Novel spectrofluorimetric technique for determination of amoxicillin and ethopabate in chicken tissues, liver, kidney, eggs, and feed premix

Abstract

A new smart spectrofluorometric method was developed for the quantitation of amoxicillin and ethopabate simultaneously for the first time. The method is based on measuring their first derivative synchronous amplitudes in water at Δλ = 80 nm. The peak amplitudes were recorded at their crossing points; 240 nm for amoxicillin and 280 nm for ethopabate. The method is linear over the concentration ranges of 100.0-1,000.0 ng/ml for amoxicillin and 2.0-20.0 ng/ml for ethopabate. The limits of detection were 20.0 ng/ml and 0.58 ng/ml and limits of quantitation were 60.0 ng/ml and 1.92 ng/ml for amoxicillin and ethopabate, respectively. The method sensitivity permitted the determination of the two drugs below their maximum residue limit stated by the federal regulations. The developed method was applicable to the analysis of both drugs in the veterinary powders, feed premix, chicken tissues, liver, kidney, and eggs samples with percentage recoveries ranging 93.72-104.71%.

β‐MnOOH nanoplate‐enhanced chemiluminescence reaction and its application to the determination of amoxicillin and salbutamol sulfate

“…Results acquired were compared with previous methods suggested for determining AMX and SLB and shown in Table 1. For AMX, the LOD of our CL method was lower than that from HPLC, [ 14 ] is almost comparable with that of CE [ 18 ] and is higher than those of voltammetry, [ 15 ] colorimetry, [ 16 ] CL, [ 17 ] spectrofluorimetry, [ 19 ] and ECL [ 20 ] methods. For SLB, the LOD of the proposed CL method was lower than QCM [ 28 ] and comparable with voltammetry, [ 24 ] potentiometry, [ 25 ] CE, [ 27 ] and higher than spectrophotometry, [ 23 ] HPLC, [ 26 ] and spectrofluorimetry.…”

“…Results acquired were compared with previous methods suggested for determining AMX and SLB and shown in Table 1. For AMX, the LOD of our CL method was lower than that from HPLC, [14] is almost comparable with that of CE [18] and is higher than those of voltammetry, [15] colorimetry, [16] CL, [17] spectrofluorimetry, [19] and ECL [20] methods.…”

“…Amoxicillin (AMX) is a β‐lactam antibiotic that is used to treat strep throat and pneumonia and various types of skin, middle ear, and urinary tract infections, among others. [ 13 ] So far, various approaches have been proposed for determining AMX, including high performance liquid chromatography (HPLC), [ 14 ] voltammetry, [ 15 ] colorimetry, [ 16 ] CL, [ 17 ] capillary electrophoresis (CE), [ 18 ] spectrofluorimetry, [ 19 ] and electrochemiluminescence (ECL), [ 20 ] to name but a few.…”

Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma

“…This overlaying difficulty might be confronted by the employing synchronous technique, as it has several of benefits over standard fluorescence techniques, which include high selectivity, proper spectra, and minimum intrusion [34][35][36] . SFS is highly basic but proved to be an effective approach for the quantitative assessment of the two drugs in an appropriate time frame because of its narrow and sharp spectra [37][38][39][40] . REM and SIM emit separable synchronous…”

Spectrophotometric determination of amoxicillin in pharmaceutical formulations using normal and reverse flow injection analysis systems: A comparison study

“…Despite these advantages, concerns regarding AMX residues in food and the possible environmental contamination risk associated with this antibiotic have been raised due to its side effects on human health [4]. The literature covered a variety of analytical techniques [5] for the extraction and determination of AMX, including spectrofluorometry [6,7], electrochemistry [8][9][10], liquid chromatography-mass spectroscopy [11][12][13], highperformance thin layer chromatography (HPTLC) [14], high pressurized liquid chromatography (HPLC) [15], FIA [16][17][18][19][20], magnetic molecularly imprinted polymer [21], and spectrophotometry [22][23][24]. Flow-injection analysis (FIA) techniques are frequently utilized and have generated great interest because of their reproducibility, simplicity, and low apparatus costs [25][26][27][28][29].…”