Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Fluorescence Quenching of Rhodamine B

Abstract

A novel, simple and specific spectrofluorimetric method was developed and validated for the determination of perindopril erbumine (PDE). The method is based on the fluorescence quenching of Rhodamine B upon adding perindopril erbumine. The quenched fluorescence was monitored at 578 nm after excitation at 500 nm. The optimization of the reaction conditions such as the solvent, reagent concentration, and reaction time were investigated. Under the optimum conditions, the fluorescence quenching was linear over a concentration range of 1.0-6.0 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the developed and reference methods revealed no significant differences between the methods compared in terms of accuracy and precision. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. The mechanism of rhodamine B quenching was also discussed.

Spectrofluorimetric Determination of Some N. Containing Medicines Using Rhodamine 6G as a Chromogenic Reagent

“…When the above drugs are added to the dye, a significant quenching of fluorescence intensity has been observed, and increased in an acidic medium has occurred. This may be due to the formation of non-fluorescent ion-pair complexes by electrostatic attraction between medicines and the dye [37,[43][44][45]. The decrease of fluorescence intensity of R-6G is found to be a linear function of Ncontaining medicines concentrations in water solution.…”

Metal–Dielectric Interfacial Engineering with Mesoporous Nano-Carbon Florets for 1000-Fold Fluorescence Enhancements: Smartphone-Enabled Visual Detection of Perindopril Erbumine at a Single-molecular Level

“…This choice of analyte is driven by (a) the importance of detecting PE at ultra-low concentrations due to its pharmacological relevance in the context of hypertension, post-myocardial infarction, and cardiovascular and cerebrovascular disease, 52−56 and (b) its synergistic and specific charge transfer-based interaction with RhB. 56 The 1000-fold SPCE enhancement afforded by AgNCF + GO is one of the highest reported using NCF substrates and is realized through rational synergistic engineering of metal-based plasmonics and dielectric-based EM hotspots with plasmons of extended π-conjugated frameworks (GO). Therefore, this SPCE platform was selected for the qualitative and quantitative detection of PE at ultra-low concentrations, extending to single-molecular limits.…”

“…Such synergistic coupling through multi-component integration lowers the Ohmic losses and contributes to unprecedented SPCE enhancements. Furthermore, the sharply directional, highly polarized, and enhanced emissions enable reliable and ultrasensitive detection of pharmacologically important drug (perindopril erbumine, PE) at single-molecular levels (0.1 attomolar) through a mobile phone-based platform, presenting an important capability for treatment of hypertension and myocardial infarction. − Thus, the materials and design principles illustrated in this report provide newer fundamental insights for achieving non-plasmonic and yet augmented SPCE enhancements, leading to versatile point-of-care diagnostic platforms.…”

Determination of perindopril erbumine by oxidative coupling reaction using 2, 6-dichloroquinone-4-chlorimide

“…Remington (Joseph, Alfonso, 2000) and physician's desk reference (Montvale, 2000). A survey of the literature revealed that UV (Nayak, Pillai, 2011;Kale et al, 2011), HPLC(El- Gizawy et al, 2014;Gizawy et al, 2013;Pattan, et al,2013;Singh et al, 2011), RP-HPLC (Chaudhary, Determination of perindopril erbumine by oxidative coupling reaction using 2, 6-dichloroquinone-4-chlorimide Patel, Chaudhary 2010;Mastannamma et al, 2016;Dugga, Peraman, Nayakanti, 2014;Joseph, Philip, 2011;Patel et al, 2011;Prajapati et al, 2011;Prameela Rani, Bala Sekaran, 2009), spectrofluorimetric (Fael Sakur 2015a. ;Fael Sakur 2015b), kinetic spectrophotometric (Rahman, Rahman, Haque, 2017;Rahman, Anwar, Kashif, 2006), LC-MS (Deepak, et al,2006) and GC-MS (Maurer, Kraemer, Arlt,1998) methods were reported for the estimation of PPE.…”