Nuclear envelope dynamics during mammalian spermatogenesis: new insights on male fertility

Pereira, Cátia D.; Serrano, Joana B.; Martins, Filipa; Silva, Edgar F. da Cruz e; Rebelo, Sandra

doi:10.1111/brv.12498

Cited by 40 publications

(41 citation statements)

References 100 publications

(356 reference statements)

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“…The mouse centrosomes and telomeres showed a peripheral clustering localization, whereas a nuclear interior localization is observed in the case of human telomeres (Weierich et al, 2003). An exception was observed during meiosis, when the LINC complex proteins participate in bouquet formation and in the sequestration of telomeres into a subnuclear area together with the paring of homologous chromosomes (Ding et al, 2007; Lottersberger, Karssemeijer, Dimitrova, & Lange, 2015; Penkner et al, 2009; Pereira, Serrano, Martins, da Cruz e Silva, & Rebelo, 2019).…”

Section: Telomere Maintenancementioning

confidence: 99%

Nuclear envelope dysfunction and its contribution to the aging process

et al. 2020

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The nuclear envelope (NE) is the central organizing unit of the eukaryotic cell serving as a genome protective barrier and mechanotransduction interface between the cytoplasm and the nucleus. The NE is mainly composed of a nuclear lamina and a double membrane connected at specific points where the nuclear pore complexes (NPCs) form. Physiological aging might be generically defined as a functional decline across lifespan observed from the cellular to organismal level. Therefore, during aging and premature aging, several cellular alterations occur, including nuclear‐specific changes, particularly, altered nuclear transport, increased genomic instability induced by DNA damage, and telomere attrition. Here, we highlight and discuss proteins associated with nuclear transport dysfunction induced by aging, particularly nucleoporins, nuclear transport factors, and lamins. Moreover, changes in the structure of chromatin and consequent heterochromatin rearrangement upon aging are discussed. These alterations correlate with NE dysfunction, particularly lamins’ alterations. Finally, telomere attrition is addressed and correlated with altered levels of nuclear lamins and nuclear lamina‐associated proteins. Overall, the identification of molecular mechanisms underlying NE dysfunction, including upstream and downstream events, which have yet to be unraveled, will be determinant not only to our understanding of several pathologies, but as here discussed, in the aging process.

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Section: Telomere Maintenancementioning

confidence: 99%

Nuclear envelope dysfunction and its contribution to the aging process

et al. 2020

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“…One of the most remarkable cellular changes accompanying sperm differentiation is the remodeling of the nuclear architecture [42]. In the mouse and rat, in the early steps of spermiogenesis, round spermatids have a spherical nucleus located in the center of the cell and the chromatin is decondensed (steps 1 to 7).…”

Section: Nuclear Elongationmentioning

confidence: 99%

Sperm Differentiation: The Role of Trafficking of Proteins

Teves

Roldán

Krapf

et al. 2020

IJMS

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Sperm differentiation encompasses a complex sequence of morphological changes that takes place in the seminiferous epithelium. In this process, haploid round spermatids undergo substantial structural and functional alterations, resulting in highly polarized sperm. Hallmark changes during the differentiation process include the formation of new organelles, chromatin condensation and nuclear shaping, elimination of residual cytoplasm, and assembly of the sperm flagella. To achieve these transformations, spermatids have unique mechanisms for protein trafficking that operate in a coordinated fashion. Microtubules and filaments of actin are the main tracks used to facilitate the transport mechanisms, assisted by motor and non-motor proteins, for delivery of vesicular and non-vesicular cargos to specific sites. This review integrates recent findings regarding the role of protein trafficking in sperm differentiation. Although a complete characterization of the interactome of proteins involved in these temporal and spatial processes is not yet known, we propose a model based on the current literature as a framework for future investigations.

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“…Spermatogenesis is a complicated process which includes the proliferation and differentiation of spermatogonia, meiosis, and spermatogenesis [30]. Spermatozoa are produced in the seminiferous tubules of mouse testes and transported to the epididymides for maturation.…”

Section: Effects On the Quality Of Spermmentioning

confidence: 99%

Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

Lin

Chen

et al. 2020

Preprint

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Background: Increased applications of quantum dots (QDs) in biomedical field have aroused attention for their potential toxicological effects. Although many studies have been carried out on the toxicity of QDs, their effects on reproductive and development are still unclear. In this study, we systematically evaluated the male reproductive toxicity and developmental toxicity of CdSe/ZnS QDs in BALB/c mice.Results: The male mice were injected intravenously with CdSe/ZnS QDs at the dosage of 2.5 mg/kg BW or 25 mg/kg BW, respectively, and the survival status, biodistribution of QDs in testes, serum sex hormone levels, histopathology, sperm motility and acrosome integrity was measured on Day 1, 7, 14, 28 and 42 after injection. On Day 35 after treatment, male mice were housed with non-exposed female mice, and then offspring number, body weight, organ index and histopathology of major organs, blood routine and biochemical tests of offspring were measured to evaluate the fertility and offspring health. The results showed that CdSe/ZnS QDs could rapidly distribute in the testis, and the fluorescence of QDs could still be detected on Day 42 post-injection. QDs had no adverse effect on the structure of testis and epididymis, but high-dose QDs could induce apoptosis of Leydig cells in testis at an early stage. No significant differences in survival of state, body weight organ index of testis and epididymis, sex hormones levels, sperm quality, sperm acrosome integrity and fertility of male mice were observed in QDs exposed groups. However, the development of offspring was obviously influenced, which was mainly manifested in the slow growth of offspring, changes of organ index of main organs, and the abnormality of liver and kidney function parameters.Conclusion: Our findings revealed that CdSe/ZnS QDs were able to cross the blood-testis barrier (BTB), produce no discernible toxic effects on the male reproductive system, but could affect the healthy growth of future generations to some extent. In view of the broad application prospect of QDs in biomedical fields, our findings might provide insight into the biological safety evaluation of the reproductive health of QDs.

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Nuclear envelope dynamics during mammalian spermatogenesis: new insights on male fertility

Cited by 40 publications

References 100 publications

Nuclear envelope dysfunction and its contribution to the aging process

Nuclear envelope dysfunction and its contribution to the aging process

Sperm Differentiation: The Role of Trafficking of Proteins

Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

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