Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts

Krämer, Susanne

doi:10.1017/s0031182021000068

Cited by 15 publications

(15 citation statements)

References 313 publications

(530 reference statements)

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“…13 Comparision between S. cerevisae and T. cruzi components of mRNA export coupled to transcription/splicing. The figure contains the major components of S. cerevisiae as described on Kohler and Hurt (2007) ( 130 ) and Kramer (2021) ( 47 ). To simplify, components of Peripheral Nuclear Granules and Nuclear Pore Complex are omitted.…”

Section: Discussionmentioning

confidence: 99%

“…Although many components of the RNA export machinery are known from animals, fungi, plants, and other organisms, in silico identification of components in trypanosomatid genomes is unreliable due to high divergence and few proteins appear conserved or recognizable ( 46 ). In fact, several nuclear complexes and nuclear-pore-associated proteins that play major roles in mRNA export in opisthokonts are lacking in trypanosomes, and a recent comparative review described the main differences in nuclear mRNA maturation and mRNA export ( 47 ). While UAP56/Sub2 ( 48 ) and TAP/Mex67 ( 49 , 50 , 51 ) are essential for mRNA transport in trypanosomes, little else has been identified with significant roles ( 49 , 51 , 52 , 53 ).…”

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confidence: 99%

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Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes

Inoue

Domingues

Serpeloni

et al. 2022

Molecular & Cellular Proteomics

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes

Inoue

Domingues

Serpeloni

et al. 2022

Molecular & Cellular Proteomics

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“…The only other DExD/H RNA helicase that has been identified in the context of rRNA maturation in trypanosomes is Mtr4 30 . Mtr4 localises to the nucleoplasm 39 and is likely to be part of the TRAMP complex and thus involved in general RNA surveillance in the nucleus together with the nuclear exosome 30,48 . Depletion of either Mtr4 or exosomal subunits cause accumulation of ribosomal precursor RNAs 30,31 .…”

Section: Discussionmentioning

confidence: 99%

The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei

Bakari-Soale

Ikenge

Scheibe

et al. 2021

Preprint

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The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process.

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“…One explores the relative conservation of mRNA biogenesis, processing and export between trypanosomes and animals and fungi. This provides a comprehensive framework of great value to anyone teaching or embarking upon the field of protozoan gene regulation, as well as those with specific interests in protist evolution (Kramer, 2021). A complementary minireview by Paris on tRNA export examines conservation of a more discrete but equally vital process (Paris, 2021).…”

Section: Gene Expression and Nuclear Organizationmentioning

confidence: 99%

Kinetoplastid cell biology and genetics, from the 2020 British Society for Parasitology Trypanosomiasis and Leishmaniasis symposium, Granada, Spain

et al. 2021

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The British Society for Parasitology (BSP) holds a biannual symposium devoted to the Kinetoplastids, and seeks to cover the full gamut of research into these important organisms, and alternates with the Woods Hole KMCB meeting that serves a similar community. While normally embedded within the main BSP Spring meeting, on several occasions the symposium has enjoyed the opportunity of being hosted on mainland Europe. In 2020 the BSP was fortunate to spend some time in Granada in Spain, where a superb meeting with excellent science in a spectacular setting was overshadowed by news of an emerging novel coronavirus. In this editorial we hope to have captured some of that excellent science and to highlight aspects of the many great papers and reviews in this special issue, as well as provide a few images from the meeting, which we hope for this who attended will bring back some fond memories.

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Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts

Abstract: Abstract

Cited by 15 publications

References 313 publications

Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes

Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes

The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei

Kinetoplastid cell biology and genetics, from the 2020 British Society for Parasitology Trypanosomiasis and Leishmaniasis symposium, Granada, Spain

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