Nuclear maturation kinetics and in vitro embryo development of cattle oocytes prematured with butyrolactone I combined or not combined with roscovitine

Adona, Paulo Roberto; Pires, Pedro Ratto Lisboa; Quetglas, Maria Daniela; Schwarz, Kátia Regina Lancellotti; Leal, Cláudia Lima Verde

doi:10.1016/j.anireprosci.2007.06.013

Cited by 33 publications

(40 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In bovine, the embryo culture conditions are a well documented process (Parrish et al, 1994;Holm et al, 1999;Papis et al, 2007;Sargirkaya et al, 2007;Adona et al, 2008;Corrêa et al, 2008). Oocyte in-vitro maturation is a viable phenomenon as oocyte matured, fertilized and cultured in vitro can generate embryos with full developmental potential after embryo transfer.…”

Section: Resultsmentioning

confidence: 99%

“…Because maturation media of in vitro bovine embryo systems are generally composed by TCM-199 medium supplemented with of 0.5 μg mL -1 FSH, 5 μg mL -1 LH and 10% FCS (Sargirkaya et al, 2007;Adona et al, 2008;Corrêa et al, 2008) the maturation medium of control group was supplemented with gonadotropins.…”

Section: Resultsmentioning

confidence: 99%

“…The in vitro culture procedures were based on methods described previously (Adona et al, 2008;Corrêa et al, 2008). Ovaries obtained from slaughterhouse cows were transported to the laboratory in saline solution (NaCl 0.9%) supplemented with 100 UI mL -1 penicillin G and 100 μg mL -1 streptomycin sulfate at 35°C within 2 h. Cumulus-oocyte complexes (COCs) were aspirated from 2-to 6-mm follicles with an 18 gauge needle and a 10 mL syringe and the aspirated follicular fluid was pooled in a 15 mL conical tube.…”

Section: In Vitro Maturation Fertilization and Embryo Culture Procedmentioning

confidence: 99%

“…It has been reported that FSH and LH added to maturation medium have a beneficial effect on the competence of oocytes to develop in vitro to the blastocyst stage (Papis et al, 2007;Adona et al, 2008;Corrêa et al, 2008). Although producing controversial results (Holm et al, 2002), FCS is successfully used as protein supplement in oocyte maturation medium (Sargirkaya et al, 2007;Adona et al, 2008;Corrêa et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The basic maturation media used in bovine embryo culture systems are generally composed of TCM-199 medium supplemented with a combination of FSH, LH and fetal calf serum (FCS) (Sargirkaya et al, 2007;Adona et al, 2008;Corrêa et al, 2008). It has been reported that FSH and LH added to maturation medium have a beneficial effect on the competence of oocytes to develop in vitro to the blastocyst stage (Papis et al, 2007;Adona et al, 2008;Corrêa et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum -FCS) at 39°C and 5% CO 2 in air. After 24 hours of culture in MM with 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH; 10 -9 M MEL) or 10 -9 M MEL, 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10 -9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM. Key words: comet assay, embryos, gonadotropins, in vitro fertilization, melatonin Melatonina no meio de maturação não melhorou as taxas de maturação dos ovócitos, de desenvolvimento embrionário e a fragmentação do DNA dos embriões bovinos RESUMO: Melatonin (MEL) atua como um potente redutor de radicais livres. Efeito direto da MEL na função gonadal também foi observado. Existem poucos estudos relacionados ao efeito da MEL durante a maturação no desenvolvimento embrionário in vitro. Avaliou-se a adição de MEL no meio de maturação (sem gonadotrofinas) nas taxas de maturação nuclear e de desenvolvimento embrionário e na incidência de fragmentação do DNA nos embriões produzidos in vitro. Complexos cumulus-ovócitos foram aspirados de ovários provenientes de matadouros e cultivados em meio de maturação (Meio TCM-199 suplementado com 10% de soro fetal bovino) a 39°C e 5% CO 2 em ar. Após 24 horas de cultivo em meio de maturação com 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH; 10 -9 M de MEL ou 10 -9 M de MEL, 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH, os ovócitos foram corados com Hoechst 33342 para avaliação da taxa de maturação nuclear. A fragmentação do DNA dos blastocistos foi avaliada pela técnica do Cometa. Não houve efeito da MEL adicionada ao meio de maturação, com ou sem gonadotrofinas, nas taxas de maturação nuclear, clivagem e blastocistos. Os percentuais variaram entre 88% a 90%, 85% a 88% e 42% a 46%, respectivamente. O grau de fragmentação do DNA também não foi afetado pela adição de MEL ao meio de matur...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: In Vitro Maturation Fertilization and Embryo Culture Procedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

show abstract

Effect of C-type natriuretic peptide pretreatment on in vitro bovine oocyte maturation

Zhang

Fan

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

C-type natriuretic peptide (CNP) has been considered as a physiological meiotic inhibitor that stimulates the cGMP production by cumulus cell natriuretic peptide receptor 2 (NPR2), which inhibits oocyte phosphodiesterase type 3 activity and increases cAMP. In this study, we explored the effect of CNP pretreatment on the in vitro maturation (IVM) of bovine oocytes by examining changes in cleavage rate, blastocyst formation, mitochondrial DNA (mtDNA) copy number, reactive oxygen species (ROS) level, glutathione (GSH) content, and redox state. Our results showed that 200 nM CNP could effectively maintain meiotic arrest of bovine oocytes in vitro within 6 h. The two-step IVM system in which oocytes were pretreated with 200 nM CNP for 6 h and then cultured IVM for 28 h yielded a significantly (P < 0.05) increased blastocyst rate and cell number after in vitro fertilization (IVF) while compared to the conventional one-step IVM method. In addition, in comparison with the conventional 24-h matured oocyte, oocytes pretreated with 200 nM CNP for 6 h followed by 28 h IVM resulted in significantly (P < 0.05) higher mtDNA copy number and ROS levels in oocytes, while GSH level significantly (P < 0.05) decreased. Remarkably, regardless of treatment, no changes were observed in FAD++, NAD(P)H autofluorescence intensity, and redox ratio (FAD++/NAD(P)H) within the oocytes, maintaining a healthy metabolic equilibrium of redox throughout the two-step IVM. In conclusion, these results indicate that CNP pretreatment could dramatically improve the quality of bovine oocytes during in vitro maturation.

show abstract

Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals

Leal

Monteiro

Souza-Fabjan

et al. 2018

Animal Reproduction Science

View full text Add to dashboard Cite

Nuclear maturation kinetics and in vitro embryo development of cattle oocytes prematured with butyrolactone I combined or not combined with roscovitine

Cited by 33 publications

References 19 publications

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Effect of C-type natriuretic peptide pretreatment on in vitro bovine oocyte maturation

Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals

Contact Info

Product

Resources

About