Nucleic acid amplification test (NAAT) conducted in a microfluidic chip to differentiate between various ginseng species

Oberc, Christopher; Sojoudi, Parsa; Li, Paul C. H.

doi:10.1039/d2an01960d

Cited by 3 publications

(4 citation statements)

References 41 publications

(65 reference statements)

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“…The success was previously shown when the N2 site was used for ginseng species differentiation using NAT based on hybridization [ 5 ], and NAAT based on ligase-based amplification [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…There are many genetic methods available that use single nucleotide polymorphisms (SNPs), e.g., nucleic acid test (NAT) using probe-PCR product strand hybridization [ [4] , [5] , [6] , [7] , [8] ], qPCR [ [9] , [10] , [11] ], and lesion induced DNA amplification (LIDA) [ [12] , [13] , [14] ]. These techniques, which are effective when the DNA samples have known single nucleotide polymorphisms (SNPs), require considerable optimization.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, work by Zhou, H et al on the dammarenediol-II synthase (DS) gene [ 3 , 15 ] has shown that the gene contains several SNP sites which vary between P. ginseng and P. quinquefolius . Inspired by this work, three known SNP sites have been confirmed for use in ginseng authentication using hybridization [ 5 , 6 ] and LIDA [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Next-generation DNA sequencing of Panax samples revealed new genotypes: Burrows-Wheeler Aligner, Python-based abundance and clustering analysis

Oberc,

2024

Heliyon

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“…The success was previously shown when the N2 site was used for ginseng species differentiation using NAT based on hybridization [ 5 ], and NAAT based on ligase-based amplification [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Next-generation DNA sequencing of Panax samples revealed new genotypes: Burrows-Wheeler Aligner, Python-based abundance and clustering analysis

Oberc,

2024

Heliyon

Self Cite

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“…Both P. quinquefolium and P. ginseng are valuable traditional Chinese medicinal herbs with similar biological active components and morphological characteristics; yet, they hold distinct therapeutic values [3,15]. To differentiate between these two plants, the Chinese Pharmacopoeia has established methods and standards for identification, including visual examination, microscopic analysis, silica gel plate testing of extracts, and quantification of major ginsenosides [1].…”

Section: Discussionmentioning

confidence: 99%

Rapid Discrimination of Panax quinquefolium and Panax ginseng Using the Proofman-Duplex-LMTIA Technique

Zhang,

Li,

Zhang

et al. 2023

Molecules

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This study aims to establish a rapid identification method based on the Proofman-LMTIA technique for distinguishing between Panax quinquefolium and Panax ginseng. By targeting specific 18S rDNA sequences, suitable primers and Proofman probes labeled FAM or JOE were designed for LMTIA. Initially, single-species-primer Proofman-LMTIA assays were performed separately for each ginseng type to optimize reaction temperature, assess sensitivity and specificity, and determine the detection limit. Subsequently, both sets of primers and their corresponding probes were combined in the same reaction system to further optimize reaction conditions, evaluate sensitivity, and assess stability. Finally, the developed Proofman-duplex-LMTIA technique was employed to detect P. quinquefolium and P. ginseng slices available in the market. Single-plex Proofman-LMTIA assays revealed that the optimal reaction temperature for both P. quinquefolium and P. ginseng was 62 °C. The sensitivity was as low as 1 pg/μL, with a detection limit of 0.1%, and both showed excellent specificity. The optimal temperature for Proofman-duplex-LMTIA assays was 58 °C. This method could simultaneously identify P. quinquefolium and P. ginseng. Testing 6 samples of P. ginseng and 11 samples of P. quinquefolium from the market resulted in a 100% positive rate for all samples. This study successfully established a rapid, simple, sensitive, and specific Proofman-duplex-LMTIA identification method for P. quinquefolium and P. ginseng. It provides an effective means for quality control of P. quinquefolium, P. ginseng, and related products.

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Development of a Spot Test (ST) Based on the Nucleic Acid Amplification Test (NAAT) for Ginseng Species Authentication

Sojoudi,

Oberc,

Tiffere

et al. 2024

Analytical Letters

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Nucleic acid amplification test (NAAT) conducted in a microfluidic chip to differentiate between various ginseng species

Abstract: Panax ginseng and Panax quinquefolius have different medicinal properties and market values; however, they can be difficult to distinguish from one another based on physical appearances alone. Therefore, a molecular...

Cited by 3 publications

References 41 publications

Next-generation DNA sequencing of Panax samples revealed new genotypes: Burrows-Wheeler Aligner, Python-based abundance and clustering analysis

Next-generation DNA sequencing of Panax samples revealed new genotypes: Burrows-Wheeler Aligner, Python-based abundance and clustering analysis

Rapid Discrimination of Panax quinquefolium and Panax ginseng Using the Proofman-Duplex-LMTIA Technique

Development of a Spot Test (ST) Based on the Nucleic Acid Amplification Test (NAAT) for Ginseng Species Authentication

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