Nucleic Acid Analysis

Christopoulos, Theodore K.

doi:10.1021/a19900161

Cited by 78 publications

(50 citation statements)

References 317 publications

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“…2 is the best-developed and most widely used method of nucleic acid amplification (1)(2)(3). It has opened new avenues of pathogen detection, often supporting or replacing the traditional laborious methods in the field of virology (4 -6).…”

Section: The Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Hybridization Assay at a Disposable Electrochemical Biosensor for the Attomole Detection of Amplified Human Cytomegalovirus DNA

Azek¹,

Grossiord²,

Joannes

et al. 2000

Analytical Biochemistry

154

113

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Section: The Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Hybridization Assay at a Disposable Electrochemical Biosensor for the Attomole Detection of Amplified Human Cytomegalovirus DNA

Azek¹,

Grossiord²,

Joannes

et al. 2000

Analytical Biochemistry

154

113

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“…[1][2][3][4][5][6][7][8] Over 5000 flavonoids have been isolated from plants, most of which are divided into subclasses, including anthocyanidins, flavanones, flavonols, flavones, and isoflavones. Among them, flavones are less common than flavonols, including the well-known flavones 5,6,7-trihydroxyflavone and 7, 8-dihydroxyflavone.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Two Flavonoids With Calf Thymus Dna: A Multi - Spectroscopic, Electrochemical and Molecular Modelling Approach

Prabhakaran

Jeyasundari²,

Vasantha³

et al. 2018

ECB

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Interaction of naturally occurring bioactive flavonoids 5,6,7-trihydroxyflavone (Baicalein) and 7,8-dihydroxyflavone (DHF) binding with calf thymus deoxyribose nucleic acid (dsDNA) was studied by employing UV absorption, fluorescence, circular dichroism, cyclic voltammetric and molecular modeling techniques. All studies were confirmed that the structural changes of DNA binding to the flavonoid. From the CV results positive shift in peak potential and increased peak current of the flavonoid in the presence of DNA and then the fluorescence quenching of DNA-flavonoids system indicated the intercalative mode of binding between flavonoid and DNA. CD studies suggest the conformational changes in DNA upon interaction with the flavonoids. Molecular docking simulation methods are used as tools to delineate the binding mode and probable location of the flavonoids and their effects on the stability and conformation of Ct-(ds) DNA. Furthermore, Baicalein can bind with more potential with Ct-(ds) DNA than DHF. This is helpful to understand the molecular aspects of binding mode and provides direction for the use and the design of new effective therapeutic agents. These results could provide useful information for insight into the pharmacological mechanism of flavonoids.

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“…The most common strategy for DNA hybridization detection is by using optical [1], mass sensitive [2] or electrochemical [3] transducers. The development of this type of devices may have applicability on disease diagnostics [4], food quality testing [5], environmental testing [6], among other areas. Electrochemical methods for DNA sensing offer advantages such as: easy and fast detection, high sensitivity, high selectivity, portability, low power consumption and low cost [3,7].…”

Section: Introductionmentioning

confidence: 99%

Single‐Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor

2010

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A gold surface modified with a self-assembled monolayer of 11-amino-1-undecanethiol (AUT) was used for the covalent immobilization of oxidized single-walled carbon nanotubes (SWNTs). The as-described SWNTs-modified substrate was subsequently used to attach single-stranded deoxyribonucleic acid (ssDNA) used as a substrate for DNA hybridization. Electrochemical impedance spectroscopy measurements were performed to follow the DNA hybridization process by using the redox couple [Fe(CN) 6 ] 3À/4À as a marker ion. Specifically, changes in charge transfer resistance obtained from the Nyquist plots were used as the sensing parameter of DNA hybridization. The substrate sensitivity towards changes in target DNA concentration, its selectivity toward different DNA sequences and its reusability are successfully demonstrated in this report.

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Nucleic Acid Analysis

Cited by 78 publications

References 317 publications

Hybridization Assay at a Disposable Electrochemical Biosensor for the Attomole Detection of Amplified Human Cytomegalovirus DNA

Hybridization Assay at a Disposable Electrochemical Biosensor for the Attomole Detection of Amplified Human Cytomegalovirus DNA

Interaction of Two Flavonoids With Calf Thymus Dna: A Multi - Spectroscopic, Electrochemical and Molecular Modelling Approach

Single‐Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor

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