Novel quantitative method for determination of molecular species of phospholipids and diglycerides

Blank, Merle L.; Robinson, Mitchell; Fitzgerald, Veronica; Snyder, Fred

doi:10.1016/s0021-9673(01)92744-x

Cited by 263 publications

(63 citation statements)

References 17 publications

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“…The lipid composition and major molecular species of glycerophospholipids of human red blood cells were previously rigorously characterized (38) by a sophisticated analytical routine that involved several TLC separations, hydrolysis of lipids by phospholipase C, and derivatization of diradylglycerols by benzoic anhydride followed by quantification of diradylglycerolbenzoates by reversed-phase HPLC (39). Diradylglycerolbenzoates were separated into 20 chromatographic peaks (some of which contained a few coeluted species); however, separation of positional isomers was not achieved (39).…”

Section: Validation Of the Mass Spectrometric Approachmentioning

confidence: 99%

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Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation

Ekroos

Ejsing

Bahr

et al. 2003

Journal of Lipid Research

292

317

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Section: Validation Of the Mass Spectrometric Approachmentioning

confidence: 99%

“…Diradylglycerolbenzoates were separated into 20 chromatographic peaks (some of which contained a few coeluted species); however, separation of positional isomers was not achieved (39).…”

Section: Validation Of the Mass Spectrometric Approachmentioning

confidence: 99%

Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation

Ekroos

Ejsing

Bahr

et al. 2003

Journal of Lipid Research

292

317

View full text Add to dashboard Cite

“…Each phospholipid class was initially hydrolyzed by phospholipase C treatment and converted to benzoate derivatives as previously described (44 -46). The resultant diradylglycerobenzoates were extracted and separated on silica gel 60 G plates using benzene/hexane/diethyl ether (50:45:4, v/v/v) as the developing solvent (45). The alkenylacyl, alkylacyl, and diacylglycerobenzoates were extracted using ethanol/water/hexane (1:1:1, v/v/v) (44).…”

Section: Isolation Of Lipid Raft Glycerophosphocholine (Gpc) and Glycmentioning

confidence: 99%

Dietary Docosahexaenoic Acid Suppresses T Cell Protein Kinase Cθ Lipid Raft Recruitment and IL-2 Production

Yang¹,

Ly²,

Barhoumi

et al. 2004

The Journal of Immunology

222

173

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To date, the proximal molecular targets through which dietary n-3 polyunsaturated fatty acids (PUFA) suppress the inflammatory process have not been elucidated. Because cholesterol and sphingolipid-enriched rafts have been proposed as platforms for compartmentalizing dynamically regulated signaling assemblies at the plasma membrane, we determined the in vivo effects of fish oil and highly purified docosahexaenoic acid (DHA; 22:6n-3) on T cell microdomain lipid composition and the membrane subdomain distribution of signal-transducing molecules (protein kinase C (PKC)θ, linker for activation of T cells, and Fas/CD95), before and after stimulation. Mice were fed diets containing 5 g/100 g corn oil (control), 4 g/100 g fish oil (contains a mixture of n-3 PUFA) plus 1 g/100 g corn oil, or 4 g/100 g corn oil plus 1 g/100 g DHA ethyl ester for 14 days. Dietary n-3 PUFA were incorporated into splenic T cell lipid raft and soluble membrane phospholipids, resulting in a 30% reduction in raft sphingomyelin content. In addition, polyclonal activation-induced colocalization of PKCθ with lipid rafts was reduced by n-3 PUFA feeding. With respect to PKCθ effector pathway signaling, both AP-1 and NF-κB activation, IL-2 secretion, and lymphoproliferation were inhibited by fish oil feeding. Similar results were obtained when purified DHA was fed. These data demonstrate for the first time that dietary DHA alters T cell membrane microdomain composition and suppresses the PKCθ signaling axis.

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“…The diacylglycerol benzoates were analyzed by HPLC on a Model LC-6A apparatus (Shimadzu), equipped with an ODS column (4.6×250 mm, Lichrosorb RP-18; E. Merck), by the method of Blank et al 11) Liver microsomal protein was measured as described by Lowry et al 12) Results were evaluated by one-way analysis of variance, and the diŠer-ence between means was examined by Duncan's multiple range test. 13) In some cases, Student's t-test was used to examine the signiˆcance of diŠerences between the two experimental groups.…”

Section: )mentioning

confidence: 99%

Effects ofLentinus edodeson Fatty Acid and Molecular Species Profiles of Phosphatidylcholine in Rats Fed Different Levels of Corn Oil

Shimada

Morita

Sugiyama

2002

Bioscience, Biotechnology, and Biochemistry

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Shiitake mushrooms (Lentinus edodes) are a hypocholesterolemic and aŠect phospholipid and fatty acid metabolism in rats. In this study, the eŠects of 2% shiitake in the diet on fatty acid and molecular species proˆles of liver microsomal and plasma phosphatidylcholine (PC) were investigated in rats fed diets containing diŠerent levels (1-20%) of corn oil, a linoleic-acidrich fat. The proportion of 18:2n-6 in PC increased depending on the parcent corn oil, and L. edodes further increased the proportion at all corn oil levels. The proportion of 20:4n-6 was lower in rats fed L. edodes than in rats fed control diets irrespective of the parcent corn oil. L. edodes selectively increased the proportion of 16:0-18:2 molecular species and decreased the proportion of 18:0-20:4 molecular species in PC. These results indicate that the eŠects of L. edodes on fatty acid and molecular species proˆles of PC are stronger than that of the dietary corn oil level.

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Novel quantitative method for determination of molecular species of phospholipids and diglycerides

Cited by 263 publications

References 17 publications

Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation

Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation

Dietary Docosahexaenoic Acid Suppresses T Cell Protein Kinase Cθ Lipid Raft Recruitment and IL-2 Production

Effects ofLentinus edodeson Fatty Acid and Molecular Species Profiles of Phosphatidylcholine in Rats Fed Different Levels of Corn Oil

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