NtLTP4, a lipid transfer protein that enhances salt and drought stresses tolerance in Nicotiana tabacum

Xu, Yang; Zheng, Xinxin; Song, Yunzhi; Zhu, Lifei; Yu, Zipeng; Gan, Li‐Ming; Zhou, Shumei; Liu, Hongmei; Wen, Fujiang; C, Zhu

doi:10.1038/s41598-018-27274-8

Cited by 65 publications

(43 citation statements)

References 56 publications

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“…The Casparian strips in the roots play an important role in preventing the non-selective apoplastic bypass of salts into the stele, thereby decreasing the accumulation of toxic ions under salt stress [32]. Na + accumulation was lower in tobacco lines overexpressing NtLTP4 when compared with those of wild type lines after salt treatment [33]. Our data suggest that salt-induced increase in the levels of LTP contribute to a greater deposition of suberin in the Casparian bands, bringing about increased salt tolerance.…”

Section: Discussionmentioning

confidence: 99%

Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots

et al. 2019

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Lipid transfer proteins (LTPs) are a class of small, cationic proteins that bind and transfer lipids and play an important role in plant defense. However, their precise biological role in plants under adverse conditions including salinity and possible regulation by stress hormone abscisic acid (ABA) remains unknown. In this work, we studied the localization of LTPs and ABA in the roots of pea plants using specific antibodies. Presence of LTPs was detected on the periphery of the cells mainly located in the phloem. Mild salt stress (50 mM NaCI) led to slowing plant growth and higher immunostaining for LTPs in the phloem. The deposition of suberin in Casparian bands located in the endoderma revealed with Sudan III was shown to be more intensive under salt stress and coincided with the increased LTP staining. All obtained data suggest possible functions of LTPs in pea roots. We assume that these proteins can participate in stress-induced pea root suberization or in transport of phloem lipid molecules. Salt stress increased ABA immunostaining in pea root cells but its localization was different from that of the LTPs. Thus, we failed to confirm the hypothesis regarding the direct influence of ABA on the level of LTPs in the salt-stressed root cells.

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Section: Discussionmentioning

confidence: 99%

Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots

et al. 2019

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“…Redundant dye solution was washed away with boiled ethanol, and the leaves were imaged. ImageJ software was used for quantitative analysis of H 2 O 2 and O 2 − intensity [80].…”

Section: Histochemical Staining Of Reactive Oxygen Speciesmentioning

confidence: 99%

Ultrahigh-activity immune inducer from Endophytic Fungi induces tobacco resistance to virus by SA pathway and RNA silencing

et al. 2020

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Background: Plant viruses cause severe economic losses in agricultural production. An ultrahigh activity plant immune inducer (i.e., ZhiNengCong, ZNC) was extracted from endophytic fungi, and it could promote plant growth and enhance resistance to bacteria. However, the antiviral function has not been studied. Our study aims to evaluate the antiviral molecular mechanisms of ZNC in tobacco. Results: Here, we used Potato X virus (PVX), wild-type tobacco and NahG transgenic tobacco as materials to study the resistance of ZNC to virus. ZNC exhibited a high activity in enhancing resistance to viruses and showed optimal use concentration at 100-150 ng/mL. ZNC also induced reactive oxygen species accumulation, increased salicylic acid (SA) content by upregulating the expression of phenylalanine ammonia lyase (PAL) gene and activated SA signaling pathway. We generated transcriptome profiles from ZNC-treated seedlings using RNA sequencing. The first GO term in biological process was positive regulation of post-transcriptional gene silencing, and the subsequent results showed that ZNC promoted RNA silencing. ZNC-sprayed wild-type leaves showed decreased infection areas, whereas ZNC failed to induce a protective effect against PVX in NahG leaves. Conclusion: All results indicate that ZNC is an ultrahigh-activity immune inducer, and it could enhance tobacco resistance to PVX at low concentration by positively regulating the RNA silencing via SA pathway. The antiviral mechanism of ZNC was first revealed in this study, and this study provides a new antiviral bioagent.

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“…Further analysis revealed that the SNO-proteins identified were localized to the chloroplast, cytoplasm, cell wall, or vacuole with the majority showing localization to the chloroplast and having diverse functions in photosynthetic electron transfer and carbon utilization. In addition, several, including bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi, glutathione S-transferase, mannose-binding lectin protein, chlorophyll B-binding protein, and Beta-carbonic anhydrase, have been implicated in stomatal movement in response to stress [43][44][45][46][47]. This suggests that these proteins have important roles in stomatal function that may be regulated via S-nitrosylation.…”

Section: Guard Cell S-nitroso-proteomic Changes In Response To Flg22 mentioning

confidence: 99%

S-Nitroso-Proteome Revealed in Stomatal Guard Cell Response to Flg22

Lawrence

Gaitens

Guan

et al. 2020

IJMS

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Nitric oxide (NO) plays an important role in stomata closure induced by environmental stimuli including pathogens. During pathogen challenge, nitric oxide (NO) acts as a second messenger in guard cell signaling networks to activate downstream responses leading to stomata closure. One means by which NO's action is achieved is through the posttranslational modification of cysteine residue(s) of target proteins. Although the roles of NO have been well studied in plant tissues and seedlings, far less is known about NO signaling and, more specifically, protein S-nitrosylation (SNO) in stomatal guard cells. In this study, using iodoTMTRAQ quantitative proteomics technology, we analyzed changes in protein SNO modification in guard cells of reference plant Arabidopsis thaliana in response to flg22, an elicitor-active peptide derived from bacterial flagellin. A total of 41 SNO-modified peptides corresponding to 35 proteins were identified. The proteins cover a wide range of functions, including energy metabolism, transport, stress response, photosynthesis, and cell-cell communication. This study creates the first inventory of previously unknown NO responsive proteins in guard cell immune responses and establishes a foundation for future research toward understanding the molecular mechanisms and regulatory roles of SNO in stomata immunity against bacterial pathogens.

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NtLTP4, a lipid transfer protein that enhances salt and drought stresses tolerance in Nicotiana tabacum

Cited by 65 publications

References 56 publications

Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots

Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots

Ultrahigh-activity immune inducer from Endophytic Fungi induces tobacco resistance to virus by SA pathway and RNA silencing

S-Nitroso-Proteome Revealed in Stomatal Guard Cell Response to Flg22

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