Nuclear Overhauser Enhancement-Mediated Magnetization Transfer Imaging in Glioma with Different Progression at 7 T

Tang, Xiangyong; Dai, Zhuozhi; Xiao, Gang; Yan, Gen; Shen, Zhiwei; Zhang, Tao; Zhang, Guishan; Zhuang, Zerui; Shen, Yuanyu; Zhang, Zhiyan; Hu, Wei; Wu, Renhua

doi:10.1021/acschemneuro.6b00173

Cited by 10 publications

(12 citation statements)

References 38 publications

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“…This is consistent with a previous study showing that amide proton exchange is base-catalyzed [41]. The observed CEST spectrum depends on the imaging parameters as well as on the underlying tissue microenvironment [35, 39, 40]. Therefore, we optimized the CEST parameters, including pulse duration and saturation power of the irradiation RF pulse on a phantom.…”

Section: Resultssupporting

confidence: 88%

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Novel nanomedicine with a chemical-exchange saturation transfer effect for breast cancer treatment in vivo

et al. 2019

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BackgroundNanomedicine is a promising new approach to cancer treatment that avoids the disadvantages of traditional chemotherapy and improves therapeutic indices. However, the lack of a real-time visualization imaging technology to monitor drug distribution greatly limits its clinical application. Image-tracked drug delivery is of great clinical interest; it is useful for identifying those patients for whom the therapy is more likely to be beneficial. This paper discusses a novel nanomedicine that displays features of nanoparticles and facilitates functional magnetic resonance imaging but is challenging to prepare.ResultsTo achieve this goal, we synthesized an acylamino-containing amphiphilic block copolymer (polyethylene glycol-polyacrylamide-polyacetonitrile, PEG-b-P(AM-co-AN)) by reversible addition-fragmentation chain transfer (RAFT) polymerization. The PEG-b-P(AM-co-AN) has chemical exchange saturation transfer (CEST) effects, which enable the use of CEST imaging for monitoring nanocarrier accumulation and providing molecular information of pathological tissues. Based on PEG-b-P(AM-co-AN), a new nanomedicine PEG-PAM-PAN@DOX was constructed by nano-precipitation. The self-assembling nature of PEG-PAM-PAN@DOX made the synthesis effective, straightforward, and biocompatible. In vitro studies demonstrate decreased cytotoxicity of PEG-PAM-PAN@DOX compared to free doxorubicin (half-maximal inhibitory concentration (IC50), mean ~ 0.62 μg/mL vs. ~ 5 μg/mL), and the nanomedicine more efficiently entered the cytoplasm and nucleus of cancer cells to kill them. Further, in vivo animal experiments showed that the nanomedicine developed was not only effective against breast cancer, but also displayed an excellent sensitive CEST effect for monitoring drug accumulation (at about 0.5 ppm) in tumor areas. The CEST signal of post-injection 2 h was significantly higher than that of pre-injection (2.17 ± 0.88% vs. 0. 09 ± 0.75%, p < 0.01).ConclusionsThe nanomedicine with CEST imaging reflects the characterization of tumors and therapeutic functions has great potential medical applications.

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Section: Resultssupporting

confidence: 88%

“…All CEST image processing and data analysis were performed using custom-written scripts in MATLAB (Mathworks, Natick, MA, USA, R2011b). The Water Saturation Shift Reference (WASSR) method was used to correct for B 0 field inhomogeneity [40]. Regions of interest were drawn manually based on the T2-weighted images covering the entire tumor.…”

Section: Methodsmentioning

confidence: 99%

Novel nanomedicine with a chemical-exchange saturation transfer effect for breast cancer treatment in vivo

et al. 2019

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“…All rats were performed MR imaging at 28 days after the intracerebroventricular injection with Aβ25-35/sterile normal saline in the AD group/control group. Before MR examination, all rats were allowed to fast for 24 h. The rats were firstly anesthetized in an induction box with 4% isoflurane in oxygen and continued to inhale the gas mixture of 1.5%/98.5% isoflurane/oxygen via a nose tube during the MRI scanning (Tang et al, 2017). The respiration rate was monitored and maintained by animal physiological monitoring and gating systems (model 1030, SAII, United States).…”

Section: Mri Acquisition In Phantom and Animal Examinationmentioning

confidence: 99%

Reduced Cerebral Glucose Uptake in an Alzheimer’s Rat Model With Glucose-Weighted Chemical Exchange Saturation Transfer Imaging

Chen

Shen

Wang

et al. 2021

Front. Aging Neurosci.

