Novel xanthine oxidase-based cell model using HK-2 cell for screening antihyperuricemic functional compounds

Hou, Chuanli; Liú, Dan; Wang, Ming; Gong, Congcong; Li, Yujuan; Yang, Liu; Yao, Maojin; Yuan, Erdong; Ren, Jiaoyan

doi:10.1016/j.freeradbiomed.2019.04.007

Cited by 45 publications

(30 citation statements)

References 29 publications

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“…The gut microbiota is established during the development of the host, and its composition and metabolism are closely associated with chronic metabolic syndromes. 29,30 Although various studies have shown that antihyperuricemia effects of protein hydrolysates in cell and mice models, 7,31 the underlying mechanism and the modulation effects of the protein hydrolysates on gut microbiota, remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

Han

Tang

et al. 2020

The FASEB Journal

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Recently, interest in using whole food‐derived mixtures to alleviate chronic metabolic syndrome through potential synergistic interactions among different components is increasing. In this study, the effects and mechanisms of tuna meat oligopeptides (TMOP) on hyperuricemia and associated renal inflammation were investigated in mice. Dietary administration of TMOP alleviated hyperuricemia and renal inflammation phenotypes, reprogramed uric acid metabolism pathways, inhibited the activation of NLRP3 inflammasome and TLR4/MyD88/NF‐κB signaling pathways, and suppressed the phosphorylation of p65‐NF‐κB. In addition, TMOP treatments repaired the intestinal epithelial barrier, reversed the gut microbiota dysbiosis and increased the production of short‐chain fatty acids. Moreover, the antihyperuricemia effects of TMOP were transmissible by transplanting the fecal microbiota from TMOP‐treated mice, indicating that the protective effects were at least partially mediated by the gut microbiota. Thus, for the first time, we clarify the potential effects of TMOP as a whole food derived ingredient on alleviating hyperuricemia and renal inflammation in mice, and additional efforts are needed to confirm the beneficial effects of TMOP on humans.

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Section: Discussionmentioning

confidence: 99%

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

Han

Tang

et al. 2020

The FASEB Journal

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“…The purine contents were determined by LC-20A HPLC system (Shimadzu, Kyoto, Japan) equipped with a variable-wavelength ultra-violet (UV) detector and an automatic injector according to the previous method ( Hou et al, 2019 ), with some modifications. In short, the HPLC separations of samples were conducted on a Microsorb-MV C18 column (150 mm × 4.60 mm,5 μm) at 254 nm with keeping 35 °C of the column.…”

Section: Methodsmentioning

confidence: 99%

Effect on purine releasement of Lentinus edodes by different food processing techniques

et al. 2022

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“…Human renal proximal tubule epithelial cell line (HK-2) was maintained in RPMI-1640 medium with 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin ( Hou et al, 2019 ) in a humidified incubator under an atmosphere of 95% air and 5% CO 2 for further study. The medium was changed every other day.…”

Section: Methodsmentioning

confidence: 99%

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

et al. 2021

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Background: The prevalence of hyperuricemia is considered high worldwide. Hyperuricemia occurs due to decreased excretion of uric acid, increased synthesis of uric acid, or a combination of both mechanisms. There is growing evidence that hyperuricemia is associated with a decline of renal function.Purpose: This study is aimed at investigating the effects of the novel compound on lowering the serum uric acid level and alleviating renal inflammation induced by high uric acid in hyperuricemic mice.Methods: Hyperuricemic mice model was induced by potassium oxonate and used to evaluate the effects of the novel compound named FxUD. Enzyme-linked immunosorbent assay was used to detect the related biochemical markers. Hematoxylin-eosin (HE) staining was applied to observe pathological changes. The mRNA expression levels were tested by qRT-PCR. The protein levels were determined by Western blot. In parallel, human proximal renal tubular epithelial cells (HK-2) derived from normal kidney was used to further validate the anti-inflammatory effects in vitro.Results: FxUD administration significantly decreased serum uric acid levels, restored the kidney function parameters, and improved the renal pathological injury. Meanwhile, treatment with FxUD effectively inhibited serum and liver xanthine oxidase (XOD) levels. Reversed expression alterations of renal inflammatory cytokines, urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) were observed in hyperuricemic mice. Western blot results illustrated FxUD down-regulated protein levels of inflammasome components. Further studies showed that FxUD inhibited the activation of NF-κB signaling pathway in the kidney of hyperuricemic mice. In parallel, the anti-inflammatory effect of FxUD was also confirmed in HK-2.Conclusion: Our study reveals that FxUD exhibits the anti-hyperuricemic and anti-inflammatory effects through regulating hepatic XOD and renal urate reabsorption transporters, and suppressing NF-κB/NLRP3 pathway in hyperuricemia. The results provide the evidence that FxUD may be potential for the treatment of hyperuricemia with kidney inflammation.

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Novel xanthine oxidase-based cell model using HK-2 cell for screening antihyperuricemic functional compounds

Cited by 45 publications

References 29 publications

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

Effect on purine releasement of Lentinus edodes by different food processing techniques

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

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Novel xanthine oxidase-based cell model using HK-2 cell for screening antihyperuricemic functional compounds

Cited by 45 publications

References 29 publications

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

Protective effects of tuna meat oligopeptides (TMOP) supplementation on hyperuricemia and associated renal inflammation mediated by gut microbiota

Effect on purine releasement of Lentinus edodes by different food processing techniques

Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects

Contact Info

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Novel xanthine oxidase-based cell model using HK-2 cell for screening antihyperuricemic functional compounds