Nuclear Blebs in Human Leukaemic Cells

McDuffie, Norton G.

doi:10.1038/2141341a0

Cited by 41 publications

(11 citation statements)

References 2 publications

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“…Acute myeloid leukemia (AML) Ahearn et al 1974;Anderson 1966;Eguchi et al 1977;McDuffie 1967;Olins et al 1998;Tricot and Broeckaert-Van Orshoven 1984 Eosinophils Djaldetti et al 1993 Chronic myeloid leukemia (CML) a Thiele et al 1990 Chronic megakaryocytic-granulocytic leukemia (CMGM) Thiele et al 1977 Myelomonocytic leukemia erythrocyte precursors Maldonado et al 1976 Eosinophils Dalton et al 1987 Acute non-lymphocytic leukemia (ANLL)…”

Section: Myeloid Leukemiamentioning

confidence: 98%

Nuclear envelope-limited chromatin sheets (ELCS) and heterochromatin higher order structure

Olins

2009

Chromosoma

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The interphase nucleus and nuclear envelope can acquire a myriad of shapes in normal or pathological cell states. There exist a wide variety of indentations and invaginations, of protrusions and evaginations. It has been difficult to classify and name all of these nuclear shapes and, consequently, a barrier to understanding the biochemical and biophysical causes. This review focuses upon one type of nuclear envelope shape change, named "nuclear envelope-limited chromatin sheets" (ELCS), which appears to involve exaggerated nuclear envelope growth, carrying with it one or more layers of approximately 30 nm diameter heterochromatin. A hypothesis on the formation of ELCS is proposed, relating higher order heterochromatin structure in an interphase nucleus, nuclear envelope growth, and nuclear envelope-heterochromatin interactions.

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Section: Myeloid Leukemiamentioning

confidence: 98%

Nuclear envelope-limited chromatin sheets (ELCS) and heterochromatin higher order structure

Olins

2009

Chromosoma

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“…The few intact cells that survived its action showed nuclear pyknosis and pocketing. This latter phenomenon, which is believed to be due to an anomaly of mitosis during maturation, is often seen in leukaemic cells and in cells exposed to nitrogen mustard or ionising radiation (Ahearn et al, 1967;McDuffie, 1967, Duplan et al, 1969 and suggests that cytotoxin affects not only the cell membrane but the nucleus as well. The fact that cytotoxin labelled with colloidal gold attached to PMNL granules, nuclei and cell membrane, supports the view that the action of cytotoxin is not restricted solely to the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

The effect of Pseudomonas aeruginosa cytotoxin and toxin A on human polymorphonuclear leukocytes

Bishop

Baltch²,

Hill³

et al. 1987

Journal of Medical Microbiology

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Summary. After exposure to cytotoxin or toxin A of Pseudomonas aeruginosa, the ultrastructure of resting and phagocytosing human polymorphonuclear leukocytes (PMNL) and of cells of P . aeruginosa strain 1348A was studied by transmission (TEM) and scanning (SEM) electronmicroscopy, and by light microscopy (LM) after histochemical staining of cytoplasmic granules. Cytotoxin caused marked clumping and destruction of PMNL, pyknotic nuclear changes with bleb formation, and release of cytoplasmic granules; phagocytosis was markedly diminished. In contrast, after exposure to toxin A, PMNL phagocytosed actively, but their cytoplasmic pseudopodia were markedly irregular and their nuclei pyknotic. Colloidal-gold-labelled cytotoxin showed an affinity for the cytoplasmic membranes, nuclei and granules of PMNL. Cytotoxin had no apparent effect on cells of P . aeruginosa strain 1348A but there was polar separation of the cytoplasmic membrane in bacteria exposed to toxin A. Cytotoxin and toxin A appear to be important in the pathogenesis of infections caused by P. aeruginosa.

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“…In this connection, the prevalence of nuclear pockets and bridges should be emphasized; al though they have been observed also in normal white blood cells [6,10], they are reported to be more numerous in cases of leukemia [1][2][3][7][8].…”

Section: Discussionmentioning

confidence: 99%