Novel SH-SAW Biosensors for Ultra-Fast Recognition of Growth Factors

Matatagui, Daniel; Bastida, Ágatha; Horrillo, M.C.

doi:10.3390/bios12010017

Cited by 9 publications

(5 citation statements)

References 38 publications

(39 reference statements)

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“…NT3 consists of 119 amino acids and possesses an IEP of 9.5 [ 268 ]. The kinetics of adsorption of NT3, from low bulk concentration (50 mg/mL), was successfully determined on a modified silica biosensor by Matatagui et al [ 269 ]. NT4/5 molecules form non-covalent homodimers.…”

Section: Macroionsmentioning

confidence: 99%

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

2023

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Growth factors are a class of proteins that play a role in the proliferation (the increase in the number of cells resulting from cell division) and differentiation (when a cell undergoes changes in gene expression becoming a more specific type of cell) of cells. They can have both positive (accelerating the normal healing process) and negative effects (causing cancer) on disease progression and have potential applications in gene therapy and wound healing. However, their short half-life, low stability, and susceptibility to degradation by enzymes at body temperature make them easily degradable in vivo. To improve their effectiveness and stability, growth factors require carriers for delivery that protect them from heat, pH changes, and proteolysis. These carriers should also be able to deliver the growth factors to their intended destination. This review focuses on the current scientific literature concerning the physicochemical properties (such as biocompatibility, high affinity for binding growth factors, improved bioactivity and stability of the growth factors, protection from heat, pH changes or appropriate electric charge for growth factor attachment via electrostatic interactions) of macroions, growth factors, and macroion-growth factor assemblies, as well as their potential uses in medicine (e.g., diabetic wound healing, tissue regeneration, and cancer therapy). Specific attention is given to three types of growth factors: vascular endothelial growth factors, human fibroblast growth factors, and neurotrophins, as well as selected biocompatible synthetic macroions (obtained through standard polymerization techniques) and polysaccharides (natural macroions composed of repeating monomeric units of monosaccharides). Understanding the mechanisms by which growth factors bind to potential carriers could lead to more effective delivery methods for these proteins, which are of significant interest in the diagnosis and treatment of neurodegenerative and civilization diseases, as well as in the healing of chronic wounds.

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Section: Macroionsmentioning

confidence: 99%

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

2023

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“…This was due to an increase in mass due to antibody-antigen affinity, which produced a change in surface acoustic wave rate and therefore a change in resonant frequency (Figure. 8c) 93 . Since d-amino acid levels are often associated with different neurological disorders, it is important to develop rapid and specific biosensors for the determination of d-serine.…”

Section: Detection Of Biomoleculesmentioning

confidence: 99%

“…8c). 93 Since d -amino acid levels are often associated with different neurological disorders, it is important to develop rapid and specific biosensors for the determination of d -serine. Pietrantonio et al 94 fabricated a SAW biosensor with a PLP-reduced d -serine dehydratase for the detection of d -serine levels (Fig.…”

Section: Real-time Biosensors Based On Sh-sawmentioning

confidence: 99%

Surface acoustic wave based microfluidic devices for biological applications

et al. 2023

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“…The acoustic wave parameters, typically amplitude and/or phase, shift because of specific biomolecules' presence on the acoustic wave-guide. Matatagui et al [ 50 ] demonstrated that SAW is a very fast, sensitive and label-free detection method for the grow factor detection.…”

Section: Biosensor Technologymentioning

confidence: 99%

Device Processing Challenges for Miniaturized Sensing Systems Targeting Biological Fluids

Stoukatch

Dupont

Redouté

2022

Biomedical Materials & Devices

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This article presents a review of device processing technologies used in the fabrication of biomedical systems, and highlights the requirements of advanced manufacturing technology. We focus on biomedical systems that perform diagnostics of fluidic specimens, with analytes that are in the liquid phase. In the introduction, we define biomedical systems as well as their versatile applications and the essential current trends. The paper gives an overview of the most important biomolecules that typically must be detected or analyzed in several applications. The paper is structured as follows. First, the conventional architecture and construction of a biosensing system is introduced. We provide an overview of the most common biosensing methods that are currently used for the detection of biomolecules and its analysis. We present an overview of reported biochips, and explain the technology of biofunctionalization and detection principles, including their corresponding advantages and disadvantages. Next, we introduce microfluidics as a method for delivery of the specimen to the biochip sensing area. A special focus lies on material requirements and on manufacturing technology for fabricating microfluidic systems, both for niche and mass-scale production segments. We formulate requirements and constraints for integrating the biochips and microfluidic systems. The possible impacts of the conventional microassembly techniques and processing methods on the entire biomedical system and its specific parts are also described. On that basis, we explain the need for alternative microassembly technologies to enable the integration of biochips and microfluidic systems into fully functional systems.

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Novel SH-SAW Biosensors for Ultra-Fast Recognition of Growth Factors

Cited by 9 publications

References 38 publications

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

Biocompatible Macroion/Growth Factor Assemblies for Medical Applications

Surface acoustic wave based microfluidic devices for biological applications

Device Processing Challenges for Miniaturized Sensing Systems Targeting Biological Fluids

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