Novel single-cell functional analysis of red blood cells using laser tweezers Raman spectroscopy: Application for sickle cell disease

Li, Rui; Mao, Ziliang; Matthews, Dennis L; Li, Chin‐Shang; Chan, James W.; Satake, Noriko

doi:10.1016/j.exphem.2013.02.012

Cited by 41 publications

(27 citation statements)

References 14 publications

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“…70 Workers at the Center for Biophotonics, Science and Technology, UC Davis, who contributed to developing the LTRS capability, have studied a number of these phenomena. [71][72][73] Red Blood Cell Membrane. As some investigators were elucidating the form and function of RBC hemoglobin, other groups were characterizing RBC membranes.…”

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“…40 In 2012, the UC Davis group (also mentioned above) used sickle cells as demonstration samples in one of the early reports of LTRS, 72 and they later used the technique to show that sickle cells deoxygenated more readily under (optical or mechanical) stress than healthy control cells. 73 Recently, Raman spectroscopy has been proposed as a tool for quick and accurate diagnosis of SCD, and a clinical investigation has been performed by researchers from Brazil. The results showed that subtle changes in the polypeptide chain of abnormal hemoglobin could be discerned from the normal hemoglobin variant, and that the chemometric model designed by the group correctly discriminated 100% of the samples in each cell class.…”

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Raman Spectroscopy of Blood and Blood Components

et al. 2017

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Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys. Measured levels of oxygen, nutrients, waste, and electrolytes in blood are often used for clinical assessment of human health. Raman spectroscopy is a nondestructive analytical technique that uses the inelastic scattering of light to provide information on chemical composition, and hence has a potential role in this clinical assessment process. Raman spectroscopic probing of blood components and of whole blood has been on-going for more than four decades and has proven useful in applications ranging from the understanding of hemoglobin oxygenation, to the discrimination of cancerous cells from healthy lymphocytes, and the forensic investigation of crime scenes. In this paper, we review the literature in the field, collate the published Raman spectroscopy studies of erythrocytes, leucocytes, platelets, plasma, and whole blood, and attempt to draw general conclusions on the state of the field.

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Raman Spectroscopy of Blood and Blood Components

et al. 2017

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“…Compared to conventional thalassemia screening methods including Fourier-transform infrared (FT-IR) spectroscopy that require strict laboratory preparations and involve unwanted hemoglobin structural changes, Raman tweezers not only provide a simple, noninvasive and easy-to-implement alternative for thalassemia diagnosis, but also help with the investigation of the origin of the ineffective erythropoiesis in β-thalassemia. In addition to cell immobilization, OTs can apply adjustable optical forces on the trapped cells to study the degree of deoxygenation induced by chemical or mechanical pressure (known as mechanochemical phenomenon) from the variations in the spectral intensity of several oxygenation-specific Raman peaks [116][117][118]. Other investigations including ABO blood typing [119] and malaria-infected blood sample characterization [120] have been performed with Raman tweezers.…”

Section: Applications Of Raman Tweezersmentioning

confidence: 99%

Optical Tweezers in Studies of Red Blood Cells

Zhu

Avsievich

Попов

et al. 2020

Cells

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Optical tweezers (OTs) are innovative instruments utilized for the manipulation of microscopic biological objects of interest. Rapid improvements in precision and degree of freedom of multichannel and multifunctional OTs have ushered in a new era of studies in basic physical and chemical properties of living tissues and unknown biomechanics in biological processes. Nowadays, OTs are used extensively for studying living cells and have initiated far-reaching influence in various fundamental studies in life sciences. There is also a high potential for using OTs in haemorheology, investigations of blood microcirculation and the mutual interplay of blood cells. In fact, in spite of their great promise in the application of OTs-based approaches for the study of blood, cell formation and maturation in erythropoiesis have not been fully explored. In this review, the background of OTs, their state-of-the-art applications in exploring single-cell level characteristics and bio-rheological properties of mature red blood cells (RBCs) as well as the OTs-assisted studies on erythropoiesis are summarized and presented. The advance developments and future perspectives of the OTs’ application in haemorheology both for fundamental and practical in-depth studies of RBCs formation, functional diagnostics and therapeutic needs are highlighted.

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“…24 Raman tweezers have already been used for investigating red blood cell stress due to silver nanoparticles, stress due to glucose and in various human disorders such as thalassemia, sickle cell anemia, malaria etc. [25][26][27][28][29][30] Approximately 168 000 tons of thermal paper is annually utilized in European Community and these papers are reported to contain 1890 tons of BPA. 31 In the present work, we investigated the inuence of BPA (extracted from thermal paper) on live, human red blood cell using micro-Raman spectroscopy and optical trapping technique.…”

Section: Introductionmentioning

confidence: 99%