Analysis and the Evolution of Rhizoctonia solani Anastomosis Groups

TMT‐based proteomics analysis to screen potential biomarkers of Achyranthis Bidentatae Radix for osteoporosis in rats

Effects of Plateau Air Quality and Low Oxygen Content on Platelet mtDNA Methylation and High Altitude Pulmonary Edema

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