Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes

Balanč, Bojana D.; Trifković, Kata; Đorđević, Verica; Marković, Smilja; Pjanović, Rada; Nedović, Viktor; Bugarski, Branko

doi:10.1016/j.foodhyd.2016.07.005

Cited by 74 publications

(38 citation statements)

References 60 publications

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“…By increasing the phospholipid concentration, the surface charge of the particles decreased. The negative surface charge which the liposomes exhibited is due to the type of structural phospholipids and, according to the literature, is consistent with the liposomes prepared with phosphatidylcholine 43. These findings are in agreement with those reported by others 55,57.…”

Section: Resultssupporting

confidence: 92%

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Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Tefas

Sylvester

Tomuță

et al. 2017

DDDT

115

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The aim of this work was to use the quality-by-design (QbD) approach in the development of long-circulating liposomes co-loaded with curcumin (CUR) and doxorubicin (DOX) and to evaluate the cytotoxic potential of these liposomes in vitro using C26 murine colon carcinoma cell line. Based on a risk assessment, six parameters, namely the phospholipid, CUR and DOX concentrations, the phospholipid:cholesterol molar ratio, the temperature during the evaporation and hydration steps and the pH of the phosphate buffer, were identified as potential risk factors for the quality of the final product. The influence of these variables on the critical quality attributes of the co-loaded liposomal CUR and DOX was investigated: particle size, zeta potential, drug loading and entrapment efficiency. For this, a 26−2 factorial design was employed to establish a proper regression model and to generate the contour plots for the responses. The obtained data served to establish the design space for which different combinations of variables yielded liposomes with characteristics within predefined specifications. The validation of the model was carried out by preparing two liposomal formulations corresponding to the robust set point from within the design space and one outside the design space and calculating the percentage bias between the predicted and actual experimental results. The in vitro antiproliferative test showed that at higher CUR concentrations, the liposomes co-encapsulating CUR and DOX had a greater cytotoxic effect than DOX-loaded liposomes. Overall, this study showed that QbD is a useful instrument for controlling and optimizing the manufacturing process of liposomes co-loaded with CUR and DOX and that this nanoparticulate system possesses a great potential for use in colon cancer therapy.

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Section: Resultssupporting

confidence: 92%

“…It is agreed that particles can aggregate due to insufficient electric repulsion between them when the zeta potential values range between −30 and +30 mV 43,44. Thus, the zeta potential was chosen as a CQA of CUR-DOX-LCL.…”

Section: Resultsmentioning

confidence: 99%

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Tefas

Sylvester

Tomuță

et al. 2017

DDDT

115

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“…Prepared microbeads had negative values of zeta potential (-35.73 ± 0.15 mV), which indicates good electrostatic stabilization. According to the previously published research, microparticles can be considered as stabile ones when the absolute value of the zeta potential is around 30 mV due to the electric repulsion between the particles (Balanč et al, 2016). Results showed that obtained microparticles were stable, and particles repulsion was achieved, agglomeration was prevented which are exceptionally important parameters for potential powder application.…”

Section: Zeta Potentialmentioning

confidence: 95%

Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method

et al. 2018

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Due to its high content of phenolics, black chokeberry has been proposed as a health-promoting material, especially use of chokeberry extract as a food or pharmaceuticals. Chokeberry extract was spray-dried using gum Arabic as a carrier. The optimal conditions for the efficient microencapsulation of chokeberry extract using spray-drying technique have been investigated. The aim of our study was to obtain microparticles with the best potential to improve functionality and stability of extracted chokeberry polyphenols, and to investigate the possibility of microbeads to protect active compounds during simulated digestion process. Morphological characteristics of microbeads were analyzed using FTIR and SEM techniques. Zeta potential, particle size and moisture content were determined. Released total phenolics and total anthocyanins content as well as concentration of individual anthocyanins were quantified before and after digestion process. Microparticles exhibited high encapsulation efficiency up to 87%, and high content of released polyphenols was achieved. After in vitro simulated digestion phenolic compounds decreased by 11-24%, exhibited higher protective effect of gum Arabic. Our results showed that chokeberry microparticles obtained by spray drying method could be useful supplements or functional food.

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“…The liposome formulations had high negatively charged values ranging from -20.18 to -43.71 mV, due to the presence of surface charge in the formulations providing negative charge. These high zeta potential values (above -20 mV) increased the stability of vesicles, due to the electro static repulsion between the vesicles [19,20,28]. Although the zeta potential of some prepared liposomes was less than 30 mV, there was no precipitation.…”

Section: Liposome Formulation and Characterizationmentioning

confidence: 98%

Phenylethyl Resorcinol Loaded in Liposomal Cream Formulation for Cosmeceutical Application

Raknam

Pinsuwan

Amnuaikit

2020

JPRI

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Phenylethyl Resorcinol (PR) is a cosmeceutical skin lightening agent and the purpose of this study was to enhance its stability by using liposomal cream formulation which increases local efficacy and safety. Liposome formulation was prepared by modified ethanol injection method, and it contained soy phosphatidyl choline (SPC), cholesterol (CHO), Tween 80 (TW80) and deoxycholic acid (DA) mixed with 2% PR. The physicochemical properties, skin permeation as well as cellular study were evaluated in order to obtain the optimized formulation. The optimized liposome formulation composed of SPC:TW80:DA (84:16:2.5) and exhibited vesicle size, polydispersity index (PDI) and zeta potential of 286.4±8.04 nm, 0.317±0.03 and -39.20±3.85 mV, respectively. Entrapment efficiency (EE) of liposome formulation was 93.55±0.05%. The vesicle was spherical in shape and showed good physicochemical stability for 4 months. The skin permeation study demonstrated that liposome with a negative charge could result in a high PR skin deposition value of 1732.76±216.24 µg/cm2 after 24 h. Cellular study showed that liposome formulation could inhibit melanin content in B16 melanoma cells and enhance cell viability in HaCaT keratinocyte cells. The optimized PR liposome was incorporated in cream and investigated physicochemical properties, stability and skin permeation. Liposomal PR cream showed a good stability and a superior result than PR cream in skin permeation parameters, as well as in tyrosinase inhibition.

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Novel resveratrol delivery systems based on alginate-sucrose and alginate-chitosan microbeads containing liposomes

Cited by 74 publications

References 60 publications

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method

Phenylethyl Resorcinol Loaded in Liposomal Cream Formulation for Cosmeceutical Application

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