Nuclear polyhedrosis virus production in Heliothis armigera infected at different larval ages

Teakle, R.E.; Byrne, Valerie

doi:10.1016/0022-2011(89)90069-4

Cited by 16 publications

(7 citation statements)

References 7 publications

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“…It was defined as ''one larval equivalent'' when the virus produced at least 6 9 10 9 OBs/larva in late instars [11]. The virus yield increase exponentially with certain age of larva [29]. Generally earlier instars are highly susceptible to the virus with 100% mortality with shorter LT 50 (lethal time required to cause 50% mortality) values [18,22].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Baculoviruses from Three Major Lepidopteran Pests in the Semi-Arid Tropics of India

Kumar

Rao

Sireesha³

et al. 2011

Indian J. Virol.

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Baculoviruses were isolated from three major lepidopteran pests, Helicoverpa armigera, Spodoptera litura and Amsacta albistriga in the semi-arid tropics during natural epizootic conditions at ICRISAT fields, Patancheru, Andhra Pradesh, India. Biological, morphological and biochemical analysis identified these isolates as Nucleopolyhedroviruses (NPVs). Scanning electron microscopy of the occlusion bodies (OBs) purified from diseased larvae revealed polyhedral particles of size approximately 0.5-2.5 μm [Helicoverpa armigera Nucleopolyhedrovirus (HearNPV)], 0.9-2.92 μm [Spodoptera litura Nucleopolyhedrovirus (SpltNPV)] and 1.0-2.0 μm [Amsacta albistriga Nucleopolyhedrovirus (AmalNPV)] in diameter. Transmission electron microscopy of thin sections of OBs of the three isolates revealed up to 5-8 multiple bacilliform shaped particles packaged within a single viral envelope. The dimensions of these particles were 277.7 × 41.6 nm for HearNPV, 285.7 × 34.2 nm for SpltNPV and 228.5 × 22.8 nm for AmalNPV. Each of HearNPV and AmalNPV contained up to 6 nucleocapsids and SpltNPV contained up to 7 nucleocapsids per envelope. The estimated molecular weights of the purified OB (polyhedrin) protein of the three NPVs were 31.29-31.67 kDa. Virus yield (OBs/larva) was 5.18 ± 0.45 × 10(9) for HearNPV, 5.73 ± 0.17 × 10(9) for SpltNPV and 7.90 ± 0.54 × 10(9) for AmalNPV. The LC50 values of various NPVs against 2nd and 3rd instar larvae indicated 2.30 × 10(4) and 1.5 × 10(5) OBs/ml for HearNPV, 3.5 × 10(4) and 2.4 × 10(5) OBs/ml for SpltNPV and 5.6 × 10(4) and 3.96 × 10(5) OBs/ml for AmalNPV. The lethal time required to cause 50% mortality (LT50) for these three species were also defined. This study has shown that the NPVs infecting three major lepidopteran pests in India are multiple NPVs, and they have good potential to use as biocontrol agents against these important pests.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Baculoviruses from Three Major Lepidopteran Pests in the Semi-Arid Tropics of India

Kumar

Rao

Sireesha³

et al. 2011

Indian J. Virol.

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“…1987) and Oxyopes salticus (Hentz.) The enormous amounts of infective virus released from dead cadavers would explain how epizootics can develop in dense field from small foci of virus contamination as suggested by Teakle and Byrne (1989) in sorghum fields. on soybean.…”

Section: Discussionmentioning

confidence: 99%

Cannibalism on nuclear polyhedrosis virus infected larvae by Heliothis armigera (Hubn.) and its effect on viral infection

Dhandapani

Jayaraj

Rabindra

1993

Int. J. Trop. Insect Sci.

