Nuclear Morphometry in African Breast Cancer

Abstract

Three hundred cases of invasive breast cancer diagnosed between 1983 and 1999 in Calabar, Nigeria were analysed to determine the nuclear morphometric variables, and evaluate the prognostic potential of nuclear morphometry in Nigerian breast cancers. The necessary follow-up was available for 129 patients. The nuclear area was the most valuable variable. In the Nigerian material, the mean nuclear area (MNA) (SD) was 89.2 (34.0) µm 2 . MNA was significantly higher in tumours of the postmenopausal than premenopausal (p = 0.0405), in LN+ than LN-(p = 0.0202) patients, and in tumours over 3 cm than smaller ones (p < 0.0001). There were also significant differences between different clinical stages, histological grades, and histological types of tumours. Significant correlations were observed between MNA and histological grade (r = 0.64), standard mitotic index (r = 0.45) and tumour size (r = 0.20). MNA as a continuous variable was a statistically significant prognosticator in the whole material (p = 0.0281), and among the postmenopausal patients (p = 0.0238). Univariate cox´s regression demonstrated one significant grading cutpoint at MNA = 111 µm 2 , which divided the material into two groups of different survival. The development of a morphometric grading system optimal for the Nigerian material could use the latter cut-point between nuclear scores 2 and 3 in the grading system. The earlier proven cut-point of 47 µm 2 could be used between nuclear scores 1 and 2.

Role of nuclear morphometry in breast cancer and its correlation with cytomorphological grading of breast cancer: A study of 64 cases

“…Many morphometric studies have been done on breast histology and their results can be compared with our findings on breast cytology. [ 8 9 10 11 12 13 14 15 16 17 ]…”

“…Similarly, Kalhan et al . [ 18 ] in their study on cytological aspirates and Ikpatt et al .,[ 14 ] Al-Obaidi et al .,[ 17 ] and Radwan et al . [ 15 ] in their study on histopathological sections of breast found a significant association between nuclear size parameters and grades of carcinoma.…”

The Quantitative ER Immunohistochemical Analysis in Breast Cancer: Detecting the 3 + 0, 4 + 0, and 5 + 0 Allred Score Cases

“…Finally, it represents a great benefit for patients with BC, since receptor expression analysis is crucial for the determination of the prognostic indexes [25]. Although no generally accepted standards for morphometric and photometric analysis are available [37], one can say that the results of this study can be useful for comparison between different histological variants of BC. The Allred scoring system is based on the semi-quantitative estimation of percentage abundance of positive cancer cell nuclei and staining intensity, where the expression limit is 10% of weakly or 1% of medium-stained cancer cell nuclei [18].…”

Nuclear morphometry of mammary tumors of dogs and histopathological diagnosis

“…З гістологічною класифікацією корелює цитологічна система класифікації і цитологічні дослідження є рекомендовані для прогнозу (No authors, 1997). В літературі найбільше предствлені результати досліджень у людини (Pienta et al, 1991;Ruiz et al, 1999;Ikpatt et al, 2002;Radwan et al, 2003;Kalhan et al, 2010;Chowdhury et al, 2018;Dolka et al, 2018). Є повідомлення що цитологічний метод, який розроблений і прийнятий для людини, може бути адаптований для ветеринарної цитології (Dolka et al, 2018), проте результатів отриманих у собак є небагато і тому необхідними є додаткові дослідження.…”