Nuclear envelope-distributed CD147 interacts with and inhibits the transcriptional function of RING1 and promotes melanoma cell motility

Chen, Junchen; Peng, Cong; Li, Lei; Zhang, Jianglin; Zeng, Weiqi; Chen, Xiang

doi:10.1371/journal.pone.0183689

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…The human melanoma cell lines A375, SK-Mel-5, and SK-Mel-28 were purchased from the ATCC (Manassas, VA, USA), and the BRAFi-resistant cell line, called RA, was generated as described in a previous study [9,29]. JB6 mouse skin epidermal cell line, BJ human skin fibroblast cell line,PIG1 human melanocyte cell line, H9C2 human heart myoblast cell line were purchased from the ATCC.…”

Section: Cell Lines and Culturementioning

confidence: 99%

A novel chalcone derivative has antitumor activity in melanoma by inducing DNA damage through the upregulation of ROS products

Li¹,

Long²,

Su³

et al. 2020

Cancer Cell Int

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Background: Melanoma is one of the most aggressive tumors with the remarkable characteristic of resistance to traditional chemotherapy and radiotherapy. Although targeted therapy and immunotherapy benefit advanced melanoma patient treatment, BRAFi (BRAF inhibitor) resistance and the lower response rates or severe side effects of immunotherapy have been observed, therefore, it is necessary to develop novel inhibitors for melanoma treatment. Methods:We detected the cell proliferation of lj-1-59 in different melanoma cells by CCK 8 and colony formation assay. To further explore the mechanisms of lj-1-59 in melanoma, we performed RNA sequencing to discover the pathway of differential gene enrichment. Western blot and Q-RT-PCR were confirmed to study the function of lj-1-59 in melanoma. Results:We found that lj-1-59 inhibits melanoma cell proliferation in vitro and in vivo, induces cell cycle arrest at the G2/M phase and promotes apoptosis in melanoma cell lines. Furthermore, RNA-Seq was performed to study alterations in gene expression profiles after treatment with lj-1-59 in melanoma cells, revealing that this compound regulates various pathways, such as DNA replication, P53, apoptosis and the cell cycle. Additionally, we validated the effect of lj-1-59 on key gene expression alterations by Q-RT-PCR. Our findings showed that lj-1-59 significantly increases ROS (reactive oxygen species) products, leading to DNA toxicity in melanoma cell lines. Moreover, lj-1-59 increases ROS levels in BRAFi -resistant melanoma cells, leading to DNA damage, which caused G2/M phase arrest and apoptosis. Conclusions:Taken together, we found that lj-1-59 treatment inhibits melanoma cell growth by inducing apoptosis and DNA damage through increased ROS levels, suggesting that this compound is a potential therapeutic drug for melanoma treatment.

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Section: Cell Lines and Culturementioning

confidence: 99%

A novel chalcone derivative has antitumor activity in melanoma by inducing DNA damage through the upregulation of ROS products

Li¹,

Long²,

Su³

et al. 2020

Cancer Cell Int

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“…SKCM is the most malignant tumor among all skin tumors, owing to its highly invasive and metastatic potential[8], and the prognosis is extremely poor. Although SKCM only accounts for 4% of all skin cancers, it accounts for nearly 80% of all skin cancer-related deaths [9][10][11]. Early melanoma can be effectively treated by prompt surgical resection [12].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Hub Genes and Pathways Predicting Pathogenesis in Skin Cutaneous Melanoma

et al. 2022

Preprint

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Background: Malignant melanoma (MM) is one of the tumors with the worst prognosis among skin malignancies. However, its pathogenesis and potential therapeutic targets still need to be explored to a large extent.Methods: In the present study, we found three gene expression profiles and screened differentially expressed genes (DEGs) between melanoma and normal tissues. The DEGs were dealt with gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), protein–protein interaction (PPI), and survival analyses, while the connectivity map (CMap) database was used to predict candidate small molecules that may reverse the biological state of MMResults: we detected the expression of the most relevant core gene in vitro and checked the impact of the top predicted potential drug against the proliferation of MM cell lines. EXO1 were associated with unfavorable MM prognosis, ten hub genes were chose among the 77 up-regulated DEGs and 41 down-regulated DEGs, and three promising small molecule drugs for treating MM were identified. Simultaneously, we found that the highest predicted potential drug rifabutin significantly inhibits on the proliferation of melanoma cells and significantly reverses the expression of EXO1.In summary, the findings of the present study suggest that the ten hub genes and pathways identified may be closely related to MM pathogenesis.Conclusions: EXO1 overexpression could be a hopeful biomarker for revealing poor prognosis in patients with MM, while the rifabutin for the development of potential drugs to treat MM.

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“…Alteration of the SCN8A gene (encoding type VIII alpha subunit of voltage gated sodium channel), which was down-regulated in the sensitive-and upregulated in the resistant spheroids, was described for several tumour types and it has been found that SCN8A gene expression level is significantly lower in tumour tissues compared to paired normal tissues [47]. Similarly, ring finger protein-1 (RING1) was also found as a differently expressed gene, which is involved in epigenetic regulation in cancer [48], where it acts as a transcriptional repressor and plays an important role in the development of aggressive phenotypes in melanoma [49]. Furthermore, upregulation of the ABHD4 gene was also common in resistant spheroids compared to sensitive ones.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Signature of BRAF Inhibitor Resistant Melanoma Spheroids

Koroknai

Patel

Szász

et al. 2020

Pathol. Oncol. Res.

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In vitro cell cultures are frequently used to define the molecular background of drug resistance. The majority of currently available data have been obtained from 2D in vitro cultures, however, 3D cell culture systems (spheroids) are more likely to behave similarly to in vivo conditions. Our major aim was to compare the gene expression signature of 2D and 3D cultured BRAFV600E mutant melanoma cell lines. We successfully developed BRAF-drug resistant cell lines from paired primary/ metastatic melanoma cell lines in both 2D and 3D in vitro cultures. Using Affymetrix Human Gene 1.0 ST arrays, we determined the gene expression pattern of all cell lines. Our analysis revealed 1049 genes (562 upregulated and 487 downregulated) that were differentially expressed between drug-sensitive cells grown under different cell cultures. Pathway analysis showed that the differently expressed genes were mainly associated with the cell cycle, p53, and other cancer-related pathways. The number of upregulated genes (72 genes) was remarkably fewer when comparing the resistant adherent cells to cells that grow in 3D, and were associated with cell adhesion molecules and IGF1R signalling. Only 1% of the upregulated and 5.6% of the downregulated genes were commonly altered between the sensitive and the resistant spheroids. Interestingly, we found several genes (BNIP3, RING1 and ABHD4) with inverse expression signature between sensitive and resistant spheroids, which are involved in anoikis resistance and cell cycle regulation. In summary, our study highlights gene expression alterations that might help to understand the development of acquired resistance in melanoma cells in tumour tissue.

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Nuclear envelope-distributed CD147 interacts with and inhibits the transcriptional function of RING1 and promotes melanoma cell motility

Cited by 9 publications

References 35 publications

A novel chalcone derivative has antitumor activity in melanoma by inducing DNA damage through the upregulation of ROS products

A novel chalcone derivative has antitumor activity in melanoma by inducing DNA damage through the upregulation of ROS products

Identification of Potential Hub Genes and Pathways Predicting Pathogenesis in Skin Cutaneous Melanoma

Gene Expression Signature of BRAF Inhibitor Resistant Melanoma Spheroids

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