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Studies on cannibalism by Heliothis armigera (Hubn.) larvae on nuclear polyhedrosis virus (NPV)-infected larvae and feeding on virosed cadavers indicated that feeding was significantly higher on 4-day-old virosed cadavers followed by 1-day-old cadavers than on healthy or NPVinfected living larvae. The data revealed that cannibalism was significantly higher on NPV-infected (3 days post inoculation) larvae than either healthy larvae or those which had been just inoculated. However, the extent of feeding was higher on healthy larvae (cannibalistic feeding) than on cadavers. Significantly more number of larvae became infected by NPV when they fed on NPVkilled cadavers than on NPV-infected living larvae. Some of the larvae which had fed on either virosed cadavers or NPV-infected larvae survived. But, the disease was expressed in the pupal stage. Pupal mortality was significantly higher in larvae which fed on NPV-inoculated larvae (3 days post inoculation). The multiple choice test to find out the feeding preference of the larvae showed that the larvae had a greater preference to feed on virosed cadavers than either the healthy larvae or the host plant. The role of cannibalistic behaviour in the dissemination of the virus disease under field conditions is discussed.

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“…The isolates CBE I and NEG netted the higher C/B ratio of 1:2.32 and 1:2.48 on cotton and 1:1.36 and 1:1.48 on chickpea, respectively, than endosulfan which Gopali and Lingappa (2001) indicated the superiority of Gulbarga isolate over CBE at Dharwad District of Karnataka. It has been noted by several authors that the stabilization of virus occurs upon continuous serial passage (Teakle & Byrne 1989). The virulence of CBE I is believed to have reached the maximum as the isolates CBE I were put to test and serially passed over several decades since the work of Narayanan (1980).…”

Section: Discussionmentioning

confidence: 99%

Field efficacy of Helicoverpa armigera nucleopolyhedro-virus isolates against H. armigera (Hübner) (Lepidoptera: Noctuidae) on cotton and chickpea in Tamil Nadu

Jeyarani¹,

Sathiah²,

Karuppuchamy³

2010

Plant Prot. Sci.

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Jeyarani S., Sathiah N., Karuppuchamy P. (2010): Field efficacy of Helicoverpa armigera nucleopolyhedrovirus isolates against H. armigera (Hübner) (Lepidoptera: Noctuidae) on cotton and chickpea in Tamil Nadu. Plant Protect. Sci., 46: 116-122.Field efficacy of seven geographical isolates of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) along with an insecticide control was evaluated against H. armigera on cotton and chickpea in the Coimbatore district of Tamil Nadu, India. Among the HaNPV isolates, CBE I (Coimbatore) and NEG (Negamum) applied at 3.0 × 10 12 POB/ha to cotton and 1.5 × 10 12 POB/ha to chickpea with an adjuvant, crude sugar, significantly reduced the H. armigera larval population and increased the yield. CBE I and NEG recorded the highest yield of 2038 kg/ha and 2033 kg/ha, which was on a par with endosulfan (2026.7 kg/ha) with cost/benefit ratios of 1:2.32, 1:2.48, and 1:1.12, respectively, on cotton. In chickpea grain yields of 980, 983, and 973.3 kg/ha and cost/benefit ratios of 1:1.36, 1:1.48 and 1:0.87, respectively, in CBE I, NEG and endosulfan treated plots were obtained. The isolate RAJ (Rajasthan) recorded the lowest yield comparable to that of the untreated control in both crops.

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Nuclear polyhedrosis virus production in Heliothis armigera infected at different larval ages

Cited by 16 publications

References 7 publications

Isolation and Characterization of Baculoviruses from Three Major Lepidopteran Pests in the Semi-Arid Tropics of India

Isolation and Characterization of Baculoviruses from Three Major Lepidopteran Pests in the Semi-Arid Tropics of India

Cannibalism on nuclear polyhedrosis virus infected larvae by Heliothis armigera (Hubn.) and its effect on viral infection

Field efficacy of Helicoverpa armigera nucleopolyhedro-virus isolates against H. armigera (Hübner) (Lepidoptera: Noctuidae) on cotton and chickpea in Tamil Nadu

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