Nuclei may anchor at specific locations during nuclear determination in Paramecium caudatum

Yang, Xiaoling; Takahashi, Mihoko

doi:10.1078/0932-4739-00863

Cited by 2 publications

(4 citation statements)

References 17 publications

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“…They appeared during the third prezygotic division and were distributed around the surviving nuclei including meiotic products in the PC (Figure 2) and two prospective StPs and MiPs (Figure 3). However, such cMTs appeared neither around the extradegenerating meiotic products ( Figure 2C", 3A", B") nor the nuclear products of the first prezygotic division ( Figure 1C", 3C") and the three postzygotic divisions (Yang & Takahashi, 2002). These results might indicate cMT involvement in the surviving mechanism of the post-meiotic nuclei.…”

Section: Discussionmentioning

confidence: 89%

“…In fact, intranuclear microtubules (nMTs) exist in all normal functional micronuclei including vegetative micronuclei, two meiotic division products, the selected meiotic products in the PC, two types of pronuclei, the products of three postzygotic divisions (Gao et al, 2011b(Gao et al, , 2011cIshida et al, 1999;Nakajima et al, 2001Nakajima et al, , 2002Yang & Takahashi, 2002), and the presumptive micronuclei in exconjugants (Ishida et al, 1999;Taka et al, 2006). But such nMTs are never kept in the degenerating meiotic products or in the differentiated macronuclear anlagen (Ishida et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…The first involves a role during reciprocal pronuclear exchange in P. caudatum (Nakajima et al, 2001), which is supported by different experimental techniques in Tetrahymena (Orias et al, 1983) and other Paramecium species (Jurand, 1976;. The second is guiding the nuclei to the destined locations including the PC entrance at the completion of meiosis and anterior and posterior localization at the telophase of the third postzygotic division (Gao et al, 2011a(Gao et al, , 2011b(Gao et al, , 2011cYang & Takahashi, 2002). …”

Section: Microtubular Behavior Around the Telophase Of The Third Prezmentioning

confidence: 97%

“…Immunofluorescence staining with monoclonal antibodies of anti-α tubulin followed previous studies (Gao et al, 2011b;Yang & Takahashi, 2002). 1) Cells were fixed in 2% paraformaldehyde diluted in 2 mmol/L phosphate buffer (pH 7.0) containing 25 mmol/L KCl (PBS).…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

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Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of <I>Paramecium</I>

Wang¹,

Yuan²,

Gao³

et al. 2013

Zoological Research

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There are six micronuclear divisions during conjugation of Paramecium caudatum: three prezygotic and three postzygotic divisions. Four haploid nuclei are formed during the first two meiotic prezygotic divisions. Usually only one meiotic product is located in the paroral cone (PC) region at the completion of meiosis, which survives and divides mitotically to complete the third prezygotic division to yield a stationary and a migratory pronucleus. The remaining three located outside of the PC degenerate. The migratory pronuclei are then exchanged between two conjugants and fuse with the stationary pronuclei to form synkarya, which undergo three successive divisions (postzygotic divisions). However, little is known about the surviving mechanism of the PC nuclei. In the current study, stage-specific appearance of cytoplasmic microtubules (cMTs) was indicated during the third prezygotic division by immunofluorescence labeling with anti-alpha tubulin antibodies surrounding the surviving nuclei, including the PC nuclei and the two types of prospective pronuclei. This suggested that cMTs were involved in the formation of a physical barrier, whose function may relate to sequestering and protecting the surviving nuclei from the major cytoplasm, where degeneration of extra-meiotic products occurs, another important nuclear event during the third prezygotic division.

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Section: Discussionmentioning

confidence: 89%