Novel sensing probe using Terbium-sensitized luminescence and 8-hydroxyquinoline for determination of prucalopride succinate: green assessment with Complex-GAPI and analytical Eco-Scale

Abstract

A highly sensitive spectrofluorimetric method is developed for the determination of prucalopride succinate (PRU). The method depends on lanthanide-sensitized luminescence due to complex formation between the drug and terbium chloride (Tb+3) which is enhanced by the addition of 8 hydroxyquinoline (8HQ) and phosphate buffer (0.02 M, pH 3.2). The calibration curve was constructed over the linear range 10–300 ng/mL after excitation at 226 nm and measuring the emission of the ternary complex at 544 nm. The method was validated according to ICH Q2 (R1) guidelines and showed a good recovery ± RSD of 100.41% ± 1.26, the limits of detection and quantitation were found to be 2.81 and 8.53 ng/mL, respectively. The proposed method was successfully applied for the determination of the drug marketed tablet dosage form and the results were in good agreement with the reference method. Also, the method greenness was evaluated according to Complex-GAPI and analytical Eco-Scale.

Integrated resonance Rayleigh scattering approach utilizing Box–Behnken experimental design for the facile quantification of prucalopride in pharmaceutical tablets and human urine with sustainability assessment

“…[14][15][16] The spectrophotometric methods suffered from limited sensitivity. While, the procedure of the rst spectrouorimetric method 14 was tedious, lengthy, utilized costly reagent (terbium chloride) and an environmental contaminating one (8-hydroxyquinoline). Although the second method 15 had simple procedure, its sensitivity was less than the proposed method.…”

Bioanalytical Validated Spectrofluorimetric Method for the Determination of Prucalopride succinate in Human Urine Samples and Its Greenness Evaluation

“…The literature review revealed the determination of PRU using various analytical techniques including chromatographic [4][5][6][7][8][9][10], spectrophotometric [11] methods and only one reported spectrofluorometric procedure that uses luminescence reaction [12] but there is no quantification in the urine sample. Spectrofluorimetric technique is commonly used in drugs analysis either in dosage form or in biological fluids for its high sensitivity and simplicity [13][14][15].…”

Sensitive spectrofluorimetric determination of the prokinetic drug prucalopride succinate based on supramolecular aggregation approach with evaluation of method greenness: application to content uniformity test

“…For PCP analysis, literature reports document few spectrophotometric methods [3][4][5][6][7], spectrofluorimetric methods [8][9][10][11], chromatographic methods [12][13][14][15], and electrochemical methods [16,17]. The reported spectrophotometric methods had limited sensitivity.…”

“…Four spectrofluorimetric methods were reported for PCP determination. However, the procedure of the first method involved the use of multiple reagents containing metal ions, buffers, and auxiliary chelating agents [8]. In addition, the reaction cocktail should be allowed to stand for at least 15 min before measuring the fluorescence of the formed product.…”