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A correlation between the abnormal cerebral glucose metabolism and the progression of Alzheimer’s disease (AD) has been found in previous studies, suggesting that glucose alterations may be used to predict the histopathological diagnosis in AD. In this study, we investigated the dynamic changes of cerebral glucose uptake in vivo using MR glucose chemical exchange saturation transfer (glucoCEST) imaging in a rat model of AD with an intracerebroventricular (i.c.v) injection of amyloid Aβ-protein (25–35), confirmed by Morris water maze and Nissl staining. In total, 6 rats in the AD group and 6 rats in the control group that were given an injection of sterile normal saline were included. At 28 days after injection, all rats performed a 7.0 T MR exanimation, including glucoCEST, diffusion tensor imaging (DTI) and hippocampus magnetic resonance spectra (MRS), to detect the possible metabolic and structural changes in the rat brain. A significantly elevated brain glucoCEST signal in the brain of AD rats was observed, and a decreased brain glucose uptake was also explored during the progression of glucose infusion compared with those in rats of the control group. In addition, there is a significant positive correlation between glucoCEST enhancement (GCE) and myo-Inosito (Ins) in the AD group and the control group (P < 0.05). A significantly reduced number of neurons in the cortex and hippocampus in AD rats combined with the significantly longer escape and a decreased number of crossings were verified at 28 days after Aβ25–35 injection by Nissl staining and Morris water maze, respectively. Our results indicated that an abnormal brain glucose mechanism in AD rats could be detected by glucoCEST imaging, suggesting a new method to explore the occurrence and progress of diabetes-related AD or dementia.

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“…B0 map, B1 map, T1 map, and T2 map were obtained from the same slice, as described in our previous studies ( 27 – 29 ). Specifically, the B0 map was obtained by dual echo method with delta TE of 2 ms. B1 map was obtained using the double-angle method with two flip angles of 60 deg and 120 deg.…”

Section: Methodsmentioning

confidence: 99%

Mapping the Alterations of Glutamate Using Glu-Weighted CEST MRI in a Rat Model of Fatigue

Dai

et al. 2020

Front. Neurol.

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Objective: Glutamate dysregulation may play an important role in the pathophysiology of fatigue. Glutamate weighted chemical exchange saturation transfer (Glu-weighted CEST) MRI is a recently developed technology which enables measuring glutamate in vivo with high sensitivity and spatial resolution. The purpose of this study is to map the alternations of brain glutamate in a rat model of fatigue. Methods: Rats were subjected to 10 days fatigue loading procedure (fatigue group) or reared without any fatigue loading (control group). Spontaneous activities of rats in the fatigue group were recorded from 3 days before fatigue loading to 4 days after the end of fatigue loading. Glu-weighted CEST were performed following 10-day fatigue loading. Results: Rats in the fatigue group exhibited significant reduced spontaneous activities after 10-day fatigue loading. The glutamate level in the whole brain increased significantly in the fatigue group compared to that in the control group. Further analysis of glutamate in the sub-regions of brain including the prefrontal cortex, hippocampus, and striatum revealed a trend of increment, although statistical significance was not reached. Significance: The increase of glutamate level in the brain may be a crucial process involved in the pathophysiology of fatigue.

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Nuclear Overhauser Enhancement-Mediated Magnetization Transfer Imaging in Glioma with Different Progression at 7 T

Cited by 10 publications

References 38 publications

Novel nanomedicine with a chemical-exchange saturation transfer effect for breast cancer treatment in vivo

Novel nanomedicine with a chemical-exchange saturation transfer effect for breast cancer treatment in vivo

Reduced Cerebral Glucose Uptake in an Alzheimer’s Rat Model With Glucose-Weighted Chemical Exchange Saturation Transfer Imaging

Mapping the Alterations of Glutamate Using Glu-Weighted CEST MRI in a Rat Model of Fatigue

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